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Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification

To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill...

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Main Authors: Rashid, Shah S,, Alam, Md Zahangir, Abdulkarim, Mohd Ismail, Mohd. Salleh, Hamzah
Format: Article
Language:English
Published: Academic Journals 2011
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/14380/1/AJB-Rashid_et_al.pdf
http://irep.iium.edu.my/14380/
http://dx.doi.org/10.5897/AJB11.2745
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spelling my.iium.irep.143802021-07-14T01:48:19Z http://irep.iium.edu.my/14380/ Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification Rashid, Shah S, Alam, Md Zahangir Abdulkarim, Mohd Ismail Mohd. Salleh, Hamzah TP248.13 Biotechnology To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill effluent (POME) by the fungal strain, Trichoderma reesei RUT C-30. This study was conducted by using two different types of agents (physical and chemical). Heating, boiling and steaming are among the physical agents and different concentrations of nitric acid, sulfuric acid and sodium hydroxide (NaOH) were the chemical agents used for the pretreatment of EFB to enhance the enzymatic saccharification of EFB. NaOH was proved to be the best among all the pretreatment agents and 3% NaOH was far higher and 2.35 fold increment was achieved on the yield of reducing sugar (175.03 mg/g of EFB) after 96 h of saccharification. A maximum of 41.82% yield of reducing sugar was achieved with 5% (w/v) of EFB and 7% (v/v) of enzyme after 120 h of saccharification when eight important parameters, namely saccharification duration, EFB size, EFB dose, enzyme dose, Tween 80, triton 100, agitation and incubation temperature, were examined in an OFAT (one factor at-a-time) design. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/14380/1/AJB-Rashid_et_al.pdf Rashid, Shah S, and Alam, Md Zahangir and Abdulkarim, Mohd Ismail and Mohd. Salleh, Hamzah (2011) Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification. African Journal of Biotechnology, 10 (81). pp. 18728-18738. ISSN 1684–5315 http://dx.doi.org/10.5897/AJB11.2745 doi:10.5897/AJB11.2745
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Rashid, Shah S,
Alam, Md Zahangir
Abdulkarim, Mohd Ismail
Mohd. Salleh, Hamzah
Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
description To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill effluent (POME) by the fungal strain, Trichoderma reesei RUT C-30. This study was conducted by using two different types of agents (physical and chemical). Heating, boiling and steaming are among the physical agents and different concentrations of nitric acid, sulfuric acid and sodium hydroxide (NaOH) were the chemical agents used for the pretreatment of EFB to enhance the enzymatic saccharification of EFB. NaOH was proved to be the best among all the pretreatment agents and 3% NaOH was far higher and 2.35 fold increment was achieved on the yield of reducing sugar (175.03 mg/g of EFB) after 96 h of saccharification. A maximum of 41.82% yield of reducing sugar was achieved with 5% (w/v) of EFB and 7% (v/v) of enzyme after 120 h of saccharification when eight important parameters, namely saccharification duration, EFB size, EFB dose, enzyme dose, Tween 80, triton 100, agitation and incubation temperature, were examined in an OFAT (one factor at-a-time) design.
format Article
author Rashid, Shah S,
Alam, Md Zahangir
Abdulkarim, Mohd Ismail
Mohd. Salleh, Hamzah
author_facet Rashid, Shah S,
Alam, Md Zahangir
Abdulkarim, Mohd Ismail
Mohd. Salleh, Hamzah
author_sort Rashid, Shah S,
title Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
title_short Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
title_full Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
title_fullStr Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
title_full_unstemmed Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
title_sort development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
publisher Academic Journals
publishDate 2011
url http://irep.iium.edu.my/14380/1/AJB-Rashid_et_al.pdf
http://irep.iium.edu.my/14380/
http://dx.doi.org/10.5897/AJB11.2745
_version_ 1706956464047783936
score 13.211869

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