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Inhibitory effect of (2S)-Pinocembrin from goniothalamus macrophyllus on the prostaglandin E2 production in macrophage cell lines

Pinocembrin (PCB), a flavonoid known for its anti-inflammatory properties, has been approved for various clinical trial applications. To evaluate deeper into the anti-inflammatory potential of the specific enantiomer of natural PCB, we conducted the first investigation into the efficacy of the pure...

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Main Authors: Yuda Teruna, Hilwan, Rullah, Kamal, Hendra, Rudi, Utami, Rahayu, Islami, Deri, Mohd Faudzi, Siti Munirah, Mohd Aluwi, Mohd Fadhlizil Fasihi, Lam, Kok Wai
Format: Article
Language:English
English
Published: 2024
Subjects:
RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:http://irep.iium.edu.my/116987/1/116987_Inhibitory%20effect%20of%20%282S%29-Pinocembrin%20from%20goniothalamus%20macrophyllus.pdf
http://irep.iium.edu.my/116987/2/116987_Inhibitory%20effect%20of%20%282S%29-Pinocembrin%20from%20goniothalamus%20macrophyllus_WoS.pdf
http://irep.iium.edu.my/116987/
https://onlinelibrary.wiley.com/doi/10.1155/2024/8811022
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spelling my.iium.irep.1169872024-12-20T08:04:36Z http://irep.iium.edu.my/116987/ Inhibitory effect of (2S)-Pinocembrin from goniothalamus macrophyllus on the prostaglandin E2 production in macrophage cell lines Yuda Teruna, Hilwan Rullah, Kamal Hendra, Rudi Utami, Rahayu Islami, Deri Mohd Faudzi, Siti Munirah Mohd Aluwi, Mohd Fadhlizil Fasihi Lam, Kok Wai RS403 Materia Medica-Pharmaceutical Chemistry Pinocembrin (PCB), a flavonoid known for its anti-inflammatory properties, has been approved for various clinical trial applications. To evaluate deeper into the anti-inflammatory potential of the specific enantiomer of natural PCB, we conducted the first investigation into the efficacy of the pure enantiomer (2S)-PCB in modulating inflammatory mediators induced by lipopolysaccharide (LPS) in both murine RAW 264.7 and human U937 macrophage cell lines. This particular compound was isolated from Goniothalamus macrophyllus (Annonaceae), a native plant of Indonesia. This plant has been used traditionally as an herbal medicine to alleviate inflammation. (2S)-PCB was isolated from the stem bark of G. macrophyllus by defatting with n-hexane followed by maceration with methanol. Purification was performed using several chromatographic techniques. The absolute configuration was determined using electronic circular dichroism (ECD) spectroscopy. This compound was then tested for its inhibitory activity on prostaglandin E2 (PGE2) and subjected to docking simulations. The results indicated that (2S)-PCB significantly suppressed the production of PGE2 induced by LPS in both RAW 264.7 and U937 cell lines. The docking simulations revealed that (2S)-PCB reduced PGE2 levels by suppressing mitogen-activated protein kinase (MAPK) activation through inhibiting p38 and extracellular signal-regulated kinases (ERK). These findings suggest that the compound may prevent worsening of septic shock caused by bacterial infection. 2024-10-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/116987/1/116987_Inhibitory%20effect%20of%20%282S%29-Pinocembrin%20from%20goniothalamus%20macrophyllus.pdf application/pdf en http://irep.iium.edu.my/116987/2/116987_Inhibitory%20effect%20of%20%282S%29-Pinocembrin%20from%20goniothalamus%20macrophyllus_WoS.pdf Yuda Teruna, Hilwan and Rullah, Kamal and Hendra, Rudi and Utami, Rahayu and Islami, Deri and Mohd Faudzi, Siti Munirah and Mohd Aluwi, Mohd Fadhlizil Fasihi and Lam, Kok Wai (2024) Inhibitory effect of (2S)-Pinocembrin from goniothalamus macrophyllus on the prostaglandin E2 production in macrophage cell lines. Advances in Pharmacological and Pharmaceutical Sciences. ISSN 2633-4690 https://onlinelibrary.wiley.com/doi/10.1155/2024/8811022 10.1155/2024/8811022
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle RS403 Materia Medica-Pharmaceutical Chemistry
Yuda Teruna, Hilwan
Rullah, Kamal
Hendra, Rudi
Utami, Rahayu
Islami, Deri
Mohd Faudzi, Siti Munirah
Mohd Aluwi, Mohd Fadhlizil Fasihi
Lam, Kok Wai
Inhibitory effect of (2S)-Pinocembrin from goniothalamus macrophyllus on the prostaglandin E2 production in macrophage cell lines
description Pinocembrin (PCB), a flavonoid known for its anti-inflammatory properties, has been approved for various clinical trial applications. To evaluate deeper into the anti-inflammatory potential of the specific enantiomer of natural PCB, we conducted the first investigation into the efficacy of the pure enantiomer (2S)-PCB in modulating inflammatory mediators induced by lipopolysaccharide (LPS) in both murine RAW 264.7 and human U937 macrophage cell lines. This particular compound was isolated from Goniothalamus macrophyllus (Annonaceae), a native plant of Indonesia. This plant has been used traditionally as an herbal medicine to alleviate inflammation. (2S)-PCB was isolated from the stem bark of G. macrophyllus by defatting with n-hexane followed by maceration with methanol. Purification was performed using several chromatographic techniques. The absolute configuration was determined using electronic circular dichroism (ECD) spectroscopy. This compound was then tested for its inhibitory activity on prostaglandin E2 (PGE2) and subjected to docking simulations. The results indicated that (2S)-PCB significantly suppressed the production of PGE2 induced by LPS in both RAW 264.7 and U937 cell lines. The docking simulations revealed that (2S)-PCB reduced PGE2 levels by suppressing mitogen-activated protein kinase (MAPK) activation through inhibiting p38 and extracellular signal-regulated kinases (ERK). These findings suggest that the compound may prevent worsening of septic shock caused by bacterial infection.
format Article
author Yuda Teruna, Hilwan
Rullah, Kamal
Hendra, Rudi
Utami, Rahayu
Islami, Deri
Mohd Faudzi, Siti Munirah
Mohd Aluwi, Mohd Fadhlizil Fasihi
Lam, Kok Wai
author_facet Yuda Teruna, Hilwan
Rullah, Kamal
Hendra, Rudi
Utami, Rahayu
Islami, Deri
Mohd Faudzi, Siti Munirah
Mohd Aluwi, Mohd Fadhlizil Fasihi
Lam, Kok Wai
author_sort Yuda Teruna, Hilwan
title Inhibitory effect of (2S)-Pinocembrin from goniothalamus macrophyllus on the prostaglandin E2 production in macrophage cell lines
title_short Inhibitory effect of (2S)-Pinocembrin from goniothalamus macrophyllus on the prostaglandin E2 production in macrophage cell lines
title_full Inhibitory effect of (2S)-Pinocembrin from goniothalamus macrophyllus on the prostaglandin E2 production in macrophage cell lines
title_fullStr Inhibitory effect of (2S)-Pinocembrin from goniothalamus macrophyllus on the prostaglandin E2 production in macrophage cell lines
title_full_unstemmed Inhibitory effect of (2S)-Pinocembrin from goniothalamus macrophyllus on the prostaglandin E2 production in macrophage cell lines
title_sort inhibitory effect of (2s)-pinocembrin from goniothalamus macrophyllus on the prostaglandin e2 production in macrophage cell lines
publishDate 2024
url http://irep.iium.edu.my/116987/1/116987_Inhibitory%20effect%20of%20%282S%29-Pinocembrin%20from%20goniothalamus%20macrophyllus.pdf
http://irep.iium.edu.my/116987/2/116987_Inhibitory%20effect%20of%20%282S%29-Pinocembrin%20from%20goniothalamus%20macrophyllus_WoS.pdf
http://irep.iium.edu.my/116987/
https://onlinelibrary.wiley.com/doi/10.1155/2024/8811022
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score 13.223943

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