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The investigation of bio-preservative properties in Plukenetia volubilis L. (Sacha Inchi) seeds protein extract for food spoilage Prevention

The importance of food quality and safety has long been recognized by the food industry to cater to food spoilage. Food spoilage is caused by the loss of food quality from its original organoleptic qualities due to chemical and biological spoilage processes that may impact customer acceptance. This...

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Main Authors: Md Zin, Noor Hasniza, Abdul Wahab, Widya, Ahmad, Mohammad Norazmi, Sapak, Zaiton, Azahar, Noor Suhana
Format: Article
Language:English
Published: The Malaysian Society of Applied Biology 2024
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/118036/7/118036_The%20investigation%20of%20bio-preservative%20properties.pdf
http://irep.iium.edu.my/118036/
https://jms.mabjournal.com/index.php/mab/article/view/3144
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spelling my.iium.irep.1180362025-01-03T11:33:20Z http://irep.iium.edu.my/118036/ The investigation of bio-preservative properties in Plukenetia volubilis L. (Sacha Inchi) seeds protein extract for food spoilage Prevention Md Zin, Noor Hasniza Abdul Wahab, Widya Ahmad, Mohammad Norazmi Sapak, Zaiton Azahar, Noor Suhana Q Science (General) The importance of food quality and safety has long been recognized by the food industry to cater to food spoilage. Food spoilage is caused by the loss of food quality from its original organoleptic qualities due to chemical and biological spoilage processes that may impact customer acceptance. This research aimed to evaluate the potential of Sacha inchi seeds protein as a multifunctional food preservative in controlling chemical (oxidation) and biological (microbial and protease activity) food spoilage. The antioxidant capacity revealed that the Sacha inchi seed protein has 73.72 ± 0.70% inhibition towards oxidation with the total phenolic content detected at the concentration of 463.13 ± 0.51 μg/mL in the protein extract. The tested food spoilage bacteria (Escherichia coli and Micrococcus luteus) and fungus (Colletotrichum gloeosporioides) were susceptible to Sacha inchi seed protein showing its potential antimicrobial properties. The Sacha inchi protein extract (46.65 μg/mL & 23.28 μg/mL) shows a significant inhibition for E. coli (30.5 mm) and M. luteus (33 mm), respectively, which indicated its effectiveness. Sacha inchi seed protein also displayed potential antifungal activities by suppressing the growth of C. gloeosporioides at inhibitory concentration percentages (IC%) of 1.5±0.12%, 5.85±3.89% and 5.90±1.98% after 2, 3 and 4-days of incubation, respectively. The caseinolytic plate assay revealed that Sacha inchi seed protein showed inhibition of trypsin digestion on casein with reduced inhibition diameter from 1.9±0.00 cm (negative control) to 1.05±0.00 cm. Following the effective protein separation by SDS-PAGE, zymography analysis revealed that a prominent protein band at 25 kDa showed protease inhibitory activity. This research contributes insights into the potential application of Sacha inchi seed extract as a bio-preservative in the food industry to combat food spoilage and it is aligned with SDG 2 for zero hunger The Malaysian Society of Applied Biology 2024-12-25 Article PeerReviewed application/pdf en http://irep.iium.edu.my/118036/7/118036_The%20investigation%20of%20bio-preservative%20properties.pdf Md Zin, Noor Hasniza and Abdul Wahab, Widya and Ahmad, Mohammad Norazmi and Sapak, Zaiton and Azahar, Noor Suhana (2024) The investigation of bio-preservative properties in Plukenetia volubilis L. (Sacha Inchi) seeds protein extract for food spoilage Prevention. Malaysian Applied Biology, 53 (6). pp. 87-96. ISSN 0126-8643 E-ISSN 2462-151X https://jms.mabjournal.com/index.php/mab/article/view/3144 10.55230/mabjournal.v53i6.9
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Md Zin, Noor Hasniza
Abdul Wahab, Widya
Ahmad, Mohammad Norazmi
Sapak, Zaiton
Azahar, Noor Suhana
The investigation of bio-preservative properties in Plukenetia volubilis L. (Sacha Inchi) seeds protein extract for food spoilage Prevention
description The importance of food quality and safety has long been recognized by the food industry to cater to food spoilage. Food spoilage is caused by the loss of food quality from its original organoleptic qualities due to chemical and biological spoilage processes that may impact customer acceptance. This research aimed to evaluate the potential of Sacha inchi seeds protein as a multifunctional food preservative in controlling chemical (oxidation) and biological (microbial and protease activity) food spoilage. The antioxidant capacity revealed that the Sacha inchi seed protein has 73.72 ± 0.70% inhibition towards oxidation with the total phenolic content detected at the concentration of 463.13 ± 0.51 μg/mL in the protein extract. The tested food spoilage bacteria (Escherichia coli and Micrococcus luteus) and fungus (Colletotrichum gloeosporioides) were susceptible to Sacha inchi seed protein showing its potential antimicrobial properties. The Sacha inchi protein extract (46.65 μg/mL & 23.28 μg/mL) shows a significant inhibition for E. coli (30.5 mm) and M. luteus (33 mm), respectively, which indicated its effectiveness. Sacha inchi seed protein also displayed potential antifungal activities by suppressing the growth of C. gloeosporioides at inhibitory concentration percentages (IC%) of 1.5±0.12%, 5.85±3.89% and 5.90±1.98% after 2, 3 and 4-days of incubation, respectively. The caseinolytic plate assay revealed that Sacha inchi seed protein showed inhibition of trypsin digestion on casein with reduced inhibition diameter from 1.9±0.00 cm (negative control) to 1.05±0.00 cm. Following the effective protein separation by SDS-PAGE, zymography analysis revealed that a prominent protein band at 25 kDa showed protease inhibitory activity. This research contributes insights into the potential application of Sacha inchi seed extract as a bio-preservative in the food industry to combat food spoilage and it is aligned with SDG 2 for zero hunger
format Article
author Md Zin, Noor Hasniza
Abdul Wahab, Widya
Ahmad, Mohammad Norazmi
Sapak, Zaiton
Azahar, Noor Suhana
author_facet Md Zin, Noor Hasniza
Abdul Wahab, Widya
Ahmad, Mohammad Norazmi
Sapak, Zaiton
Azahar, Noor Suhana
author_sort Md Zin, Noor Hasniza
title The investigation of bio-preservative properties in Plukenetia volubilis L. (Sacha Inchi) seeds protein extract for food spoilage Prevention
title_short The investigation of bio-preservative properties in Plukenetia volubilis L. (Sacha Inchi) seeds protein extract for food spoilage Prevention
title_full The investigation of bio-preservative properties in Plukenetia volubilis L. (Sacha Inchi) seeds protein extract for food spoilage Prevention
title_fullStr The investigation of bio-preservative properties in Plukenetia volubilis L. (Sacha Inchi) seeds protein extract for food spoilage Prevention
title_full_unstemmed The investigation of bio-preservative properties in Plukenetia volubilis L. (Sacha Inchi) seeds protein extract for food spoilage Prevention
title_sort investigation of bio-preservative properties in plukenetia volubilis l. (sacha inchi) seeds protein extract for food spoilage prevention
publisher The Malaysian Society of Applied Biology
publishDate 2024
url http://irep.iium.edu.my/118036/7/118036_The%20investigation%20of%20bio-preservative%20properties.pdf
http://irep.iium.edu.my/118036/
https://jms.mabjournal.com/index.php/mab/article/view/3144
_version_ 1821105175327145984
score 13.239859

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