Staff View: Pathogenicity of betanodavirus strains in Asian seabass (Lates Calcarifer) under temperature fluctuation stress

Pathogenicity of betanodavirus strains in Asian seabass (Lates Calcarifer) under temperature fluctuation stress

Viral nervous necrosis (VNN) caused by betanodavirus is responsible for mass mortality in various marine cultured fish species. VNN can be transmitted horizontally and vertically. In this study, three betanodavirus strains (BE2006, AVA, and KL0022) were obtained from National Fish Health Research Ce...

Full description

Saved in:

Bibliographic Details
Main Authors:	Khairul Asri, Muhammad Safwan, Abdullah, Azila, Nawi, Mohd Firdaus, Mohd Idris, Shaharah, Ramly, Rimatulhana, Mansor, Nur Nazifah
Format:	Article
Language:	English
Published:	Fisheries Research Institute (FRI), Department of Fisheries Malaysia 2024
Subjects:	Q Science (General) QR Microbiology QR355 Virology S494.5.S86 Sustainable agriculture SH151 Aquaculture - Fish Culture
Online Access:	http://irep.iium.edu.my/113144/1/113144_Pathogenicity%20of%20betanodavirus%20strains.pdf http://irep.iium.edu.my/113144/ https://fri.dof.gov.my/en/publications-malaysia-fisheries-journal-mfj/
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.iium.irep.113144
record_format	dspace
spelling	my.iium.irep.1131442024-07-15T07:36:04Z http://irep.iium.edu.my/113144/ Pathogenicity of betanodavirus strains in Asian seabass (Lates Calcarifer) under temperature fluctuation stress Khairul Asri, Muhammad Safwan Abdullah, Azila Nawi, Mohd Firdaus Mohd Idris, Shaharah Ramly, Rimatulhana Mansor, Nur Nazifah Q Science (General) QR Microbiology QR355 Virology S494.5.S86 Sustainable agriculture SH151 Aquaculture - Fish Culture Viral nervous necrosis (VNN) caused by betanodavirus is responsible for mass mortality in various marine cultured fish species. VNN can be transmitted horizontally and vertically. In this study, three betanodavirus strains (BE2006, AVA, and KL0022) were obtained from National Fish Health Research Centre (NaFisH) and cultured in the E-11 cell line. The median tissue culture infective dose (TCID50) for all isolates was determined before being used in in vivo experiments. The TCID50 of KL0022, BE2006, and AVA were 104 TCID50/mL, 107 TCID50/mL, and 106 TCID50/ mL, respectively. The KL0022 strain had the lowest TCID50, followed by that of AVA and BE2006. Subsequently, 360 healthy juvenile Asian seabass (5 ± 2 g) were divided equally into eight tanks to represent four treatment groups with duplicates: Group 1 (AVA), Group 2 (BE2006), Group 3 (KL0022), and Group 4 (negative control). The fish were observed daily for clinical signs or mortality. On the appearance of clinical signs or mortality, the fish were killed immediately and underwent a reverse transcriptase–PCR (RT-PCR) and virus cell culture. The experiment was terminated after 30 days. The clinical signs observed among the sick and dead fish were darker body colouration and lethargy. The highest mortality was recorded in the KL0022 group, indicating that the strain had the highest virulence, followed by the AVA and BE2006 groups. All dead and sick fish from the treatment groups were positive for betanodavirus by RT-PCR, while the dead fish from the negative control group tested negative. Fisheries Research Institute (FRI), Department of Fisheries Malaysia 2024 Article PeerReviewed application/pdf en http://irep.iium.edu.my/113144/1/113144_Pathogenicity%20of%20betanodavirus%20strains.pdf Khairul Asri, Muhammad Safwan and Abdullah, Azila and Nawi, Mohd Firdaus and Mohd Idris, Shaharah and Ramly, Rimatulhana and Mansor, Nur Nazifah (2024) Pathogenicity of betanodavirus strains in Asian seabass (Lates Calcarifer) under temperature fluctuation stress. Malaysian Fisheries Journal, 24 (Special Issue 2). pp. 45-53. ISSN 1511-7286 https://fri.dof.gov.my/en/publications-malaysia-fisheries-journal-mfj/
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	Q Science (General) QR Microbiology QR355 Virology S494.5.S86 Sustainable agriculture SH151 Aquaculture - Fish Culture
spellingShingle	Q Science (General) QR Microbiology QR355 Virology S494.5.S86 Sustainable agriculture SH151 Aquaculture - Fish Culture Khairul Asri, Muhammad Safwan Abdullah, Azila Nawi, Mohd Firdaus Mohd Idris, Shaharah Ramly, Rimatulhana Mansor, Nur Nazifah Pathogenicity of betanodavirus strains in Asian seabass (Lates Calcarifer) under temperature fluctuation stress
description	Viral nervous necrosis (VNN) caused by betanodavirus is responsible for mass mortality in various marine cultured fish species. VNN can be transmitted horizontally and vertically. In this study, three betanodavirus strains (BE2006, AVA, and KL0022) were obtained from National Fish Health Research Centre (NaFisH) and cultured in the E-11 cell line. The median tissue culture infective dose (TCID50) for all isolates was determined before being used in in vivo experiments. The TCID50 of KL0022, BE2006, and AVA were 104 TCID50/mL, 107 TCID50/mL, and 106 TCID50/ mL, respectively. The KL0022 strain had the lowest TCID50, followed by that of AVA and BE2006. Subsequently, 360 healthy juvenile Asian seabass (5 ± 2 g) were divided equally into eight tanks to represent four treatment groups with duplicates: Group 1 (AVA), Group 2 (BE2006), Group 3 (KL0022), and Group 4 (negative control). The fish were observed daily for clinical signs or mortality. On the appearance of clinical signs or mortality, the fish were killed immediately and underwent a reverse transcriptase–PCR (RT-PCR) and virus cell culture. The experiment was terminated after 30 days. The clinical signs observed among the sick and dead fish were darker body colouration and lethargy. The highest mortality was recorded in the KL0022 group, indicating that the strain had the highest virulence, followed by the AVA and BE2006 groups. All dead and sick fish from the treatment groups were positive for betanodavirus by RT-PCR, while the dead fish from the negative control group tested negative.
format	Article
author	Khairul Asri, Muhammad Safwan Abdullah, Azila Nawi, Mohd Firdaus Mohd Idris, Shaharah Ramly, Rimatulhana Mansor, Nur Nazifah
author_facet	Khairul Asri, Muhammad Safwan Abdullah, Azila Nawi, Mohd Firdaus Mohd Idris, Shaharah Ramly, Rimatulhana Mansor, Nur Nazifah
author_sort	Khairul Asri, Muhammad Safwan
title	Pathogenicity of betanodavirus strains in Asian seabass (Lates Calcarifer) under temperature fluctuation stress
title_short	Pathogenicity of betanodavirus strains in Asian seabass (Lates Calcarifer) under temperature fluctuation stress
title_full	Pathogenicity of betanodavirus strains in Asian seabass (Lates Calcarifer) under temperature fluctuation stress
title_fullStr	Pathogenicity of betanodavirus strains in Asian seabass (Lates Calcarifer) under temperature fluctuation stress
title_full_unstemmed	Pathogenicity of betanodavirus strains in Asian seabass (Lates Calcarifer) under temperature fluctuation stress
title_sort	pathogenicity of betanodavirus strains in asian seabass (lates calcarifer) under temperature fluctuation stress
publisher	Fisheries Research Institute (FRI), Department of Fisheries Malaysia
publishDate	2024
url	http://irep.iium.edu.my/113144/1/113144_Pathogenicity%20of%20betanodavirus%20strains.pdf http://irep.iium.edu.my/113144/ https://fri.dof.gov.my/en/publications-malaysia-fisheries-journal-mfj/
_version_	1805880565423931392
score	13.211869

Pathogenicity of betanodavirus strains in Asian seabass (Lates Calcarifer) under temperature fluctuation stress

Similar Items