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Effect of different media components and process conditions on the productivity of phenolic compounds from palm oil mill effluent by local isolated strain

The present investigation is an effort to develop an environmentally sound and cost effective fermentation process by introducing a new potential strain of Aspergillus niger which was isolated locally for the production of phenolics from palm oil mill effluent (POME). The selection of the potential...

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Main Authors: Idris, Zulkarnain Mohamed, Jamal, Parveen, Alam, Md. Zahangir
Format: Conference or Workshop Item
Language:English
Published: 2009
Subjects:
TA170 Environmental engineering
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/13893/1/MICOTRIBE_2009_137-145.pdf
http://irep.iium.edu.my/13893/
http://publicweb.unimap.edu.my/~MICOTriBE2009/
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spelling my.iium.irep.138932012-01-14T00:15:30Z http://irep.iium.edu.my/13893/ Effect of different media components and process conditions on the productivity of phenolic compounds from palm oil mill effluent by local isolated strain Idris, Zulkarnain Mohamed Jamal, Parveen Alam, Md. Zahangir TA170 Environmental engineering TP248.13 Biotechnology The present investigation is an effort to develop an environmentally sound and cost effective fermentation process by introducing a new potential strain of Aspergillus niger which was isolated locally for the production of phenolics from palm oil mill effluent (POME). The selection of the potential fungal strain was done previously by screening seven strains and strain A103, which gave the highest total phenolic content after 72 hours of fermentation at fixed media and process conditions was used in this study. To enhance the production of phenolic compounds, a study based on statistical design was employed. A two-level Plackett-Burman design was applied where 11 variables consisted of various media components and process conditions were studied for their influence on phenolics production. Out of 11 variables, sucrose, manganese sulfate (MnSO4), and temperature were identified as the most significant variables in improving phenolics production by A103 strain. Single factor optimization for the selected variables was performed to further the optimization for the phenolics production. After the optimization, total phenolic content increased significantly from 856.09 to 940.80 GAE mg/l. The optimum conditions were found at a temperature of 35.0°C, 6.0% (w/v) sucrose, and 2.5% (w/v) MnSO4, with other fixed parameters. Surprisingly, the scavenging activity of the fermented extract on 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical showed almost 95% of inhibition. The activity was higher compared to butylated hydroxytoluene (BHT), a synthetic antioxidant which showed only 91% of inhibition, with promising applications in food and pharmaceutical industries. 2009-12 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/13893/1/MICOTRIBE_2009_137-145.pdf Idris, Zulkarnain Mohamed and Jamal, Parveen and Alam, Md. Zahangir (2009) Effect of different media components and process conditions on the productivity of phenolic compounds from palm oil mill effluent by local isolated strain. In: Malaysian International Conference on Trends in Bioprocess Engineering (MICOTriBE 2009), 12-13 December 2009, PWTC, Kuala Lumpur. http://publicweb.unimap.edu.my/~MICOTriBE2009/
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TA170 Environmental engineering
TP248.13 Biotechnology
spellingShingle TA170 Environmental engineering
TP248.13 Biotechnology
Idris, Zulkarnain Mohamed
Jamal, Parveen
Alam, Md. Zahangir
Effect of different media components and process conditions on the productivity of phenolic compounds from palm oil mill effluent by local isolated strain
description The present investigation is an effort to develop an environmentally sound and cost effective fermentation process by introducing a new potential strain of Aspergillus niger which was isolated locally for the production of phenolics from palm oil mill effluent (POME). The selection of the potential fungal strain was done previously by screening seven strains and strain A103, which gave the highest total phenolic content after 72 hours of fermentation at fixed media and process conditions was used in this study. To enhance the production of phenolic compounds, a study based on statistical design was employed. A two-level Plackett-Burman design was applied where 11 variables consisted of various media components and process conditions were studied for their influence on phenolics production. Out of 11 variables, sucrose, manganese sulfate (MnSO4), and temperature were identified as the most significant variables in improving phenolics production by A103 strain. Single factor optimization for the selected variables was performed to further the optimization for the phenolics production. After the optimization, total phenolic content increased significantly from 856.09 to 940.80 GAE mg/l. The optimum conditions were found at a temperature of 35.0°C, 6.0% (w/v) sucrose, and 2.5% (w/v) MnSO4, with other fixed parameters. Surprisingly, the scavenging activity of the fermented extract on 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical showed almost 95% of inhibition. The activity was higher compared to butylated hydroxytoluene (BHT), a synthetic antioxidant which showed only 91% of inhibition, with promising applications in food and pharmaceutical industries.
format Conference or Workshop Item
author Idris, Zulkarnain Mohamed
Jamal, Parveen
Alam, Md. Zahangir
author_facet Idris, Zulkarnain Mohamed
Jamal, Parveen
Alam, Md. Zahangir
author_sort Idris, Zulkarnain Mohamed
title Effect of different media components and process conditions on the productivity of phenolic compounds from palm oil mill effluent by local isolated strain
title_short Effect of different media components and process conditions on the productivity of phenolic compounds from palm oil mill effluent by local isolated strain
title_full Effect of different media components and process conditions on the productivity of phenolic compounds from palm oil mill effluent by local isolated strain
title_fullStr Effect of different media components and process conditions on the productivity of phenolic compounds from palm oil mill effluent by local isolated strain
title_full_unstemmed Effect of different media components and process conditions on the productivity of phenolic compounds from palm oil mill effluent by local isolated strain
title_sort effect of different media components and process conditions on the productivity of phenolic compounds from palm oil mill effluent by local isolated strain
publishDate 2009
url http://irep.iium.edu.my/13893/1/MICOTRIBE_2009_137-145.pdf
http://irep.iium.edu.my/13893/
http://publicweb.unimap.edu.my/~MICOTriBE2009/
_version_ 1643606821993185280
score 13.211869

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