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Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)

Previously, tomato leaves were proved to be one of the potential anticancer agents. High performanceliquid chromatography (HPLC) and Fourier transform infrared (FTIR) spectroscopic instrumentation were used to predict the presence of group of metabolites and to ascertain the possibility of certain a...

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Main Authors: Wan Chik, Wan Dalila, Amid, Azura, Jamal, Parveen
Format: Article
Language:English
Published: Academic Journals 2011
Subjects:
QR Microbiology
Online Access:http://irep.iium.edu.my/13993/1/Chik_et_al.pdf
http://irep.iium.edu.my/13993/
http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Chik%20et%20al.pdf
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spelling my.iium.irep.139932013-07-02T08:24:39Z http://irep.iium.edu.my/13993/ Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR) Wan Chik, Wan Dalila Amid, Azura Jamal, Parveen QR Microbiology Previously, tomato leaves were proved to be one of the potential anticancer agents. High performanceliquid chromatography (HPLC) and Fourier transform infrared (FTIR) spectroscopic instrumentation were used to predict the presence of group of metabolites and to ascertain the possibility of certain absorption bands, in which most of the peaks in partially purified tomato (Solanum lycopersicon)leaves methanol extract are attributed to the specific functional groups. The extraction was carried outin a shake flask with 82% methanol, 1:10 (w/v) sample to solvent ratio, agitated at 22°C with 110 rpm within 24 h. Later, the extract was partially purified by column chromatography. HPLC was used to quantify the number of unknown component presents in the fraction. Then, a FT-IR Bruker Tensor 27 System was used during FTIR data acquisition. The collection of FTIR spectra was carried out at 16 scans with resolution of 4 cm -1 using strong apodization in the frequency regions of 4,000 to 650 cm-1.The results support the premise that HPLC and FTIR spectroscopy are efficient and accurate methods for determining major and minor components presents in the extract. Academic Journals 2011-12-16 Article REM application/pdf en http://irep.iium.edu.my/13993/1/Chik_et_al.pdf Wan Chik, Wan Dalila and Amid, Azura and Jamal, Parveen (2011) Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR). African Journal of Biotechnology, 10 (81). pp. 18666-18673. ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Chik%20et%20al.pdf 10.5897/AJB11.2736
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Wan Chik, Wan Dalila
Amid, Azura
Jamal, Parveen
Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
description Previously, tomato leaves were proved to be one of the potential anticancer agents. High performanceliquid chromatography (HPLC) and Fourier transform infrared (FTIR) spectroscopic instrumentation were used to predict the presence of group of metabolites and to ascertain the possibility of certain absorption bands, in which most of the peaks in partially purified tomato (Solanum lycopersicon)leaves methanol extract are attributed to the specific functional groups. The extraction was carried outin a shake flask with 82% methanol, 1:10 (w/v) sample to solvent ratio, agitated at 22°C with 110 rpm within 24 h. Later, the extract was partially purified by column chromatography. HPLC was used to quantify the number of unknown component presents in the fraction. Then, a FT-IR Bruker Tensor 27 System was used during FTIR data acquisition. The collection of FTIR spectra was carried out at 16 scans with resolution of 4 cm -1 using strong apodization in the frequency regions of 4,000 to 650 cm-1.The results support the premise that HPLC and FTIR spectroscopy are efficient and accurate methods for determining major and minor components presents in the extract.
format Article
author Wan Chik, Wan Dalila
Amid, Azura
Jamal, Parveen
author_facet Wan Chik, Wan Dalila
Amid, Azura
Jamal, Parveen
author_sort Wan Chik, Wan Dalila
title Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
title_short Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
title_full Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
title_fullStr Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
title_full_unstemmed Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
title_sort predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (hplc) and fourier transform infrared (ftir)
publisher Academic Journals
publishDate 2011
url http://irep.iium.edu.my/13993/1/Chik_et_al.pdf
http://irep.iium.edu.my/13993/
http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Chik%20et%20al.pdf
_version_ 1643606837787885568
score 13.211869

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