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Effect of Malaysian anti-diabetic plant extracts on adipocyte cells

Diabetic mellitus (DM)is a disease of worldwide significance and increasing prevalence. It is a metabolic disorder characterized by a predisposition to developing significantly raised blood glucose. The objective of this research was to evaluate the antidiabetic properties of medicinal plants ued in...

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Main Authors: Ali, Mohamed Zaffar, Bakhtiar, M. Taher, Darnis, Deny Susanti
Format: Conference or Workshop Item
Language:English
English
Published: 2010
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/12151/1/Icastcover.pdf
http://irep.iium.edu.my/12151/2/Icast3.pdf
http://irep.iium.edu.my/12151/
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spelling my.iium.irep.121512014-12-09T01:37:43Z http://irep.iium.edu.my/12151/ Effect of Malaysian anti-diabetic plant extracts on adipocyte cells Ali, Mohamed Zaffar Bakhtiar, M. Taher Darnis, Deny Susanti Q Science (General) Diabetic mellitus (DM)is a disease of worldwide significance and increasing prevalence. It is a metabolic disorder characterized by a predisposition to developing significantly raised blood glucose. The objective of this research was to evaluate the antidiabetic properties of medicinal plants ued in the treatment if type 2 diabetes. The selection of the plants was based on traditional reputation of usefulness in treating diabetes.Ethanolic extracts of Andrographis paniculata, Cinnamomum zeylanicum, Lagerstroemia speciosa, Momodica charantia, Orthosiphon stamineus, Phyllanthus amarus, Pithecellobium jiringa, Syzgium polyanthum, and Tinospora cirspa were selected. Insulin was used as a postive control. The plants were studied on the bioactive peptides (adipokines) using an in vitro model. Cells were seeded and counted everyday for ten days. The first step was to test the ability of the plants to induce predipocytes to adipocyte.cells. Prior to the testing, preadipocytes (3T3-L1) cells were differentiated to adipocytes by using a mixture of dexamethasone, isobutlymethylxanthine and the plant extract. Treated cells were stained by usingg oil red ) and the absorbance was read at 340 nm. 3T3-L1 preadipocytes exhibited the maximum growth at day-four(4.7x105 cells. Oil red O staining results showed that C.zeylanicum, L.speciosa leaves and S.polyanthum extracts have strong activity in inducing lipid formation and the others have moderate properties. The results of the study suggest the validiy of the plants for clinical usein treatmentof type 2 diabetes, after their toxicological investigation. This finding may lead to seriousresearch towardsa developing new and efficient drugs for diabetes. 2010-11-27 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/12151/1/Icastcover.pdf application/pdf en http://irep.iium.edu.my/12151/2/Icast3.pdf Ali, Mohamed Zaffar and Bakhtiar, M. Taher and Darnis, Deny Susanti (2010) Effect of Malaysian anti-diabetic plant extracts on adipocyte cells. In: International Conference on Advancement in Science and Technology (iCAST2010): The Science of Traditional Practices in Health and Disease, 26-29 Nov 2010, Kuantan, Malaysia. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Ali, Mohamed Zaffar
Bakhtiar, M. Taher
Darnis, Deny Susanti
Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
description Diabetic mellitus (DM)is a disease of worldwide significance and increasing prevalence. It is a metabolic disorder characterized by a predisposition to developing significantly raised blood glucose. The objective of this research was to evaluate the antidiabetic properties of medicinal plants ued in the treatment if type 2 diabetes. The selection of the plants was based on traditional reputation of usefulness in treating diabetes.Ethanolic extracts of Andrographis paniculata, Cinnamomum zeylanicum, Lagerstroemia speciosa, Momodica charantia, Orthosiphon stamineus, Phyllanthus amarus, Pithecellobium jiringa, Syzgium polyanthum, and Tinospora cirspa were selected. Insulin was used as a postive control. The plants were studied on the bioactive peptides (adipokines) using an in vitro model. Cells were seeded and counted everyday for ten days. The first step was to test the ability of the plants to induce predipocytes to adipocyte.cells. Prior to the testing, preadipocytes (3T3-L1) cells were differentiated to adipocytes by using a mixture of dexamethasone, isobutlymethylxanthine and the plant extract. Treated cells were stained by usingg oil red ) and the absorbance was read at 340 nm. 3T3-L1 preadipocytes exhibited the maximum growth at day-four(4.7x105 cells. Oil red O staining results showed that C.zeylanicum, L.speciosa leaves and S.polyanthum extracts have strong activity in inducing lipid formation and the others have moderate properties. The results of the study suggest the validiy of the plants for clinical usein treatmentof type 2 diabetes, after their toxicological investigation. This finding may lead to seriousresearch towardsa developing new and efficient drugs for diabetes.
format Conference or Workshop Item
author Ali, Mohamed Zaffar
Bakhtiar, M. Taher
Darnis, Deny Susanti
author_facet Ali, Mohamed Zaffar
Bakhtiar, M. Taher
Darnis, Deny Susanti
author_sort Ali, Mohamed Zaffar
title Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
title_short Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
title_full Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
title_fullStr Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
title_full_unstemmed Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
title_sort effect of malaysian anti-diabetic plant extracts on adipocyte cells
publishDate 2010
url http://irep.iium.edu.my/12151/1/Icastcover.pdf
http://irep.iium.edu.my/12151/2/Icast3.pdf
http://irep.iium.edu.my/12151/
_version_ 1643606585946144768
score 13.211869

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