Staff View: Biomarkers identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit

Biomarkers identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit

ABSTRACT: Dengue infection detection methods, namely dengue serology test and Real-Time PCR, are only available in clinical laboratories or healthcare facilities. This is time-consuming and inconvenient for patients. Thus, a non-invasive on-site urinary diagnostic kit for den...

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Main Authors:	Amid, Azura, Abdullah Sani, Muhamad Shirwan, Abdullah, Aszrin, Mohamed Bakrim, Norbaiyah, Wan Omar, Wan Fatein Nabeila, Zakaria, Nurul Aulia, Abdullah, Nor Zamzila, Nik Ahmad, Nik Nur Fatnoon
Format:	Article
Language:	English English
Published:	IIUM Press 2024
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/113961/2/113961_Biomarkers%20identification%20from%20urine.pdf http://irep.iium.edu.my/113961/3/113961_Biomarkers%20identification%20from%20urine_Scopus.pdf http://irep.iium.edu.my/113961/ https://journals.iium.edu.my/ejournal/index.php/iiumej https://doi.org/10.31436/iiumej.v25i2.2785
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spelling	my.iium.irep.1139612024-08-16T08:51:17Z http://irep.iium.edu.my/113961/ Biomarkers identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit Amid, Azura Abdullah Sani, Muhamad Shirwan Abdullah, Aszrin Mohamed Bakrim, Norbaiyah Wan Omar, Wan Fatein Nabeila Zakaria, Nurul Aulia Abdullah, Nor Zamzila Nik Ahmad, Nik Nur Fatnoon R Medicine (General) ABSTRACT: Dengue infection detection methods, namely dengue serology test and Real-Time PCR, are only available in clinical laboratories or healthcare facilities. This is time-consuming and inconvenient for patients. Thus, a non-invasive on-site urinary diagnostic kit for dengue infection that requires no trained personnel for blood extraction would be advantageous and warranted, albeit in developed or underdeveloped nations with limited resources. To develop the on-site diagnostic kit, an identification of biomarkers related to dengue infection is needed. This preliminary study aims to identify potential metabolite biomarkers from the urine of dengue patients using GCMS analysis. Urine samples of patients with serologically confirmed dengue infection were analyzed and compared with healthy volunteers. The study shows that the GCMS approach can identify differences in the urine of dengue patients from healthy volunteers. Heptacosane, Hexadecane, 2,4-bis(1,1-dimethylethyl) phenol, 2-bromooctane, tetradecane, hexyl octyl ester sulfurous acid, 2-benzoyl methyl ester benzoic acid, 2,9-dimethyl decane, and pentadecane were identified from the urine of dengue patients. The identified secretion of alkane may be a suitable candidate for colorimetric assay for the development of a user-friendly, home-screening rapid test kit for the detection of dengue infection. IIUM Press 2024-07-14 Article PeerReviewed application/pdf en http://irep.iium.edu.my/113961/2/113961_Biomarkers%20identification%20from%20urine.pdf application/pdf en http://irep.iium.edu.my/113961/3/113961_Biomarkers%20identification%20from%20urine_Scopus.pdf Amid, Azura and Abdullah Sani, Muhamad Shirwan and Abdullah, Aszrin and Mohamed Bakrim, Norbaiyah and Wan Omar, Wan Fatein Nabeila and Zakaria, Nurul Aulia and Abdullah, Nor Zamzila and Nik Ahmad, Nik Nur Fatnoon (2024) Biomarkers identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit. IIUM Engineering Journal, 25 (2). pp. 1-16. ISSN 1511-788X E-ISSN 2289-7860 https://journals.iium.edu.my/ejournal/index.php/iiumej https://doi.org/10.31436/iiumej.v25i2.2785
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Amid, Azura Abdullah Sani, Muhamad Shirwan Abdullah, Aszrin Mohamed Bakrim, Norbaiyah Wan Omar, Wan Fatein Nabeila Zakaria, Nurul Aulia Abdullah, Nor Zamzila Nik Ahmad, Nik Nur Fatnoon Biomarkers identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
description	ABSTRACT: Dengue infection detection methods, namely dengue serology test and Real-Time PCR, are only available in clinical laboratories or healthcare facilities. This is time-consuming and inconvenient for patients. Thus, a non-invasive on-site urinary diagnostic kit for dengue infection that requires no trained personnel for blood extraction would be advantageous and warranted, albeit in developed or underdeveloped nations with limited resources. To develop the on-site diagnostic kit, an identification of biomarkers related to dengue infection is needed. This preliminary study aims to identify potential metabolite biomarkers from the urine of dengue patients using GCMS analysis. Urine samples of patients with serologically confirmed dengue infection were analyzed and compared with healthy volunteers. The study shows that the GCMS approach can identify differences in the urine of dengue patients from healthy volunteers. Heptacosane, Hexadecane, 2,4-bis(1,1-dimethylethyl) phenol, 2-bromooctane, tetradecane, hexyl octyl ester sulfurous acid, 2-benzoyl methyl ester benzoic acid, 2,9-dimethyl decane, and pentadecane were identified from the urine of dengue patients. The identified secretion of alkane may be a suitable candidate for colorimetric assay for the development of a user-friendly, home-screening rapid test kit for the detection of dengue infection.
format	Article
author	Amid, Azura Abdullah Sani, Muhamad Shirwan Abdullah, Aszrin Mohamed Bakrim, Norbaiyah Wan Omar, Wan Fatein Nabeila Zakaria, Nurul Aulia Abdullah, Nor Zamzila Nik Ahmad, Nik Nur Fatnoon
author_facet	Amid, Azura Abdullah Sani, Muhamad Shirwan Abdullah, Aszrin Mohamed Bakrim, Norbaiyah Wan Omar, Wan Fatein Nabeila Zakaria, Nurul Aulia Abdullah, Nor Zamzila Nik Ahmad, Nik Nur Fatnoon
author_sort	Amid, Azura
title	Biomarkers identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
title_short	Biomarkers identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
title_full	Biomarkers identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
title_fullStr	Biomarkers identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
title_full_unstemmed	Biomarkers identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
title_sort	biomarkers identification from urine of dengue patients through gcms for initial development of non-invasive diagnostic kit
publisher	IIUM Press
publishDate	2024
url	http://irep.iium.edu.my/113961/2/113961_Biomarkers%20identification%20from%20urine.pdf http://irep.iium.edu.my/113961/3/113961_Biomarkers%20identification%20from%20urine_Scopus.pdf http://irep.iium.edu.my/113961/ https://journals.iium.edu.my/ejournal/index.php/iiumej https://doi.org/10.31436/iiumej.v25i2.2785
_version_	1809136366754201600
score	13.211869

Biomarkers identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit

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