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Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens

Water bath stunning is a common practice in commercial slaughterhouses. Such treatment is economic and in line with animal welfare practice. However, the conditions applied for the stunning process may vary from a slaughterhouse to another slaughterhouse. Such a loose regulation on the stunning proc...

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Main Authors: Abu Samah, Norshahida, Amid, Azura, Yusof, Faridah
Format: Article
Language:English
Published: IIUM Press 2011
Subjects:
TA164 Bioengineering
Online Access:http://irep.iium.edu.my/17405/1/over.pdf
http://irep.iium.edu.my/17405/
http://www.iium.edu.my/ejournal/index.php/iiumej/article/view/258/220
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spelling my.iium.irep.174052012-01-31T00:32:36Z http://irep.iium.edu.my/17405/ Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens Abu Samah, Norshahida Amid, Azura Yusof, Faridah TA164 Bioengineering Water bath stunning is a common practice in commercial slaughterhouses. Such treatment is economic and in line with animal welfare practice. However, the conditions applied for the stunning process may vary from a slaughterhouse to another slaughterhouse. Such a loose regulation on the stunning procedure has opened up doors for food adulteration such as over dose stunning. In this study, a simple and reliable approach using proteomics have been developed to study the effect of different currents and voltages in stunning on the protein expression of the chickens. Protein profiles of the chickens were constructed in order to detect any differences in protein expression and modifications. The different voltage studied were 10 V, 40 V and 70 V while the values for current studied were 0.25 A, 0.5 A, and 0.75 A. After the proteomics analyses using 2D Platinum ImageMaster 6.0 and Matrix-assisted laser desorption ionization- time of flight (MALDI TOF) spectrometry identification, Voltage dependent anion channel 2 (VDAC2) was identified to be over expressed in the muscle sample of over stunned chicken. The over expression of VDAC2 was confirmed at the transcriptional level of RNA expression. Real Time PCR showed that all over stunned samples contained higher mRNA expression level for VDAC2 genes. The mRNA level of VDAC2 was upregulated by 59.87 fold change when normalized with housekeeping gene. In conclusion, VDAC2 could serve as potential biomarkers for identification of electrically stimulated chickens. The existence of these biomarkers will help to monitor the slaughtering and stunning process in the future. It will revolutionize the food authentication field and give a new breathe to the meat industry. IIUM Press 2011 Article REM application/pdf en http://irep.iium.edu.my/17405/1/over.pdf Abu Samah, Norshahida and Amid, Azura and Yusof, Faridah (2011) Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens. IIUM Engineering Journal, 12 (4). pp. 213-221. ISSN 1511-788X http://www.iium.edu.my/ejournal/index.php/iiumej/article/view/258/220
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TA164 Bioengineering
spellingShingle TA164 Bioengineering
Abu Samah, Norshahida
Amid, Azura
Yusof, Faridah
Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
description Water bath stunning is a common practice in commercial slaughterhouses. Such treatment is economic and in line with animal welfare practice. However, the conditions applied for the stunning process may vary from a slaughterhouse to another slaughterhouse. Such a loose regulation on the stunning procedure has opened up doors for food adulteration such as over dose stunning. In this study, a simple and reliable approach using proteomics have been developed to study the effect of different currents and voltages in stunning on the protein expression of the chickens. Protein profiles of the chickens were constructed in order to detect any differences in protein expression and modifications. The different voltage studied were 10 V, 40 V and 70 V while the values for current studied were 0.25 A, 0.5 A, and 0.75 A. After the proteomics analyses using 2D Platinum ImageMaster 6.0 and Matrix-assisted laser desorption ionization- time of flight (MALDI TOF) spectrometry identification, Voltage dependent anion channel 2 (VDAC2) was identified to be over expressed in the muscle sample of over stunned chicken. The over expression of VDAC2 was confirmed at the transcriptional level of RNA expression. Real Time PCR showed that all over stunned samples contained higher mRNA expression level for VDAC2 genes. The mRNA level of VDAC2 was upregulated by 59.87 fold change when normalized with housekeeping gene. In conclusion, VDAC2 could serve as potential biomarkers for identification of electrically stimulated chickens. The existence of these biomarkers will help to monitor the slaughtering and stunning process in the future. It will revolutionize the food authentication field and give a new breathe to the meat industry.
format Article
author Abu Samah, Norshahida
Amid, Azura
Yusof, Faridah
author_facet Abu Samah, Norshahida
Amid, Azura
Yusof, Faridah
author_sort Abu Samah, Norshahida
title Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
title_short Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
title_full Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
title_fullStr Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
title_full_unstemmed Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
title_sort over expression of voltage dependent anion channel 2 (vdac2) in muscles of electrically stunned chickens
publisher IIUM Press
publishDate 2011
url http://irep.iium.edu.my/17405/1/over.pdf
http://irep.iium.edu.my/17405/
http://www.iium.edu.my/ejournal/index.php/iiumej/article/view/258/220
_version_ 1643607277506134016
score 13.211869

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