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Analysis of Artabotrys hexapetalus stem bark and leaf ethanol extracts as α‑glucosidase inhibitors: in vitro analysis, LC‑MS/MS, machine learning, and molecular docking

Artabotrys hexapetalus (L.f.) Bhandari, Annonaceae, is one of Artabotrys species found in Asia, such as Sri Lanka, India, China, Malaysia, Indonesia, and other Southeast Asian countries. This plant is traditionally used as an herbal medicine. This research explored the potential of A. hexapetalus l...

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Main Authors: Rosa, Dela, Elya, Berna, Hanafi, Muhammad, Khatib, Alfi, Halim, Yuniwaty, Surya, Muhammad Imam
Format: Article
Language:English
English
English
Published: Springer Science and Business Media Deutschland GmbH 2023
Subjects:
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/116980/7/116980_Analysis%20of%20Artabotrys%20hexapetalus%20stem%20bark.pdf
http://irep.iium.edu.my/116980/8/116980_Analysis%20of%20Artabotrys%20hexapetalus%20stem%20bark_Scopus.pdf
http://irep.iium.edu.my/116980/9/116980_Analysis%20of%20Artabotrys%20hexapetalus%20stem%20bark_WoS.pdf
http://irep.iium.edu.my/116980/
https://link.springer.com/article/10.1007/s43450-023-00494-4
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spelling my.iium.irep.1169802024-12-20T07:36:47Z http://irep.iium.edu.my/116980/ Analysis of Artabotrys hexapetalus stem bark and leaf ethanol extracts as α‑glucosidase inhibitors: in vitro analysis, LC‑MS/MS, machine learning, and molecular docking Rosa, Dela Elya, Berna Hanafi, Muhammad Khatib, Alfi Halim, Yuniwaty Surya, Muhammad Imam RS Pharmacy and materia medica Artabotrys hexapetalus (L.f.) Bhandari, Annonaceae, is one of Artabotrys species found in Asia, such as Sri Lanka, India, China, Malaysia, Indonesia, and other Southeast Asian countries. This plant is traditionally used as an herbal medicine. This research explored the potential of A. hexapetalus leaf and stem bark ethanol extract to treat type 2 diabetes by inhibiting theactivity of α-glucosidase, including its relationship with the antioxidant activity, phenolic content, and flavonoid content. The analysis used α-glucosidase inhibition assay, 2,2-diphenyl-1-picrylhydrazyl free radical inhibition assay, ferric reducing antioxidant power assay, total phenolic content, total flavonoid content, liquid chromatography-tandem mass chromatography, and molecular docking analysis. Results showed that stem bark extract had a moderate α-glucosidase inhibitory activity with an IC50 value of 47.084 ppm. In contrast, the leaf extract had a weak α-glucosidase inhibitory activity with an IC50value of 104.755 ppm. Random permutation in random forest simulation was used to predict the factors contributing to the α-glucosidase inhibition. For stem bark and leaf extracts, the α-glucosidase inhibition activity was predicted to be influenced by antioxidant activity and phenolic compounds. Comparisons between various analyses were shown to corroborate the random permutation results. Nevertheless, since the major identified active compound that likely played a role in α-glucosidase inhibition in stem bark was from terpene groups, it was predicted that some major compounds with high α-glucosidase inhibitory and antioxidant activities in stem bark were yet to be identified. Springer Science and Business Media Deutschland GmbH 2023-12-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/116980/7/116980_Analysis%20of%20Artabotrys%20hexapetalus%20stem%20bark.pdf application/pdf en http://irep.iium.edu.my/116980/8/116980_Analysis%20of%20Artabotrys%20hexapetalus%20stem%20bark_Scopus.pdf application/pdf en http://irep.iium.edu.my/116980/9/116980_Analysis%20of%20Artabotrys%20hexapetalus%20stem%20bark_WoS.pdf Rosa, Dela and Elya, Berna and Hanafi, Muhammad and Khatib, Alfi and Halim, Yuniwaty and Surya, Muhammad Imam (2023) Analysis of Artabotrys hexapetalus stem bark and leaf ethanol extracts as α‑glucosidase inhibitors: in vitro analysis, LC‑MS/MS, machine learning, and molecular docking. Revista Brasileira de Farmacognosia, 34 (2). pp. 386-396. ISSN 1981528X https://link.springer.com/article/10.1007/s43450-023-00494-4 10.1007/s43450-023-00494-4
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Rosa, Dela
Elya, Berna
Hanafi, Muhammad
Khatib, Alfi
Halim, Yuniwaty
Surya, Muhammad Imam
Analysis of Artabotrys hexapetalus stem bark and leaf ethanol extracts as α‑glucosidase inhibitors: in vitro analysis, LC‑MS/MS, machine learning, and molecular docking
description Artabotrys hexapetalus (L.f.) Bhandari, Annonaceae, is one of Artabotrys species found in Asia, such as Sri Lanka, India, China, Malaysia, Indonesia, and other Southeast Asian countries. This plant is traditionally used as an herbal medicine. This research explored the potential of A. hexapetalus leaf and stem bark ethanol extract to treat type 2 diabetes by inhibiting theactivity of α-glucosidase, including its relationship with the antioxidant activity, phenolic content, and flavonoid content. The analysis used α-glucosidase inhibition assay, 2,2-diphenyl-1-picrylhydrazyl free radical inhibition assay, ferric reducing antioxidant power assay, total phenolic content, total flavonoid content, liquid chromatography-tandem mass chromatography, and molecular docking analysis. Results showed that stem bark extract had a moderate α-glucosidase inhibitory activity with an IC50 value of 47.084 ppm. In contrast, the leaf extract had a weak α-glucosidase inhibitory activity with an IC50value of 104.755 ppm. Random permutation in random forest simulation was used to predict the factors contributing to the α-glucosidase inhibition. For stem bark and leaf extracts, the α-glucosidase inhibition activity was predicted to be influenced by antioxidant activity and phenolic compounds. Comparisons between various analyses were shown to corroborate the random permutation results. Nevertheless, since the major identified active compound that likely played a role in α-glucosidase inhibition in stem bark was from terpene groups, it was predicted that some major compounds with high α-glucosidase inhibitory and antioxidant activities in stem bark were yet to be identified.
format Article
author Rosa, Dela
Elya, Berna
Hanafi, Muhammad
Khatib, Alfi
Halim, Yuniwaty
Surya, Muhammad Imam
author_facet Rosa, Dela
Elya, Berna
Hanafi, Muhammad
Khatib, Alfi
Halim, Yuniwaty
Surya, Muhammad Imam
author_sort Rosa, Dela
title Analysis of Artabotrys hexapetalus stem bark and leaf ethanol extracts as α‑glucosidase inhibitors: in vitro analysis, LC‑MS/MS, machine learning, and molecular docking
title_short Analysis of Artabotrys hexapetalus stem bark and leaf ethanol extracts as α‑glucosidase inhibitors: in vitro analysis, LC‑MS/MS, machine learning, and molecular docking
title_full Analysis of Artabotrys hexapetalus stem bark and leaf ethanol extracts as α‑glucosidase inhibitors: in vitro analysis, LC‑MS/MS, machine learning, and molecular docking
title_fullStr Analysis of Artabotrys hexapetalus stem bark and leaf ethanol extracts as α‑glucosidase inhibitors: in vitro analysis, LC‑MS/MS, machine learning, and molecular docking
title_full_unstemmed Analysis of Artabotrys hexapetalus stem bark and leaf ethanol extracts as α‑glucosidase inhibitors: in vitro analysis, LC‑MS/MS, machine learning, and molecular docking
title_sort analysis of artabotrys hexapetalus stem bark and leaf ethanol extracts as α‑glucosidase inhibitors: in vitro analysis, lc‑ms/ms, machine learning, and molecular docking
publisher Springer Science and Business Media Deutschland GmbH
publishDate 2023
url http://irep.iium.edu.my/116980/7/116980_Analysis%20of%20Artabotrys%20hexapetalus%20stem%20bark.pdf
http://irep.iium.edu.my/116980/8/116980_Analysis%20of%20Artabotrys%20hexapetalus%20stem%20bark_Scopus.pdf
http://irep.iium.edu.my/116980/9/116980_Analysis%20of%20Artabotrys%20hexapetalus%20stem%20bark_WoS.pdf
http://irep.iium.edu.my/116980/
https://link.springer.com/article/10.1007/s43450-023-00494-4
_version_ 1819909412939104256
score 13.223943

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