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Urine metabolomics in dengue infection: elucidating hexadecane as a potential biomarker

Introduction: Dengue infection remains a major global health concern, necessitating rapid, non-invasive, and universally accessible diagnostic methods. Existing diagnostic modalities fail to simultaneously address the critical aspects of sensitivity, specificity, efficiency, and cost-effectiveness....

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Main Authors: Abd Rahman, Izzat Zulhilmi, Wan Omar, Wan Fatein Nabeila, Abdullah, Aszrin
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2024
Subjects:
QP Physiology
RA643 Communicable Diseases and Public Health
Online Access:http://irep.iium.edu.my/115259/1/115259_Urine%20metabolomics%20in%20dengue%20infection.pdf
http://irep.iium.edu.my/115259/
https://medicineandhealthukm.com/sites/medicineandhealthukm.com/files/article/2024/37th_mspp_annual_scietific_meeting_pdf_15312.pdf
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spelling my.iium.irep.1152592024-11-15T07:43:24Z http://irep.iium.edu.my/115259/ Urine metabolomics in dengue infection: elucidating hexadecane as a potential biomarker Abd Rahman, Izzat Zulhilmi Wan Omar, Wan Fatein Nabeila Abdullah, Aszrin QP Physiology RA643 Communicable Diseases and Public Health Introduction: Dengue infection remains a major global health concern, necessitating rapid, non-invasive, and universally accessible diagnostic methods. Existing diagnostic modalities fail to simultaneously address the critical aspects of sensitivity, specificity, efficiency, and cost-effectiveness. The NS1 antigen detection test exhibits low sensitivity, while serology tests carry a high risk of false negativity due to low IgM titres in the early phase of infection. Urine-based metabolic profiling has emerged as a promising approach for the identification of specific disease biomarkers. This study aims to identify urinary metabolites associated with dengue infection, leveraging these biomarkers to develop a urine-based dengue test kit. Alkane group metabolites may rise as they act as energy substrate to assist viral replication. Materials and method: We analysed urine samples from 30 dengue-infected patients and 30 control subjects using Gas Chromatography-Mass Spectrometry (GC-MS). The analysis focused on identifying distinct metabolites based on retention time (RT) and similarity index (SI), with the quantification of their concentrations using peak area. Results: Our findings revealed a significant presence of hexadecane in the urine samples of dengue-infected subjects (p<0.01) compared to control subjects. This metabolite was characterized by a RT of 20.95 + 2.23 minutes, a SI of 85.50 + 5.00%, and a peak area of 1360566.25 + 1066618.37 a.u. Conclusion: The marked detection of hexadecane specifically in dengue patients, highlights its potential as a key biomarker for dengue detection. This discovery establishes the foundation for the development of rapid, non-invasive urine-based dengue diagnostic tests. Universiti Kebangsaan Malaysia 2024-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/115259/1/115259_Urine%20metabolomics%20in%20dengue%20infection.pdf Abd Rahman, Izzat Zulhilmi and Wan Omar, Wan Fatein Nabeila and Abdullah, Aszrin (2024) Urine metabolomics in dengue infection: elucidating hexadecane as a potential biomarker. Medicine & Health, 19 (5 (Suppl.)). p. 52. ISSN 1823-2140 E-ISSN 2289-5728 https://medicineandhealthukm.com/sites/medicineandhealthukm.com/files/article/2024/37th_mspp_annual_scietific_meeting_pdf_15312.pdf 10.17576/MH.2024.s1905
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QP Physiology
RA643 Communicable Diseases and Public Health
spellingShingle QP Physiology
RA643 Communicable Diseases and Public Health
Abd Rahman, Izzat Zulhilmi
Wan Omar, Wan Fatein Nabeila
Abdullah, Aszrin
Urine metabolomics in dengue infection: elucidating hexadecane as a potential biomarker
description Introduction: Dengue infection remains a major global health concern, necessitating rapid, non-invasive, and universally accessible diagnostic methods. Existing diagnostic modalities fail to simultaneously address the critical aspects of sensitivity, specificity, efficiency, and cost-effectiveness. The NS1 antigen detection test exhibits low sensitivity, while serology tests carry a high risk of false negativity due to low IgM titres in the early phase of infection. Urine-based metabolic profiling has emerged as a promising approach for the identification of specific disease biomarkers. This study aims to identify urinary metabolites associated with dengue infection, leveraging these biomarkers to develop a urine-based dengue test kit. Alkane group metabolites may rise as they act as energy substrate to assist viral replication. Materials and method: We analysed urine samples from 30 dengue-infected patients and 30 control subjects using Gas Chromatography-Mass Spectrometry (GC-MS). The analysis focused on identifying distinct metabolites based on retention time (RT) and similarity index (SI), with the quantification of their concentrations using peak area. Results: Our findings revealed a significant presence of hexadecane in the urine samples of dengue-infected subjects (p<0.01) compared to control subjects. This metabolite was characterized by a RT of 20.95 + 2.23 minutes, a SI of 85.50 + 5.00%, and a peak area of 1360566.25 + 1066618.37 a.u. Conclusion: The marked detection of hexadecane specifically in dengue patients, highlights its potential as a key biomarker for dengue detection. This discovery establishes the foundation for the development of rapid, non-invasive urine-based dengue diagnostic tests.
format Article
author Abd Rahman, Izzat Zulhilmi
Wan Omar, Wan Fatein Nabeila
Abdullah, Aszrin
author_facet Abd Rahman, Izzat Zulhilmi
Wan Omar, Wan Fatein Nabeila
Abdullah, Aszrin
author_sort Abd Rahman, Izzat Zulhilmi
title Urine metabolomics in dengue infection: elucidating hexadecane as a potential biomarker
title_short Urine metabolomics in dengue infection: elucidating hexadecane as a potential biomarker
title_full Urine metabolomics in dengue infection: elucidating hexadecane as a potential biomarker
title_fullStr Urine metabolomics in dengue infection: elucidating hexadecane as a potential biomarker
title_full_unstemmed Urine metabolomics in dengue infection: elucidating hexadecane as a potential biomarker
title_sort urine metabolomics in dengue infection: elucidating hexadecane as a potential biomarker
publisher Universiti Kebangsaan Malaysia
publishDate 2024
url http://irep.iium.edu.my/115259/1/115259_Urine%20metabolomics%20in%20dengue%20infection.pdf
http://irep.iium.edu.my/115259/
https://medicineandhealthukm.com/sites/medicineandhealthukm.com/files/article/2024/37th_mspp_annual_scietific_meeting_pdf_15312.pdf
_version_ 1816129616047243264
score 13.244413

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