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Allergenic potential and cross-reactivity of fungal species isolated from the indoor environment

Indoor fungi are potential sensitizing agents. Their detection and quantification in indoor environments are important in the diagnosis and environmental management of fungal allergies. This study aims to analyse the allergenic potential of ten fungal species and the cross-reactivity of the two most...

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Main Authors: Abdullah, Mai Shihah, Kttafah, Ghassan Hadi, Nasuruddin, Muhammad Haidar
Format: Article
Language:English
English
Published: Penerbit UTM Press 2022
Subjects:
R Medicine (General)
RA0421 Public health. Hygiene. Preventive Medicine
Online Access:http://irep.iium.edu.my/113834/2/113834_%20Allergenic%20potential%20and%20cross-reactivity.pdf
http://irep.iium.edu.my/113834/3/113834_%20Allergenic%20potential%20and%20cross-reactivity_Scopus.pdf
http://irep.iium.edu.my/113834/
https://journals.utm.my/index.php/jurnalteknologi
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spelling my.iium.irep.1138342024-08-12T04:50:58Z http://irep.iium.edu.my/113834/ Allergenic potential and cross-reactivity of fungal species isolated from the indoor environment Abdullah, Mai Shihah Kttafah, Ghassan Hadi Nasuruddin, Muhammad Haidar R Medicine (General) RA0421 Public health. Hygiene. Preventive Medicine Indoor fungi are potential sensitizing agents. Their detection and quantification in indoor environments are important in the diagnosis and environmental management of fungal allergies. This study aims to analyse the allergenic potential of ten fungal species and the cross-reactivity of the two most common fungi isolated from the indoor environment samples from Sultan Idris Education University buildings. Employing in vivo (skin prick test) and in vitro (immunoblotting), the major and minor allergenic proteins of ten fungi sensitized subjects were identified. Aspergillus fumigatus and Penicillium canescens were the most common fungi with the highest potential to trigger allergies. The cross-reactivity between them was detected by immunoblotting inhibition experiments using three selected sera from subjects sensitized to each of the aforementioned species. The immunoblotting test revealed multiple major and minor allergens. Among them were 11, 25, 33, 36 > 100 kDa and were also listed as causative agent triggering allergy by IUIS Allergen Nomenclature Subcommittee. Cross-reactivity of Aspergillus fumigatus against Penicillium canescens revealed that 9(64.29%) allergenic bands and 13(76.47%) allergenic bands were inhibited, respectively. Aspergillus spp. and Penicillium spp. with high cross-reactivity are most prevalent in the indoor environment of identified contaminated buildings at UPSI. Aspergillus fumigatus and Penicillium canescens can elicit sensitization among the atopic population and implicates worsening the condition of the symptomatic subjects with prolonged these fungal exposures. Penerbit UTM Press 2022-04-20 Article PeerReviewed application/pdf en http://irep.iium.edu.my/113834/2/113834_%20Allergenic%20potential%20and%20cross-reactivity.pdf application/pdf en http://irep.iium.edu.my/113834/3/113834_%20Allergenic%20potential%20and%20cross-reactivity_Scopus.pdf Abdullah, Mai Shihah and Kttafah, Ghassan Hadi and Nasuruddin, Muhammad Haidar (2022) Allergenic potential and cross-reactivity of fungal species isolated from the indoor environment. Jurnal Teknologi, 84 (3). pp. 47-57. E-ISSN 2180-3722 https://journals.utm.my/index.php/jurnalteknologi Penerbit UTM Press
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic R Medicine (General)
RA0421 Public health. Hygiene. Preventive Medicine
spellingShingle R Medicine (General)
RA0421 Public health. Hygiene. Preventive Medicine
Abdullah, Mai Shihah
Kttafah, Ghassan Hadi
Nasuruddin, Muhammad Haidar
Allergenic potential and cross-reactivity of fungal species isolated from the indoor environment
description Indoor fungi are potential sensitizing agents. Their detection and quantification in indoor environments are important in the diagnosis and environmental management of fungal allergies. This study aims to analyse the allergenic potential of ten fungal species and the cross-reactivity of the two most common fungi isolated from the indoor environment samples from Sultan Idris Education University buildings. Employing in vivo (skin prick test) and in vitro (immunoblotting), the major and minor allergenic proteins of ten fungi sensitized subjects were identified. Aspergillus fumigatus and Penicillium canescens were the most common fungi with the highest potential to trigger allergies. The cross-reactivity between them was detected by immunoblotting inhibition experiments using three selected sera from subjects sensitized to each of the aforementioned species. The immunoblotting test revealed multiple major and minor allergens. Among them were 11, 25, 33, 36 > 100 kDa and were also listed as causative agent triggering allergy by IUIS Allergen Nomenclature Subcommittee. Cross-reactivity of Aspergillus fumigatus against Penicillium canescens revealed that 9(64.29%) allergenic bands and 13(76.47%) allergenic bands were inhibited, respectively. Aspergillus spp. and Penicillium spp. with high cross-reactivity are most prevalent in the indoor environment of identified contaminated buildings at UPSI. Aspergillus fumigatus and Penicillium canescens can elicit sensitization among the atopic population and implicates worsening the condition of the symptomatic subjects with prolonged these fungal exposures.
format Article
author Abdullah, Mai Shihah
Kttafah, Ghassan Hadi
Nasuruddin, Muhammad Haidar
author_facet Abdullah, Mai Shihah
Kttafah, Ghassan Hadi
Nasuruddin, Muhammad Haidar
author_sort Abdullah, Mai Shihah
title Allergenic potential and cross-reactivity of fungal species isolated from the indoor environment
title_short Allergenic potential and cross-reactivity of fungal species isolated from the indoor environment
title_full Allergenic potential and cross-reactivity of fungal species isolated from the indoor environment
title_fullStr Allergenic potential and cross-reactivity of fungal species isolated from the indoor environment
title_full_unstemmed Allergenic potential and cross-reactivity of fungal species isolated from the indoor environment
title_sort allergenic potential and cross-reactivity of fungal species isolated from the indoor environment
publisher Penerbit UTM Press
publishDate 2022
url http://irep.iium.edu.my/113834/2/113834_%20Allergenic%20potential%20and%20cross-reactivity.pdf
http://irep.iium.edu.my/113834/3/113834_%20Allergenic%20potential%20and%20cross-reactivity_Scopus.pdf
http://irep.iium.edu.my/113834/
https://journals.utm.my/index.php/jurnalteknologi
_version_ 1809136356835721216
score 13.226497

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