Staff View: Baseline amplicon sequencing data for the ITS2 region in the green honey of Banggi Island, Sabah

Baseline amplicon sequencing data for the ITS2 region in the green honey of Banggi Island, Sabah

Green honey, was discovered on Banggi Island, Sabah, showing high in essential amino acids and chlorophyll derivatives. Despite its lucrative market potential owing to its distinctive color, uncertainties persist regarding its nature. This study leverages amplicon sequencing by targeting micro- and...

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Main Authors:	Ullah, Saeed, Huda, Nurul, Ab Wahab, Roswanira, Abdul Hamid, Azzmer Azzar, Mohd Nasir, Mohd Hamzah, Mohamad, Mohd Azrul Naim, Ahmad, Hajar Fauzan, Oyewusi, Habeebat Adekilekun, Huyop, Fahrul
Format:	Article
Language:	English English
Published:	Elsevier 2024
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/110947/1/110947_Baseline%20amplicon%20sequencing%20data.pdf http://irep.iium.edu.my/110947/2/110947_Baseline%20amplicon%20sequencing%20data_SCOPUS.pdf http://irep.iium.edu.my/110947/ https://www.sciencedirect.com/science/article/pii/S2352340924000180?via%3Dihub
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spelling	my.iium.irep.1109472024-02-20T07:45:39Z http://irep.iium.edu.my/110947/ Baseline amplicon sequencing data for the ITS2 region in the green honey of Banggi Island, Sabah Ullah, Saeed Huda, Nurul Ab Wahab, Roswanira Abdul Hamid, Azzmer Azzar Mohd Nasir, Mohd Hamzah Mohamad, Mohd Azrul Naim Ahmad, Hajar Fauzan Oyewusi, Habeebat Adekilekun Huyop, Fahrul Q Science (General) Green honey, was discovered on Banggi Island, Sabah, showing high in essential amino acids and chlorophyll derivatives. Despite its lucrative market potential owing to its distinctive color, uncertainties persist regarding its nature. This study leverages amplicon sequencing by targeting micro- and macro-organisms present in honey environmental DNA (eDNA) using Internal Transcribed Spacer 2 (ITS2) region, enabling the identification of floral and microorganism sources that represent the honey's composition. The investigation into green honey from Banggi Island concerns the prevalence of honey adulteration and authenticity for economic gain. Adulteration methods, such as the addition of sugar syrups, compromise honey purity. Using a sequencing approach would help in determining the geographic origin and verifying the authenticity of the honey. The study aims to identify plant species or microorganisms in honey's eDNA. To authenticate honey, we utilized ITS2 with Illumina sequencing, exploring the diversity of green honey samples. Raw sequence reads obtained for the green honey sample revealed 1,438,627 raw reads, with a GC average of 49.22 %. A total of 44 amplicon sequence variances (ASVs) were identified, including three genera: Zygosaccharomyces with two species, Fraxinus with three species, and the genus Ficaria with only one species. Their respective relative abundances were 98.55%, 0.94%, and 0.51%. Zygosaccharomyces rouxii and Zygosaccharomyces mellis were identified as the pre-dominant yeast species in honey, while the Fraxinus and Ficaria genus represent common plant species in Sabah, particularly in Banggi Island. The dominance of Zygosaccharomyces species aligns with their known prevalence in honey, affirming the reliability of our findings. The presence of Fraxinus and Ficaria in the honey sample correlates with its abundance in the local environment. This amplicon sequencing approach not only contributes to our understanding of green honey composition but also serves as a valuable resource for authenticating honey origin in Malaysia, particularly for green honey from Banggi Island, Sabah. Our study pioneers the application of ITS2 amplicon sequencing for green honey amplicon sequencing, providing valuable insights into its composition and origin. This methodology, with a focus on eDNA, contributes to the authentication and quality determination of honey in Malaysia, addressing the pressing concerns of adulteration and variability in production practices. Elsevier 2024-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/110947/1/110947_Baseline%20amplicon%20sequencing%20data.pdf application/pdf en http://irep.iium.edu.my/110947/2/110947_Baseline%20amplicon%20sequencing%20data_SCOPUS.pdf Ullah, Saeed and Huda, Nurul and Ab Wahab, Roswanira and Abdul Hamid, Azzmer Azzar and Mohd Nasir, Mohd Hamzah and Mohamad, Mohd Azrul Naim and Ahmad, Hajar Fauzan and Oyewusi, Habeebat Adekilekun and Huyop, Fahrul (2024) Baseline amplicon sequencing data for the ITS2 region in the green honey of Banggi Island, Sabah. Data in Brief, 52 (1). pp. 1-8. E-ISSN 2352-3409 https://www.sciencedirect.com/science/article/pii/S2352340924000180?via%3Dihub 10.1016/j.dib.2024.110044
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Ullah, Saeed Huda, Nurul Ab Wahab, Roswanira Abdul Hamid, Azzmer Azzar Mohd Nasir, Mohd Hamzah Mohamad, Mohd Azrul Naim Ahmad, Hajar Fauzan Oyewusi, Habeebat Adekilekun Huyop, Fahrul Baseline amplicon sequencing data for the ITS2 region in the green honey of Banggi Island, Sabah
description	Green honey, was discovered on Banggi Island, Sabah, showing high in essential amino acids and chlorophyll derivatives. Despite its lucrative market potential owing to its distinctive color, uncertainties persist regarding its nature. This study leverages amplicon sequencing by targeting micro- and macro-organisms present in honey environmental DNA (eDNA) using Internal Transcribed Spacer 2 (ITS2) region, enabling the identification of floral and microorganism sources that represent the honey's composition. The investigation into green honey from Banggi Island concerns the prevalence of honey adulteration and authenticity for economic gain. Adulteration methods, such as the addition of sugar syrups, compromise honey purity. Using a sequencing approach would help in determining the geographic origin and verifying the authenticity of the honey. The study aims to identify plant species or microorganisms in honey's eDNA. To authenticate honey, we utilized ITS2 with Illumina sequencing, exploring the diversity of green honey samples. Raw sequence reads obtained for the green honey sample revealed 1,438,627 raw reads, with a GC average of 49.22 %. A total of 44 amplicon sequence variances (ASVs) were identified, including three genera: Zygosaccharomyces with two species, Fraxinus with three species, and the genus Ficaria with only one species. Their respective relative abundances were 98.55%, 0.94%, and 0.51%. Zygosaccharomyces rouxii and Zygosaccharomyces mellis were identified as the pre-dominant yeast species in honey, while the Fraxinus and Ficaria genus represent common plant species in Sabah, particularly in Banggi Island. The dominance of Zygosaccharomyces species aligns with their known prevalence in honey, affirming the reliability of our findings. The presence of Fraxinus and Ficaria in the honey sample correlates with its abundance in the local environment. This amplicon sequencing approach not only contributes to our understanding of green honey composition but also serves as a valuable resource for authenticating honey origin in Malaysia, particularly for green honey from Banggi Island, Sabah. Our study pioneers the application of ITS2 amplicon sequencing for green honey amplicon sequencing, providing valuable insights into its composition and origin. This methodology, with a focus on eDNA, contributes to the authentication and quality determination of honey in Malaysia, addressing the pressing concerns of adulteration and variability in production practices.
format	Article
author	Ullah, Saeed Huda, Nurul Ab Wahab, Roswanira Abdul Hamid, Azzmer Azzar Mohd Nasir, Mohd Hamzah Mohamad, Mohd Azrul Naim Ahmad, Hajar Fauzan Oyewusi, Habeebat Adekilekun Huyop, Fahrul
author_facet	Ullah, Saeed Huda, Nurul Ab Wahab, Roswanira Abdul Hamid, Azzmer Azzar Mohd Nasir, Mohd Hamzah Mohamad, Mohd Azrul Naim Ahmad, Hajar Fauzan Oyewusi, Habeebat Adekilekun Huyop, Fahrul
author_sort	Ullah, Saeed
title	Baseline amplicon sequencing data for the ITS2 region in the green honey of Banggi Island, Sabah
title_short	Baseline amplicon sequencing data for the ITS2 region in the green honey of Banggi Island, Sabah
title_full	Baseline amplicon sequencing data for the ITS2 region in the green honey of Banggi Island, Sabah
title_fullStr	Baseline amplicon sequencing data for the ITS2 region in the green honey of Banggi Island, Sabah
title_full_unstemmed	Baseline amplicon sequencing data for the ITS2 region in the green honey of Banggi Island, Sabah
title_sort	baseline amplicon sequencing data for the its2 region in the green honey of banggi island, sabah
publisher	Elsevier
publishDate	2024
url	http://irep.iium.edu.my/110947/1/110947_Baseline%20amplicon%20sequencing%20data.pdf http://irep.iium.edu.my/110947/2/110947_Baseline%20amplicon%20sequencing%20data_SCOPUS.pdf http://irep.iium.edu.my/110947/ https://www.sciencedirect.com/science/article/pii/S2352340924000180?via%3Dihub
_version_	1792146473678274560
score	13.211869

Baseline amplicon sequencing data for the ITS2 region in the green honey of Banggi Island, Sabah

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