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Genotypic and phenotypic characterisation of isolated marine bacteria and its potential to produce alkaline protease

In several industries such as food and detergent, enzymes are commonly used out of which microorganisms' proteolytic enzymes are dominant. Microorganisms from marine sources have advantageous commercial characteristics when it comes to protease production. Protease producing bacterial strains w...

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Main Authors: Dehimi, Maya, Yusof, Faridah, Raus, Raha Bt. Ahmad, Nordin, Noor Faizul Hadry, Nedjai, Radhia
Format: Article
Language:English
English
Published: Akademia Baru Publishing (M) Sdn Bhd 2021
Subjects:
QR Microbiology
Online Access:http://irep.iium.edu.my/113040/2/113040_%20Genotypic%20and%20phenotypic%20characterisation.pdf
http://irep.iium.edu.my/113040/3/113040_%20Genotypic%20and%20phenotypic%20characterisation_Scopus.pdf
http://irep.iium.edu.my/113040/
https://akademiabaru.com/submit/index.php/araset/article/view/3411
https://doi.org/10.37934/araset.22.1.1625
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spelling my.iium.irep.1130402024-07-10T01:29:12Z http://irep.iium.edu.my/113040/ Genotypic and phenotypic characterisation of isolated marine bacteria and its potential to produce alkaline protease Dehimi, Maya Yusof, Faridah Raus, Raha Bt. Ahmad Nordin, Noor Faizul Hadry Nedjai, Radhia QR Microbiology In several industries such as food and detergent, enzymes are commonly used out of which microorganisms' proteolytic enzymes are dominant. Microorganisms from marine sources have advantageous commercial characteristics when it comes to protease production. Protease producing bacterial strains were isolated from four different stations in the South China Sea, located at Kuantan, Pahang-Malaysia. The temperature was ranging from 29 to 36.2°C; salinity varied from 36,18 to 37,9 ppt, turbidity from 10.21 to 16.4 NTU. Dissolved Oxygen had an average of 4.44 mg/mL, and pH was around 8. All the bacterial strains were screened for protease activity using Skim Milk agar plates. Out of 18 isolates, ten strains formed a clear zone on skim milk agar plates. Molecular identification by 16S rRNA results revealed that Bacillus spp was the most dominant bacteria, four isolates belong to Bacillus cereus, another 04 identified as Bacillus licheniformis in addition of one isolate was Bacillus safensis. They were followed by one isolate Staphylococcus warneri. Furthermore, a phenotypic characterization is conducted using The Biolog GENIII MicroPlate with 94 phenotypic tests. These tests consist of 71 carbon source utilization assays and 23 chemical sensitivity assays. The proteolytic activity was confirmed through a quantitative protease assay. Where the strain B. cereus-MD152 was the highest yield, followed by B. licheniformis-ABN13. On the contrary, the lowest yield was secreted by B. cereus-IIUM6 with no more than 16.5 U/ml. Akademia Baru Publishing (M) Sdn Bhd 2021-01-28 Article PeerReviewed application/pdf en http://irep.iium.edu.my/113040/2/113040_%20Genotypic%20and%20phenotypic%20characterisation.pdf application/pdf en http://irep.iium.edu.my/113040/3/113040_%20Genotypic%20and%20phenotypic%20characterisation_Scopus.pdf Dehimi, Maya and Yusof, Faridah and Raus, Raha Bt. Ahmad and Nordin, Noor Faizul Hadry and Nedjai, Radhia (2021) Genotypic and phenotypic characterisation of isolated marine bacteria and its potential to produce alkaline protease. Journal of Advanced Research in Applied Sciences and Engineering Technology, 22 (1). pp. 16-25. ISSN 2462-1943 https://akademiabaru.com/submit/index.php/araset/article/view/3411 https://doi.org/10.37934/araset.22.1.1625
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QR Microbiology
spellingShingle QR Microbiology
Dehimi, Maya
Yusof, Faridah
Raus, Raha Bt. Ahmad
Nordin, Noor Faizul Hadry
Nedjai, Radhia
Genotypic and phenotypic characterisation of isolated marine bacteria and its potential to produce alkaline protease
description In several industries such as food and detergent, enzymes are commonly used out of which microorganisms' proteolytic enzymes are dominant. Microorganisms from marine sources have advantageous commercial characteristics when it comes to protease production. Protease producing bacterial strains were isolated from four different stations in the South China Sea, located at Kuantan, Pahang-Malaysia. The temperature was ranging from 29 to 36.2°C; salinity varied from 36,18 to 37,9 ppt, turbidity from 10.21 to 16.4 NTU. Dissolved Oxygen had an average of 4.44 mg/mL, and pH was around 8. All the bacterial strains were screened for protease activity using Skim Milk agar plates. Out of 18 isolates, ten strains formed a clear zone on skim milk agar plates. Molecular identification by 16S rRNA results revealed that Bacillus spp was the most dominant bacteria, four isolates belong to Bacillus cereus, another 04 identified as Bacillus licheniformis in addition of one isolate was Bacillus safensis. They were followed by one isolate Staphylococcus warneri. Furthermore, a phenotypic characterization is conducted using The Biolog GENIII MicroPlate with 94 phenotypic tests. These tests consist of 71 carbon source utilization assays and 23 chemical sensitivity assays. The proteolytic activity was confirmed through a quantitative protease assay. Where the strain B. cereus-MD152 was the highest yield, followed by B. licheniformis-ABN13. On the contrary, the lowest yield was secreted by B. cereus-IIUM6 with no more than 16.5 U/ml.
format Article
author Dehimi, Maya
Yusof, Faridah
Raus, Raha Bt. Ahmad
Nordin, Noor Faizul Hadry
Nedjai, Radhia
author_facet Dehimi, Maya
Yusof, Faridah
Raus, Raha Bt. Ahmad
Nordin, Noor Faizul Hadry
Nedjai, Radhia
author_sort Dehimi, Maya
title Genotypic and phenotypic characterisation of isolated marine bacteria and its potential to produce alkaline protease
title_short Genotypic and phenotypic characterisation of isolated marine bacteria and its potential to produce alkaline protease
title_full Genotypic and phenotypic characterisation of isolated marine bacteria and its potential to produce alkaline protease
title_fullStr Genotypic and phenotypic characterisation of isolated marine bacteria and its potential to produce alkaline protease
title_full_unstemmed Genotypic and phenotypic characterisation of isolated marine bacteria and its potential to produce alkaline protease
title_sort genotypic and phenotypic characterisation of isolated marine bacteria and its potential to produce alkaline protease
publisher Akademia Baru Publishing (M) Sdn Bhd
publishDate 2021
url http://irep.iium.edu.my/113040/2/113040_%20Genotypic%20and%20phenotypic%20characterisation.pdf
http://irep.iium.edu.my/113040/3/113040_%20Genotypic%20and%20phenotypic%20characterisation_Scopus.pdf
http://irep.iium.edu.my/113040/
https://akademiabaru.com/submit/index.php/araset/article/view/3411
https://doi.org/10.37934/araset.22.1.1625
_version_ 1805880555806392320
score 13.211869

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