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Silver nanoparticles biogenically synthesised using Maclurodendron porteri extract and their bioactivities

Silver nanoparticle is widely used in various field including medical, cosmetic, food and industrial purposes due to their unique properties in electrical conductivity, thermal, and biological activities. In the medical field, silver nanoparticles (AgNPs) have been reported to have strong antimicro...

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Main Authors: Badrillah, Nadhirah, Darnis, Deny Susanti, Tengku Mohd. Kamil, Tengku Karmila, Swandiny, Greesty Finotory, Widyastuti, Yuli, Zaini, Erizal, Bakhtiar, M. Taher
Format: Article
Language:English
English
Published: Elsevier Ltd. 2024
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/110866/3/110866_Silver%20nanoparticles%20biogenically%20synthesised.pdf
http://irep.iium.edu.my/110866/9/110866_Silver%20nanoparticles%20biogenically%20synthesised_SCOPUS.pdf
http://irep.iium.edu.my/110866/
https://www.sciencedirect.com/science/article/pii/S2405844024014853
https://doi.org/10.1016/j.heliyon.2024.e25454
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spelling my.iium.irep.1108662024-07-31T00:36:24Z http://irep.iium.edu.my/110866/ Silver nanoparticles biogenically synthesised using Maclurodendron porteri extract and their bioactivities Badrillah, Nadhirah Darnis, Deny Susanti Tengku Mohd. Kamil, Tengku Karmila Swandiny, Greesty Finotory Widyastuti, Yuli Zaini, Erizal Bakhtiar, M. Taher QD Chemistry Silver nanoparticle is widely used in various field including medical, cosmetic, food and industrial purposes due to their unique properties in electrical conductivity, thermal, and biological activities. In the medical field, silver nanoparticles (AgNPs) have been reported to have strong antimicrobial and cytotoxic activities. This study aimed to synthesize and characterize silver nanoparticles (AgNPs) using Maclurodendron porteri (MP) extract and to evaluate the antimicrobial and cytotoxic activities of the synthesised MP-AgNPs. Green method of Ultrasound Assisted Extraction (UAE) was used to extract the leaves of M. porter. Liquid Chromatography-Mass Spectrometry/Quadrupole time-of-flight (LC-MS/QTOF) was used to identify the compounds in the leaf extract of M. porteri. Characterisation of the synthesised nanoparticles involved ultraviolet–visible (UV–Vis), Fourier Transform Infrared (FTIR), scanning electromagnetic microscopy (SEM), Zeta potential Analyzer and Particle Size Analyzer. The cytotoxic assay was conducted on MCF-7 and Caco-2 cell lines by MTT assay. Antimicrobial activity was tested on Gram-negative and Gram-positive bacteria using the disc diffusion method. Based on LC-MS/QTOF analysis, 430 compounds were found. The identified major compounds consist of amino acids, polyphenols, steroids, terpenoids and heterocyclic compounds which possibly act as reducing agents. 1 mM, 5 mM and 10 mM of silver nitrate solution were mixed with the leaf extract to form silver nanoparticles. 1.2 mg/ml of MP-AgNPs were found to have antibacterial activity againstB. subtilis, S. aureus, E. coli, and P. aeruginosa with inhibitory zones of 8.0 ±0.36 mm, 8.5 ±0.45 mm, 7.5 ±0.36 mm, and 9.0 ±0.40 mm respectively. MP-AgNPs showed no cytotoxic activity against Caco-2 and MCF-7 cells. In conclusion, the presence of major amine compounds such as 10,11-dihydro-10,11-dihydroxyprotriptyline and harderoporphyrin in the extract facilitated the synthesis of AgNPs and the nanoparticle showed weak bioactivities in the assay conducted. Elsevier Ltd. 2024-02-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/110866/3/110866_Silver%20nanoparticles%20biogenically%20synthesised.pdf application/pdf en http://irep.iium.edu.my/110866/9/110866_Silver%20nanoparticles%20biogenically%20synthesised_SCOPUS.pdf Badrillah, Nadhirah and Darnis, Deny Susanti and Tengku Mohd. Kamil, Tengku Karmila and Swandiny, Greesty Finotory and Widyastuti, Yuli and Zaini, Erizal and Bakhtiar, M. Taher (2024) Silver nanoparticles biogenically synthesised using Maclurodendron porteri extract and their bioactivities. Heliyon, 10 (4). pp. 1-11. E-ISSN 2405-8440 https://www.sciencedirect.com/science/article/pii/S2405844024014853 https://doi.org/10.1016/j.heliyon.2024.e25454
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
spellingShingle QD Chemistry
Badrillah, Nadhirah
Darnis, Deny Susanti
Tengku Mohd. Kamil, Tengku Karmila
Swandiny, Greesty Finotory
Widyastuti, Yuli
Zaini, Erizal
Bakhtiar, M. Taher
Silver nanoparticles biogenically synthesised using Maclurodendron porteri extract and their bioactivities
description Silver nanoparticle is widely used in various field including medical, cosmetic, food and industrial purposes due to their unique properties in electrical conductivity, thermal, and biological activities. In the medical field, silver nanoparticles (AgNPs) have been reported to have strong antimicrobial and cytotoxic activities. This study aimed to synthesize and characterize silver nanoparticles (AgNPs) using Maclurodendron porteri (MP) extract and to evaluate the antimicrobial and cytotoxic activities of the synthesised MP-AgNPs. Green method of Ultrasound Assisted Extraction (UAE) was used to extract the leaves of M. porter. Liquid Chromatography-Mass Spectrometry/Quadrupole time-of-flight (LC-MS/QTOF) was used to identify the compounds in the leaf extract of M. porteri. Characterisation of the synthesised nanoparticles involved ultraviolet–visible (UV–Vis), Fourier Transform Infrared (FTIR), scanning electromagnetic microscopy (SEM), Zeta potential Analyzer and Particle Size Analyzer. The cytotoxic assay was conducted on MCF-7 and Caco-2 cell lines by MTT assay. Antimicrobial activity was tested on Gram-negative and Gram-positive bacteria using the disc diffusion method. Based on LC-MS/QTOF analysis, 430 compounds were found. The identified major compounds consist of amino acids, polyphenols, steroids, terpenoids and heterocyclic compounds which possibly act as reducing agents. 1 mM, 5 mM and 10 mM of silver nitrate solution were mixed with the leaf extract to form silver nanoparticles. 1.2 mg/ml of MP-AgNPs were found to have antibacterial activity againstB. subtilis, S. aureus, E. coli, and P. aeruginosa with inhibitory zones of 8.0 ±0.36 mm, 8.5 ±0.45 mm, 7.5 ±0.36 mm, and 9.0 ±0.40 mm respectively. MP-AgNPs showed no cytotoxic activity against Caco-2 and MCF-7 cells. In conclusion, the presence of major amine compounds such as 10,11-dihydro-10,11-dihydroxyprotriptyline and harderoporphyrin in the extract facilitated the synthesis of AgNPs and the nanoparticle showed weak bioactivities in the assay conducted.
format Article
author Badrillah, Nadhirah
Darnis, Deny Susanti
Tengku Mohd. Kamil, Tengku Karmila
Swandiny, Greesty Finotory
Widyastuti, Yuli
Zaini, Erizal
Bakhtiar, M. Taher
author_facet Badrillah, Nadhirah
Darnis, Deny Susanti
Tengku Mohd. Kamil, Tengku Karmila
Swandiny, Greesty Finotory
Widyastuti, Yuli
Zaini, Erizal
Bakhtiar, M. Taher
author_sort Badrillah, Nadhirah
title Silver nanoparticles biogenically synthesised using Maclurodendron porteri extract and their bioactivities
title_short Silver nanoparticles biogenically synthesised using Maclurodendron porteri extract and their bioactivities
title_full Silver nanoparticles biogenically synthesised using Maclurodendron porteri extract and their bioactivities
title_fullStr Silver nanoparticles biogenically synthesised using Maclurodendron porteri extract and their bioactivities
title_full_unstemmed Silver nanoparticles biogenically synthesised using Maclurodendron porteri extract and their bioactivities
title_sort silver nanoparticles biogenically synthesised using maclurodendron porteri extract and their bioactivities
publisher Elsevier Ltd.
publishDate 2024
url http://irep.iium.edu.my/110866/3/110866_Silver%20nanoparticles%20biogenically%20synthesised.pdf
http://irep.iium.edu.my/110866/9/110866_Silver%20nanoparticles%20biogenically%20synthesised_SCOPUS.pdf
http://irep.iium.edu.my/110866/
https://www.sciencedirect.com/science/article/pii/S2405844024014853
https://doi.org/10.1016/j.heliyon.2024.e25454
_version_ 1806442131858915328
score 13.211869

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