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Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: in vitro analysis and molecular docking study

Antioxidant activity and antihyperglycemic constituents and of traditional medicinal plants are currently the preferred therapeutic means of treatment and management of diabetes because of the undesired adverse effect of synthetic drugs. Muntingia calabura (Kerukupsiam) leaves and other parts are...

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Main Authors:	Nagoor Gunny, Ahmad Anas, K Prammakumar, Nimaal, Ahmad, Abdul Aziz, C.B. Gopinath, Subash, Abu Bakar, Amirul Ridzuan, Musa, Haliru, Che Mat, Mohd Hishamuddin
Format:	Article
Language:	English English English
Published:	Elsevier 2024
Subjects:	Q Science (General) QD Chemistry QR Microbiology
Online Access:	http://irep.iium.edu.my/113186/1/113186_Exploring%20antioxidant.pdf http://irep.iium.edu.my/113186/2/113186_Exploring%20antioxidant_SCOPUS.pdf http://irep.iium.edu.my/113186/9/113186_Exploring%20antioxidant_WOS.pdf http://irep.iium.edu.my/113186/ https://www.sciencedirect.com/science/article/pii/S2211715624000018?via%3Dihub
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spelling	my.iium.irep.1131862024-07-18T09:20:28Z http://irep.iium.edu.my/113186/ Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: in vitro analysis and molecular docking study Nagoor Gunny, Ahmad Anas K Prammakumar, Nimaal Ahmad, Abdul Aziz C.B. Gopinath, Subash Abu Bakar, Amirul Ridzuan Musa, Haliru Che Mat, Mohd Hishamuddin Q Science (General) QD Chemistry QR Microbiology Antioxidant activity and antihyperglycemic constituents and of traditional medicinal plants are currently the preferred therapeutic means of treatment and management of diabetes because of the undesired adverse effect of synthetic drugs. Muntingia calabura (Kerukupsiam) leaves and other parts are considered as alternative natural sources of treatment for diabetes. Ultrasonic assisted extraction is a novel approach for extraction of phytoconstituents which gives high extraction yield of bioactive compounds. However, there has been no published information presently on the use of ethanol ultrasonic assisted extraction method for assessment of antioxidant and antidiabetic activities of M. calabura leaves. Hence, the current study aims to evaluate the in vitro antioxidant and antidiabetic activities of M. calabura leave extract. IC50 analysis was done to determine theinhibitory concentration and the results obtained from 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay showed IC50 of gallic acid to be 1.0 µg/ml, which is lower than M. calabura leaves extract at 2.54 µg/ml, indicating that only small concentration of gallic acid was required to inhibit the free radicals at 50 %. However, IC50 analysis for amylase inhibition showed that M. calabura extract had 44.39 µg/ml antidiabetic activity compared to acarbose with 57.1 µg/ml activity. This indicates that M. calabura leaves extract has a better inhibition on amylase activity compared to the acarbose which is a synthetic drug. Further still, in silico study was carried out and the molecular docking result of eight ligands against amylase indicates quercetin had the least binding free energy of − 9.1 kcal/mol, indicating the strongest interaction. Using Lineweaver-Burk plot, the results showed a competitive inhibition, hence, it was justified that M. calabura has the potential to manage diabetes and other diseases related to free radicals. Elsevier 2024-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/113186/1/113186_Exploring%20antioxidant.pdf application/pdf en http://irep.iium.edu.my/113186/2/113186_Exploring%20antioxidant_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/113186/9/113186_Exploring%20antioxidant_WOS.pdf Nagoor Gunny, Ahmad Anas and K Prammakumar, Nimaal and Ahmad, Abdul Aziz and C.B. Gopinath, Subash and Abu Bakar, Amirul Ridzuan and Musa, Haliru and Che Mat, Mohd Hishamuddin (2024) Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: in vitro analysis and molecular docking study. Results in Chemistry, 7. pp. 1-9. E-ISSN 2211-7156 https://www.sciencedirect.com/science/article/pii/S2211715624000018?via%3Dihub 10.1016/j.rechem.2024.101305
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English English
topic	Q Science (General) QD Chemistry QR Microbiology
spellingShingle	Q Science (General) QD Chemistry QR Microbiology Nagoor Gunny, Ahmad Anas K Prammakumar, Nimaal Ahmad, Abdul Aziz C.B. Gopinath, Subash Abu Bakar, Amirul Ridzuan Musa, Haliru Che Mat, Mohd Hishamuddin Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: in vitro analysis and molecular docking study
description	Antioxidant activity and antihyperglycemic constituents and of traditional medicinal plants are currently the preferred therapeutic means of treatment and management of diabetes because of the undesired adverse effect of synthetic drugs. Muntingia calabura (Kerukupsiam) leaves and other parts are considered as alternative natural sources of treatment for diabetes. Ultrasonic assisted extraction is a novel approach for extraction of phytoconstituents which gives high extraction yield of bioactive compounds. However, there has been no published information presently on the use of ethanol ultrasonic assisted extraction method for assessment of antioxidant and antidiabetic activities of M. calabura leaves. Hence, the current study aims to evaluate the in vitro antioxidant and antidiabetic activities of M. calabura leave extract. IC50 analysis was done to determine theinhibitory concentration and the results obtained from 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay showed IC50 of gallic acid to be 1.0 µg/ml, which is lower than M. calabura leaves extract at 2.54 µg/ml, indicating that only small concentration of gallic acid was required to inhibit the free radicals at 50 %. However, IC50 analysis for amylase inhibition showed that M. calabura extract had 44.39 µg/ml antidiabetic activity compared to acarbose with 57.1 µg/ml activity. This indicates that M. calabura leaves extract has a better inhibition on amylase activity compared to the acarbose which is a synthetic drug. Further still, in silico study was carried out and the molecular docking result of eight ligands against amylase indicates quercetin had the least binding free energy of − 9.1 kcal/mol, indicating the strongest interaction. Using Lineweaver-Burk plot, the results showed a competitive inhibition, hence, it was justified that M. calabura has the potential to manage diabetes and other diseases related to free radicals.
format	Article
author	Nagoor Gunny, Ahmad Anas K Prammakumar, Nimaal Ahmad, Abdul Aziz C.B. Gopinath, Subash Abu Bakar, Amirul Ridzuan Musa, Haliru Che Mat, Mohd Hishamuddin
author_facet	Nagoor Gunny, Ahmad Anas K Prammakumar, Nimaal Ahmad, Abdul Aziz C.B. Gopinath, Subash Abu Bakar, Amirul Ridzuan Musa, Haliru Che Mat, Mohd Hishamuddin
author_sort	Nagoor Gunny, Ahmad Anas
title	Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: in vitro analysis and molecular docking study
title_short	Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: in vitro analysis and molecular docking study
title_full	Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: in vitro analysis and molecular docking study
title_fullStr	Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: in vitro analysis and molecular docking study
title_full_unstemmed	Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: in vitro analysis and molecular docking study
title_sort	exploring antioxidant and antidiabetic potential of mutingia calabura (kerukupsiam) leaf extract: in vitro analysis and molecular docking study
publisher	Elsevier
publishDate	2024
url	http://irep.iium.edu.my/113186/1/113186_Exploring%20antioxidant.pdf http://irep.iium.edu.my/113186/2/113186_Exploring%20antioxidant_SCOPUS.pdf http://irep.iium.edu.my/113186/9/113186_Exploring%20antioxidant_WOS.pdf http://irep.iium.edu.my/113186/ https://www.sciencedirect.com/science/article/pii/S2211715624000018?via%3Dihub
_version_	1805880574246649856
score	13.211869

Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: in vitro analysis and molecular docking study

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