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Water extract have superior cytotoxic effect than ethanolic extract of clinacanthus nutans leaves in breast cancer stem cells

Background: The rapid development of medical technology in managing breast cancer patients still cannot solve the problem of recurrence and resistance. One of the causes of recurrence and molecular resistance is the presence of breast cancer stem cells (BCSCs). Clinacanthus nutans (C.nutans) is a...

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Main Authors: Syarifah, Siti, Rambe, Aldy S., Putra, Agung, Ichwan, M, Pane, Yunita Sari, Muhar, Adi Muradi, Khatib, Alfi, Munir, Delfitri, Rusda, M, Amin, Mustafa
Format: Article
Language:English
English
Published: Avicena Publishing 2024
Subjects:
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/117033/1/117033_Water%20extract%20have%20superior%20cytotoxic%20effect.pdf
http://irep.iium.edu.my/117033/2/117033_Water%20extract%20have%20superior%20cytotoxic%20effect_Scopus.pdf
http://irep.iium.edu.my/117033/
https://actainformmed.org/issue/2024-32-1/
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spelling my.iium.irep.1170332024-12-26T00:07:34Z http://irep.iium.edu.my/117033/ Water extract have superior cytotoxic effect than ethanolic extract of clinacanthus nutans leaves in breast cancer stem cells Syarifah, Siti Rambe, Aldy S. Putra, Agung Ichwan, M Pane, Yunita Sari Muhar, Adi Muradi Khatib, Alfi Munir, Delfitri Rusda, M Amin, Mustafa RS Pharmacy and materia medica Background: The rapid development of medical technology in managing breast cancer patients still cannot solve the problem of recurrence and resistance. One of the causes of recurrence and molecular resistance is the presence of breast cancer stem cells (BCSCs). Clinacanthus nutans (C.nutans) is a plant found in Medan, North Sumatra, Indonesia. This plant is believed to have anticancer activity in community. Objective: Our study aimed to assess phytochemical of C.nutans leaves, isolate breast cancer stem cells and determine the cytotoxic effects of the ethanolic extract and water extract of C.nutans leaves on breast cancer stem cells at 24, 48, and 72 h of observation. Methods: We underwent the cytotoxic test by using MTT assay and isolated breast cancer stem cells by using MACS and validated them by mammosphere test. Results: We found alkaloids, flavonoids, glycosides and tannins in simplicia and all extracts. BCSCs was valid with the diameter of the mammosphere BCSCs was > 60 µm. The IC50 values of 100%, 60%, 40%, 20% EE, and WE of C.nutans leaves were 227.30; 46.05; 31.12; 98.54, and 16.16 µg/ml respectively in the first 24 hours. In administering WE of C.nutans leaves, BCSCs viability was decreased at 24,48 and 72 hours of observation, namely 69.29±26%; 75.82 ± 21.02% and 38.94±9.34 % (p < 0.0001). Conclusion: The WE of C.nutans leaves had more substantial cytotoxic potential against BCSCs than the EE. The capability of WE C.nutans leaves to suppress BCSC’s viability was time-dependent. The anticancer activity were believed originate from alkaloid and flavonoid group. Avicena Publishing 2024-03-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/117033/1/117033_Water%20extract%20have%20superior%20cytotoxic%20effect.pdf application/pdf en http://irep.iium.edu.my/117033/2/117033_Water%20extract%20have%20superior%20cytotoxic%20effect_Scopus.pdf Syarifah, Siti and Rambe, Aldy S. and Putra, Agung and Ichwan, M and Pane, Yunita Sari and Muhar, Adi Muradi and Khatib, Alfi and Munir, Delfitri and Rusda, M and Amin, Mustafa (2024) Water extract have superior cytotoxic effect than ethanolic extract of clinacanthus nutans leaves in breast cancer stem cells. Acta Informatica Medica, 32 (1). pp. 4-10. ISSN 0353-8109 E-ISSN 1986-5988 https://actainformmed.org/issue/2024-32-1/ 10.5455/aim.2024.32.4-10
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Syarifah, Siti
Rambe, Aldy S.
Putra, Agung
Ichwan, M
Pane, Yunita Sari
Muhar, Adi Muradi
Khatib, Alfi
Munir, Delfitri
Rusda, M
Amin, Mustafa
Water extract have superior cytotoxic effect than ethanolic extract of clinacanthus nutans leaves in breast cancer stem cells
description Background: The rapid development of medical technology in managing breast cancer patients still cannot solve the problem of recurrence and resistance. One of the causes of recurrence and molecular resistance is the presence of breast cancer stem cells (BCSCs). Clinacanthus nutans (C.nutans) is a plant found in Medan, North Sumatra, Indonesia. This plant is believed to have anticancer activity in community. Objective: Our study aimed to assess phytochemical of C.nutans leaves, isolate breast cancer stem cells and determine the cytotoxic effects of the ethanolic extract and water extract of C.nutans leaves on breast cancer stem cells at 24, 48, and 72 h of observation. Methods: We underwent the cytotoxic test by using MTT assay and isolated breast cancer stem cells by using MACS and validated them by mammosphere test. Results: We found alkaloids, flavonoids, glycosides and tannins in simplicia and all extracts. BCSCs was valid with the diameter of the mammosphere BCSCs was > 60 µm. The IC50 values of 100%, 60%, 40%, 20% EE, and WE of C.nutans leaves were 227.30; 46.05; 31.12; 98.54, and 16.16 µg/ml respectively in the first 24 hours. In administering WE of C.nutans leaves, BCSCs viability was decreased at 24,48 and 72 hours of observation, namely 69.29±26%; 75.82 ± 21.02% and 38.94±9.34 % (p < 0.0001). Conclusion: The WE of C.nutans leaves had more substantial cytotoxic potential against BCSCs than the EE. The capability of WE C.nutans leaves to suppress BCSC’s viability was time-dependent. The anticancer activity were believed originate from alkaloid and flavonoid group.
format Article
author Syarifah, Siti
Rambe, Aldy S.
Putra, Agung
Ichwan, M
Pane, Yunita Sari
Muhar, Adi Muradi
Khatib, Alfi
Munir, Delfitri
Rusda, M
Amin, Mustafa
author_facet Syarifah, Siti
Rambe, Aldy S.
Putra, Agung
Ichwan, M
Pane, Yunita Sari
Muhar, Adi Muradi
Khatib, Alfi
Munir, Delfitri
Rusda, M
Amin, Mustafa
author_sort Syarifah, Siti
title Water extract have superior cytotoxic effect than ethanolic extract of clinacanthus nutans leaves in breast cancer stem cells
title_short Water extract have superior cytotoxic effect than ethanolic extract of clinacanthus nutans leaves in breast cancer stem cells
title_full Water extract have superior cytotoxic effect than ethanolic extract of clinacanthus nutans leaves in breast cancer stem cells
title_fullStr Water extract have superior cytotoxic effect than ethanolic extract of clinacanthus nutans leaves in breast cancer stem cells
title_full_unstemmed Water extract have superior cytotoxic effect than ethanolic extract of clinacanthus nutans leaves in breast cancer stem cells
title_sort water extract have superior cytotoxic effect than ethanolic extract of clinacanthus nutans leaves in breast cancer stem cells
publisher Avicena Publishing
publishDate 2024
url http://irep.iium.edu.my/117033/1/117033_Water%20extract%20have%20superior%20cytotoxic%20effect.pdf
http://irep.iium.edu.my/117033/2/117033_Water%20extract%20have%20superior%20cytotoxic%20effect_Scopus.pdf
http://irep.iium.edu.my/117033/
https://actainformmed.org/issue/2024-32-1/
_version_ 1819909415762919424
score 13.244413

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