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Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos

Gelidium spinosum is an edible red seaweed from the family Gelidiaceae with possibility to be developed. The potential medical benefits of G. spinosum have been established, yet adequate empirical research on its toxicity is still lacking. Hence, the present work was aimed to examine the toxicity of...

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Main Authors: Warsi, Warsi, Jaswir, Irwandi, Ahmed, Qamar Uddin, Mahfudh, Nurkhasanah, Mohd. Nawi, Mohamed Sufian, Rohman, Abdul, Khatib, Alfi
Format: Article
Language:English
English
Published: Pensoft Publishers 2024
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/110716/2/110716_Morphological%2C%20teratogenic%20and%20behavioral%20evaluations%20of%20Gelidium%20spinosum.pdf
http://irep.iium.edu.my/110716/8/110716_Morphological%2C%20teratogenic%20and%20behavioral%20evaluations%20of%20Gelidium%20spinosum_SCOPUS.pdf
http://irep.iium.edu.my/110716/
https://pharmacia.pensoft.net/article/109918/
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spelling my.iium.irep.1107162024-07-29T09:36:55Z http://irep.iium.edu.my/110716/ Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos Warsi, Warsi Jaswir, Irwandi Ahmed, Qamar Uddin Mahfudh, Nurkhasanah Mohd. Nawi, Mohamed Sufian Rohman, Abdul Khatib, Alfi QD Chemistry Gelidium spinosum is an edible red seaweed from the family Gelidiaceae with possibility to be developed. The potential medical benefits of G. spinosum have been established, yet adequate empirical research on its toxicity is still lacking. Hence, the present work was aimed to examine the toxicity of G. spinosum methanol extract (GsME) on zebrafish (Danio rerio) embryos and to identify the phytoconstituents using gas chromatography-mass spectrometry (GC-MS) analysis. Results of this study showed that GsME induced morphological defects in zebrafish embryos, including reduction in eye size and body length. Moreover, yolk sac size and mortality were increased in zebrafish embryos exposed to GsME in a dose-dependent manner. GsME at high concentrations triggered teratogenic effects in zebrafish embryos such as decrease in heartbeat/minute, lack of pigmentation, lack of somite, structural deformity, pericardial oedema, and yolk oedema. The LC50 and EC50 of GsME were < 100 mg/L, which classified as a harmful category. The teratogenic index of GsME was found to be > 1, indicating its teratogenic attribute. Additionally, GsME exerted behavioral effects i.e. significantly lower total distance of movement and slower swimming speed of zebrafish embryos. GC-MS analysis of GsME was confirmed the presence of amino acid, phenolics, carboxylic acids, reducing sugars, saturated fatty acids and brominated saturated fatty acid in the extract. It suggesting that compounds of 13-bromotetradecanoic acid, palmitic acid, and stearic acid containing in GsME were contributed to the toxic effects. Pensoft Publishers 2024-01-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/110716/2/110716_Morphological%2C%20teratogenic%20and%20behavioral%20evaluations%20of%20Gelidium%20spinosum.pdf application/pdf en http://irep.iium.edu.my/110716/8/110716_Morphological%2C%20teratogenic%20and%20behavioral%20evaluations%20of%20Gelidium%20spinosum_SCOPUS.pdf Warsi, Warsi and Jaswir, Irwandi and Ahmed, Qamar Uddin and Mahfudh, Nurkhasanah and Mohd. Nawi, Mohamed Sufian and Rohman, Abdul and Khatib, Alfi (2024) Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos. Pharmacia, 71. pp. 1-10. ISSN 0428-0296 E-ISSN 2603-557X https://pharmacia.pensoft.net/article/109918/ 10.3897/pharmacia.71.e109918
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
spellingShingle QD Chemistry
Warsi, Warsi
Jaswir, Irwandi
Ahmed, Qamar Uddin
Mahfudh, Nurkhasanah
Mohd. Nawi, Mohamed Sufian
Rohman, Abdul
Khatib, Alfi
Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos
description Gelidium spinosum is an edible red seaweed from the family Gelidiaceae with possibility to be developed. The potential medical benefits of G. spinosum have been established, yet adequate empirical research on its toxicity is still lacking. Hence, the present work was aimed to examine the toxicity of G. spinosum methanol extract (GsME) on zebrafish (Danio rerio) embryos and to identify the phytoconstituents using gas chromatography-mass spectrometry (GC-MS) analysis. Results of this study showed that GsME induced morphological defects in zebrafish embryos, including reduction in eye size and body length. Moreover, yolk sac size and mortality were increased in zebrafish embryos exposed to GsME in a dose-dependent manner. GsME at high concentrations triggered teratogenic effects in zebrafish embryos such as decrease in heartbeat/minute, lack of pigmentation, lack of somite, structural deformity, pericardial oedema, and yolk oedema. The LC50 and EC50 of GsME were < 100 mg/L, which classified as a harmful category. The teratogenic index of GsME was found to be > 1, indicating its teratogenic attribute. Additionally, GsME exerted behavioral effects i.e. significantly lower total distance of movement and slower swimming speed of zebrafish embryos. GC-MS analysis of GsME was confirmed the presence of amino acid, phenolics, carboxylic acids, reducing sugars, saturated fatty acids and brominated saturated fatty acid in the extract. It suggesting that compounds of 13-bromotetradecanoic acid, palmitic acid, and stearic acid containing in GsME were contributed to the toxic effects.
format Article
author Warsi, Warsi
Jaswir, Irwandi
Ahmed, Qamar Uddin
Mahfudh, Nurkhasanah
Mohd. Nawi, Mohamed Sufian
Rohman, Abdul
Khatib, Alfi
author_facet Warsi, Warsi
Jaswir, Irwandi
Ahmed, Qamar Uddin
Mahfudh, Nurkhasanah
Mohd. Nawi, Mohamed Sufian
Rohman, Abdul
Khatib, Alfi
author_sort Warsi, Warsi
title Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos
title_short Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos
title_full Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos
title_fullStr Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos
title_full_unstemmed Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos
title_sort morphological, teratogenic and behavioral evaluations of gelidium spinosum methanol extract on zebrafish embryos
publisher Pensoft Publishers
publishDate 2024
url http://irep.iium.edu.my/110716/2/110716_Morphological%2C%20teratogenic%20and%20behavioral%20evaluations%20of%20Gelidium%20spinosum.pdf
http://irep.iium.edu.my/110716/8/110716_Morphological%2C%20teratogenic%20and%20behavioral%20evaluations%20of%20Gelidium%20spinosum_SCOPUS.pdf
http://irep.iium.edu.my/110716/
https://pharmacia.pensoft.net/article/109918/
_version_ 1806442130926731264
score 13.211869

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