In silico characterization and phylogenetic analysis of tryptophan decarboxylase(TDC) gene in Mitragyna speciosa
Mitragyna speciosa also known as “kratom’ or ‘ketum’, it is a medicinal herb. It has been recognized for its medicinal benefits, but it contains a toxic compound known as Mitragynine. This compound is known for its capability to cause adverse reactions such as addiction, misuse and death. Due to its...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kulliyyah of Science, International Islamic University Malaysia
2024
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/117862/7/117862_In%20silico%20characterization%20and%20phylogenetic%20analysis.pdf http://irep.iium.edu.my/117862/ https://journals.iium.edu.my/revival/index.php/revival/article/view/464 |
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| Summary: | Mitragyna speciosa also known as “kratom’ or ‘ketum’, it is a medicinal herb. It has been recognized for its medicinal benefits, but it contains a toxic compound known as Mitragynine. This compound is known for its capability to cause adverse reactions such as addiction, misuse and death. Due to its addictive properties, few countries have made the possession of M. speciosa illegal. The tryptophan decarboxylase (TDC) gene involved in the biosynthesis pathway to produce Mitragynine, which is known to contribute to the toxicity effects of M. speciosa. Thus, the aim of this study is to identify, characterize and analyze the TDC gene in M. speciosa using bioinformatic tools. In this study, the homologous search using Blastx revealed the highest percentage of identity, score, and E-value for Tryptophan decarboxylase M. speciosa protein with sequence ID AEQ01059.1. The presence of entry IPR002129 and PF00282 for the TDC M. speciosa protein sequence, which is available at positions 57-430 aa. Plus, 11 out of 100 homologous protein sequences from BLAST have been selected to construct the phylogenetic tree by using MEGA-X software, revealing that this protein belongs to the same family as Coffea arabica, Coffea euginioides, Ophiorrhiza pumila and Ophiorriza prostrata species, which is the Rubiaceae family. Moreover, physicochemical characteristics by Protparam revealed that TDC M. speciosa stable and exhibit hydrophilic nature. Thus, the findings of this work will contribute to better knowledge of TDC M. speciosa protein as well as related information regarding the biosynthesis pathway of Mitragynine alkaloid that contributes to the toxicity effect in M. speciosa. |
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