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High performance liquid chromatographic method optimized by Box-Behnken design model to determine caffeine in pharmaceutical preparations and urine samples

A UV-HPLC method optimized by Box-Behnken design model was developed to determine caffeine in pharmaceutical preparations and urine samples. The chromatographic conditions followed were mobile phase: methanol/water/ citrate buffer (pH 4.6) (40:25:35, v/v/v), AcclaimTM Dionex C18 column (ODS 100A˚, 5...

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Main Authors: Azmi, Syed Najmul Hejaz, Nasir Al Rawahi, Wafa Aqib, Ibrahim Al Yahyai, Arwa, Ali Al Qasimi, Asma, Saif Al Fuliti, Kothar, Said Al Qalhati, Omayma, Rahman, Nafisur, Ahmed, Qamar Uddin
Format: Article
Language:English
English
Published: Elsevier 2024
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/110717/1/110717_High%20performance%20liquid%20chromatographic%20method%20optimized%20by%20Box-Behnken%20design%20model.pdf
http://irep.iium.edu.my/110717/7/110717_High%20performance%20liquid%20chromatographic%20method%20optimized%20by%20Box-Behnken%20design%20model_SCOPUS.pdf
http://irep.iium.edu.my/110717/
https://www.sciencedirect.com/science/article/abs/pii/S1570023224000436
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spelling my.iium.irep.1107172024-05-21T03:37:47Z http://irep.iium.edu.my/110717/ High performance liquid chromatographic method optimized by Box-Behnken design model to determine caffeine in pharmaceutical preparations and urine samples Azmi, Syed Najmul Hejaz Nasir Al Rawahi, Wafa Aqib Ibrahim Al Yahyai, Arwa Ali Al Qasimi, Asma Saif Al Fuliti, Kothar Said Al Qalhati, Omayma Rahman, Nafisur Ahmed, Qamar Uddin QD Chemistry A UV-HPLC method optimized by Box-Behnken design model was developed to determine caffeine in pharmaceutical preparations and urine samples. The chromatographic conditions followed were mobile phase: methanol/water/ citrate buffer (pH 4.6) (40:25:35, v/v/v), AcclaimTM Dionex C18 column (ODS 100A˚, 5 µm; 4.6 × 250 mm), flow rate (0.9 mL min− 1), column temperature (30 ◦C) and UV-detection wavelength (204 nm). The chromatographic variables: pH (A), % methanol fraction (B), flow rate (C) and column temperature (D) were optimized at 50 μg mL− 1 caffeine using BBD model. The chromatogram resulted in the asymmetry factor (1.23), theoretical plate 13,786 and retention time (5.79 min). The proposed HPLC method’s greenness point was assessed by Analytical Eco-scale and found to be 78 (as per guidelines, ranked as excellent). The linearity was ranged from 2.0 to 70 µg mL− 1 with coefficient of correlation (r = 0.999) and detection limit of 0.19 µg mL− 1. The proposed method was developed successfully and applied for the assay of active caffeine in pharmaceutical preparations and urine samples. The % recovery obtained by both (proposed and reference) methods ranged from 99.98 to 100.05 % followed the compliance (100 ± 2 %) with Canadian Health Protection regulatory guidelines. The performance of the proposed method was compared with published papers and found to be acceptable and superior. The proposed method was quite effective as the reference method, and hence can be used as an alternative method for the assay of active caffeine in pharmaceutical preparations and urine samples. Elsevier 2024-02-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/110717/1/110717_High%20performance%20liquid%20chromatographic%20method%20optimized%20by%20Box-Behnken%20design%20model.pdf application/pdf en http://irep.iium.edu.my/110717/7/110717_High%20performance%20liquid%20chromatographic%20method%20optimized%20by%20Box-Behnken%20design%20model_SCOPUS.pdf Azmi, Syed Najmul Hejaz and Nasir Al Rawahi, Wafa Aqib and Ibrahim Al Yahyai, Arwa and Ali Al Qasimi, Asma and Saif Al Fuliti, Kothar and Said Al Qalhati, Omayma and Rahman, Nafisur and Ahmed, Qamar Uddin (2024) High performance liquid chromatographic method optimized by Box-Behnken design model to determine caffeine in pharmaceutical preparations and urine samples. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1234. ISSN 1570-0232 E-ISSN 1873-376X https://www.sciencedirect.com/science/article/abs/pii/S1570023224000436 10.1016/j.jchromb.2024.124035
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
spellingShingle QD Chemistry
Azmi, Syed Najmul Hejaz
Nasir Al Rawahi, Wafa Aqib
Ibrahim Al Yahyai, Arwa
Ali Al Qasimi, Asma
Saif Al Fuliti, Kothar
Said Al Qalhati, Omayma
Rahman, Nafisur
Ahmed, Qamar Uddin
High performance liquid chromatographic method optimized by Box-Behnken design model to determine caffeine in pharmaceutical preparations and urine samples
description A UV-HPLC method optimized by Box-Behnken design model was developed to determine caffeine in pharmaceutical preparations and urine samples. The chromatographic conditions followed were mobile phase: methanol/water/ citrate buffer (pH 4.6) (40:25:35, v/v/v), AcclaimTM Dionex C18 column (ODS 100A˚, 5 µm; 4.6 × 250 mm), flow rate (0.9 mL min− 1), column temperature (30 ◦C) and UV-detection wavelength (204 nm). The chromatographic variables: pH (A), % methanol fraction (B), flow rate (C) and column temperature (D) were optimized at 50 μg mL− 1 caffeine using BBD model. The chromatogram resulted in the asymmetry factor (1.23), theoretical plate 13,786 and retention time (5.79 min). The proposed HPLC method’s greenness point was assessed by Analytical Eco-scale and found to be 78 (as per guidelines, ranked as excellent). The linearity was ranged from 2.0 to 70 µg mL− 1 with coefficient of correlation (r = 0.999) and detection limit of 0.19 µg mL− 1. The proposed method was developed successfully and applied for the assay of active caffeine in pharmaceutical preparations and urine samples. The % recovery obtained by both (proposed and reference) methods ranged from 99.98 to 100.05 % followed the compliance (100 ± 2 %) with Canadian Health Protection regulatory guidelines. The performance of the proposed method was compared with published papers and found to be acceptable and superior. The proposed method was quite effective as the reference method, and hence can be used as an alternative method for the assay of active caffeine in pharmaceutical preparations and urine samples.
format Article
author Azmi, Syed Najmul Hejaz
Nasir Al Rawahi, Wafa Aqib
Ibrahim Al Yahyai, Arwa
Ali Al Qasimi, Asma
Saif Al Fuliti, Kothar
Said Al Qalhati, Omayma
Rahman, Nafisur
Ahmed, Qamar Uddin
author_facet Azmi, Syed Najmul Hejaz
Nasir Al Rawahi, Wafa Aqib
Ibrahim Al Yahyai, Arwa
Ali Al Qasimi, Asma
Saif Al Fuliti, Kothar
Said Al Qalhati, Omayma
Rahman, Nafisur
Ahmed, Qamar Uddin
author_sort Azmi, Syed Najmul Hejaz
title High performance liquid chromatographic method optimized by Box-Behnken design model to determine caffeine in pharmaceutical preparations and urine samples
title_short High performance liquid chromatographic method optimized by Box-Behnken design model to determine caffeine in pharmaceutical preparations and urine samples
title_full High performance liquid chromatographic method optimized by Box-Behnken design model to determine caffeine in pharmaceutical preparations and urine samples
title_fullStr High performance liquid chromatographic method optimized by Box-Behnken design model to determine caffeine in pharmaceutical preparations and urine samples
title_full_unstemmed High performance liquid chromatographic method optimized by Box-Behnken design model to determine caffeine in pharmaceutical preparations and urine samples
title_sort high performance liquid chromatographic method optimized by box-behnken design model to determine caffeine in pharmaceutical preparations and urine samples
publisher Elsevier
publishDate 2024
url http://irep.iium.edu.my/110717/1/110717_High%20performance%20liquid%20chromatographic%20method%20optimized%20by%20Box-Behnken%20design%20model.pdf
http://irep.iium.edu.my/110717/7/110717_High%20performance%20liquid%20chromatographic%20method%20optimized%20by%20Box-Behnken%20design%20model_SCOPUS.pdf
http://irep.iium.edu.my/110717/
https://www.sciencedirect.com/science/article/abs/pii/S1570023224000436
_version_ 1800081781173518336
score 13.211869

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