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The photobiomodulation effect on L6 cells viability and migration by different exposure duration of 532 nm low-level laser

Introduction: Low-level laser has been widely researched and practiced in various medical fields due to its photobiomodulation effects towards diverse types of cells. The effectiveness of photobiomodulation depends on factors such as wavelength, power, and duration of exposure. In this work, we exam...

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Main Authors: Hud, Idris, Suardi, Nursakinah, Mohd Nasir, Mohd Hamzah
Format: Article
Language:English
Published: Faculty of Medicine, UKM 2023
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/110273/1/110273_The%20photobiomodulation%20effect.pdf
http://irep.iium.edu.my/110273/
https://www.medicineandhealthukm.com/article/international-virtual-medical-research-symposium
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spelling my.iium.irep.1102732024-01-19T07:24:03Z http://irep.iium.edu.my/110273/ The photobiomodulation effect on L6 cells viability and migration by different exposure duration of 532 nm low-level laser Hud, Idris Suardi, Nursakinah Mohd Nasir, Mohd Hamzah Q Science (General) Introduction: Low-level laser has been widely researched and practiced in various medical fields due to its photobiomodulation effects towards diverse types of cells. The effectiveness of photobiomodulation depends on factors such as wavelength, power, and duration of exposure. In this work, we examined the effects of varying exposure durations of 0.1W 532 nm low-level laser light on the viability and migration of L6 rat myoblast cells (CRL-1458) under both direct and indirect irradiation conditions. Exposure times ranged from 30 to 300 seconds. Materials and Methods: For direct exposure, L6 cells were directly exposed to 532 nm laser, whereas for indirect exposure the media was first exposed then the cells were added. After laser irradiation, cells are incubated for another 24-hours before proceeding to conduct the assays on cell viability and migration. The viability of cells was measured through MTT assay. In exploring the effect of laser towards the cell migration, scratch assay was done. Real-time imaging was captured and analyzed in ImageJ software. Results: Results revealed that direct irradiation significantly improved the cell viability (p<0.05) and slightly induced the cell migration compared to indirect irradiation. When irradiated directly for 120s, the rate of migration of the L6 cells increased 14.07% and the area covered increased 16.13% compared to control. When the cells were irradiated directly above 300s, the cells demonstrated significant inhibition (p<0.05). Conclusion: This work using direct low-level laser irradiation on cells demonstrated a significant effect on cell viability and migration hence it has potential to be utilized as a promising noninvasive approach in muscle repair treatment. This study serves as a foundation on the optimal exposure duration of 532 nm low-level laser for therapeutic use targeted to muscle cells. Faculty of Medicine, UKM 2023-12 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/110273/1/110273_The%20photobiomodulation%20effect.pdf Hud, Idris and Suardi, Nursakinah and Mohd Nasir, Mohd Hamzah (2023) The photobiomodulation effect on L6 cells viability and migration by different exposure duration of 532 nm low-level laser. Medicine and Health, 18 (Suppl. 7). p. 138. ISSN 1823-2140 E-ISSN 2289-5728 https://www.medicineandhealthukm.com/article/international-virtual-medical-research-symposium 10.17576/MH.2023.s1807
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Hud, Idris
Suardi, Nursakinah
Mohd Nasir, Mohd Hamzah
The photobiomodulation effect on L6 cells viability and migration by different exposure duration of 532 nm low-level laser
description Introduction: Low-level laser has been widely researched and practiced in various medical fields due to its photobiomodulation effects towards diverse types of cells. The effectiveness of photobiomodulation depends on factors such as wavelength, power, and duration of exposure. In this work, we examined the effects of varying exposure durations of 0.1W 532 nm low-level laser light on the viability and migration of L6 rat myoblast cells (CRL-1458) under both direct and indirect irradiation conditions. Exposure times ranged from 30 to 300 seconds. Materials and Methods: For direct exposure, L6 cells were directly exposed to 532 nm laser, whereas for indirect exposure the media was first exposed then the cells were added. After laser irradiation, cells are incubated for another 24-hours before proceeding to conduct the assays on cell viability and migration. The viability of cells was measured through MTT assay. In exploring the effect of laser towards the cell migration, scratch assay was done. Real-time imaging was captured and analyzed in ImageJ software. Results: Results revealed that direct irradiation significantly improved the cell viability (p<0.05) and slightly induced the cell migration compared to indirect irradiation. When irradiated directly for 120s, the rate of migration of the L6 cells increased 14.07% and the area covered increased 16.13% compared to control. When the cells were irradiated directly above 300s, the cells demonstrated significant inhibition (p<0.05). Conclusion: This work using direct low-level laser irradiation on cells demonstrated a significant effect on cell viability and migration hence it has potential to be utilized as a promising noninvasive approach in muscle repair treatment. This study serves as a foundation on the optimal exposure duration of 532 nm low-level laser for therapeutic use targeted to muscle cells.
format Article
author Hud, Idris
Suardi, Nursakinah
Mohd Nasir, Mohd Hamzah
author_facet Hud, Idris
Suardi, Nursakinah
Mohd Nasir, Mohd Hamzah
author_sort Hud, Idris
title The photobiomodulation effect on L6 cells viability and migration by different exposure duration of 532 nm low-level laser
title_short The photobiomodulation effect on L6 cells viability and migration by different exposure duration of 532 nm low-level laser
title_full The photobiomodulation effect on L6 cells viability and migration by different exposure duration of 532 nm low-level laser
title_fullStr The photobiomodulation effect on L6 cells viability and migration by different exposure duration of 532 nm low-level laser
title_full_unstemmed The photobiomodulation effect on L6 cells viability and migration by different exposure duration of 532 nm low-level laser
title_sort photobiomodulation effect on l6 cells viability and migration by different exposure duration of 532 nm low-level laser
publisher Faculty of Medicine, UKM
publishDate 2023
url http://irep.iium.edu.my/110273/1/110273_The%20photobiomodulation%20effect.pdf
http://irep.iium.edu.my/110273/
https://www.medicineandhealthukm.com/article/international-virtual-medical-research-symposium
_version_ 1789424069386436608
score 13.211869

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