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Effect of Matricaria aurea essential oils on biofilm development, virulence factors and quorum sensing-dependent genes of Pseudomonas aeruginosa

Abstract: The emergence of drug-resistant microorganisms presents a substantial global public health threat. The increase in pathogens resistant to commonly prescribed antibiotics underscores the urgent requirement to explore alternative treatment strategies. This study adopts a novel approach by ha...

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Main Authors: Qaralleh, Haitham, Mohammed Saghir, Sultan Ayesh, Al-limoun, Muhamad Odeh Atyeh, M. Dmor, Saif, Khleifat, Khaled, Mustafa Al-Ahmad, Basma Ezzat, Al-Omari, Laila, Tabana, Yasser, Mothana, Ramzi Ahmed, M. Al-Yousef, Hanan, M. Alqahtani, Abdulaziz
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute (MDPI) 2024
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/111654/7/111654_Effect%20of%20Matricaria%20Aurea%20essential%20oils%20on%20biofilm%20development.pdf
http://irep.iium.edu.my/111654/
https://www.mdpi.com/1424-8247/17/3/386
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spelling my.iium.irep.1116542024-03-27T06:37:31Z http://irep.iium.edu.my/111654/ Effect of Matricaria aurea essential oils on biofilm development, virulence factors and quorum sensing-dependent genes of Pseudomonas aeruginosa Qaralleh, Haitham Mohammed Saghir, Sultan Ayesh Al-limoun, Muhamad Odeh Atyeh M. Dmor, Saif Khleifat, Khaled Mustafa Al-Ahmad, Basma Ezzat Al-Omari, Laila Tabana, Yasser Mothana, Ramzi Ahmed M. Al-Yousef, Hanan M. Alqahtani, Abdulaziz R Medicine (General) Abstract: The emergence of drug-resistant microorganisms presents a substantial global public health threat. The increase in pathogens resistant to commonly prescribed antibiotics underscores the urgent requirement to explore alternative treatment strategies. This study adopts a novel approach by harnessing natural resources, specifically essential oils (EO), to combat bacterial pathogenicity. The primary aim of this research was to analyze the chemical composition of the aerial part of the Matricaria aurea (M. aureas) EO and evaluate its potential for inhibiting quorum sensing (QS) and disrupting biofilm formation in Pseudomonas aeruginosa (P. aeruginosa). The gas chromatography-mass spectrometry (GCMS) analysis unveiled that α-bisabolol oxide A constituted the predominant portion, comprising 64.8% of the total, with β-bisabolene at 6.3% and α-farnesene at 4.8% following closely behind. The antibiofilm efficacy was observed at concentrations of 0.3, 0.15, and 0.08 mg/mL, demonstrating negligible effects on cell viability. Furthermore, the EO from M. aurea effectively inhibited the formation of P. aeruginosa biofilms by diminishing aggregation, hydrophobicity, and swarming motility. Significantly, the EO treatment resulted in a conspicuous decrease in the production of pyocyanin, rhamnolipid, and extracellular polymeric substances (EPS), along with a reduction in the enzymatic activity of protease and chitinase. The EO effectively hindered QS by disrupting QS mechanisms, resulting in a marked decline in the secretion of N-Acyl homoserine lactone (AHL) molecules and the expression of phazA1 and aprA genes. This investigation offers compelling evidence supporting the potential of M. aurea EO as a promising therapeutic candidate for addressing infectious diseases induced by biofilm formation. Multidisciplinary Digital Publishing Institute (MDPI) 2024-03-18 Article PeerReviewed application/pdf en http://irep.iium.edu.my/111654/7/111654_Effect%20of%20Matricaria%20Aurea%20essential%20oils%20on%20biofilm%20development.pdf Qaralleh, Haitham and Mohammed Saghir, Sultan Ayesh and Al-limoun, Muhamad Odeh Atyeh and M. Dmor, Saif and Khleifat, Khaled and Mustafa Al-Ahmad, Basma Ezzat and Al-Omari, Laila and Tabana, Yasser and Mothana, Ramzi Ahmed and M. Al-Yousef, Hanan and M. Alqahtani, Abdulaziz (2024) Effect of Matricaria aurea essential oils on biofilm development, virulence factors and quorum sensing-dependent genes of Pseudomonas aeruginosa. Pharmaceuticals, 17 (3). ISSN 1424-8247 https://www.mdpi.com/1424-8247/17/3/386 10.3390/ph17030386
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Qaralleh, Haitham
Mohammed Saghir, Sultan Ayesh
Al-limoun, Muhamad Odeh Atyeh
M. Dmor, Saif
Khleifat, Khaled
Mustafa Al-Ahmad, Basma Ezzat
Al-Omari, Laila
Tabana, Yasser
Mothana, Ramzi Ahmed
M. Al-Yousef, Hanan
M. Alqahtani, Abdulaziz
Effect of Matricaria aurea essential oils on biofilm development, virulence factors and quorum sensing-dependent genes of Pseudomonas aeruginosa
description Abstract: The emergence of drug-resistant microorganisms presents a substantial global public health threat. The increase in pathogens resistant to commonly prescribed antibiotics underscores the urgent requirement to explore alternative treatment strategies. This study adopts a novel approach by harnessing natural resources, specifically essential oils (EO), to combat bacterial pathogenicity. The primary aim of this research was to analyze the chemical composition of the aerial part of the Matricaria aurea (M. aureas) EO and evaluate its potential for inhibiting quorum sensing (QS) and disrupting biofilm formation in Pseudomonas aeruginosa (P. aeruginosa). The gas chromatography-mass spectrometry (GCMS) analysis unveiled that α-bisabolol oxide A constituted the predominant portion, comprising 64.8% of the total, with β-bisabolene at 6.3% and α-farnesene at 4.8% following closely behind. The antibiofilm efficacy was observed at concentrations of 0.3, 0.15, and 0.08 mg/mL, demonstrating negligible effects on cell viability. Furthermore, the EO from M. aurea effectively inhibited the formation of P. aeruginosa biofilms by diminishing aggregation, hydrophobicity, and swarming motility. Significantly, the EO treatment resulted in a conspicuous decrease in the production of pyocyanin, rhamnolipid, and extracellular polymeric substances (EPS), along with a reduction in the enzymatic activity of protease and chitinase. The EO effectively hindered QS by disrupting QS mechanisms, resulting in a marked decline in the secretion of N-Acyl homoserine lactone (AHL) molecules and the expression of phazA1 and aprA genes. This investigation offers compelling evidence supporting the potential of M. aurea EO as a promising therapeutic candidate for addressing infectious diseases induced by biofilm formation.
format Article
author Qaralleh, Haitham
Mohammed Saghir, Sultan Ayesh
Al-limoun, Muhamad Odeh Atyeh
M. Dmor, Saif
Khleifat, Khaled
Mustafa Al-Ahmad, Basma Ezzat
Al-Omari, Laila
Tabana, Yasser
Mothana, Ramzi Ahmed
M. Al-Yousef, Hanan
M. Alqahtani, Abdulaziz
author_facet Qaralleh, Haitham
Mohammed Saghir, Sultan Ayesh
Al-limoun, Muhamad Odeh Atyeh
M. Dmor, Saif
Khleifat, Khaled
Mustafa Al-Ahmad, Basma Ezzat
Al-Omari, Laila
Tabana, Yasser
Mothana, Ramzi Ahmed
M. Al-Yousef, Hanan
M. Alqahtani, Abdulaziz
author_sort Qaralleh, Haitham
title Effect of Matricaria aurea essential oils on biofilm development, virulence factors and quorum sensing-dependent genes of Pseudomonas aeruginosa
title_short Effect of Matricaria aurea essential oils on biofilm development, virulence factors and quorum sensing-dependent genes of Pseudomonas aeruginosa
title_full Effect of Matricaria aurea essential oils on biofilm development, virulence factors and quorum sensing-dependent genes of Pseudomonas aeruginosa
title_fullStr Effect of Matricaria aurea essential oils on biofilm development, virulence factors and quorum sensing-dependent genes of Pseudomonas aeruginosa
title_full_unstemmed Effect of Matricaria aurea essential oils on biofilm development, virulence factors and quorum sensing-dependent genes of Pseudomonas aeruginosa
title_sort effect of matricaria aurea essential oils on biofilm development, virulence factors and quorum sensing-dependent genes of pseudomonas aeruginosa
publisher Multidisciplinary Digital Publishing Institute (MDPI)
publishDate 2024
url http://irep.iium.edu.my/111654/7/111654_Effect%20of%20Matricaria%20Aurea%20essential%20oils%20on%20biofilm%20development.pdf
http://irep.iium.edu.my/111654/
https://www.mdpi.com/1424-8247/17/3/386
_version_ 1795008253544890368
score 13.211869

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