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The cytotoxic effect of Cananga odorata (CO) essential oil on skin cancer is not via TP53 gene expression modulation

Introduction: Skin cancer is one of the most common cancers worldwide. Cananga odorata (CO) essential oil (EO) has been reported to have anticancer and antioxidant properties. Hence, it may have the potential to be used as a topical treatment for skin cancer. The aim of this study is to assess the c...

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Main Author: Anuar, Muhammad Ashraf Fithri
Format: Article
Language:English
English
Published: 2023
Subjects:
QP Physiology
R Medicine (General)
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
RV Botanic, Thomsonian, and eclectic medicine
Online Access:http://irep.iium.edu.my/109648/18/109648_The%20cytotoxic%20effect%20of%20Canaga%20odorata%20%28CO%29.pdf
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spelling my.iium.irep.1096482024-01-24T15:33:29Z http://irep.iium.edu.my/109648/ The cytotoxic effect of Cananga odorata (CO) essential oil on skin cancer is not via TP53 gene expression modulation Anuar, Muhammad Ashraf Fithri QP Physiology R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine Introduction: Skin cancer is one of the most common cancers worldwide. Cananga odorata (CO) essential oil (EO) has been reported to have anticancer and antioxidant properties. Hence, it may have the potential to be used as a topical treatment for skin cancer. The aim of this study is to assess the cytotoxic effect of CO EO on skin cancer, the pro-apoptotic TP53 gene expression, and its protein (p53) secretion. Materials and Methods: A431 squamous skin cancer cells and HFF1 fibroblast cells were treated with CO EO at 125, 250, and 500 μg/ml for 24 h. Their morphology was observed at 20-40x magnification. TP53 gene expression in these cells was examined by real-time PCR. p53 protein secretion in the conditioned media was assessed using western blot. Results: CO EO treated A431 cells exhibited loss of cellular margin and higher non-viable cells at all concentrations. Meanwhile, HFF1 cells treated with 125 μg/ml CO EO showed no significant changes. At 250 and 500 μg/ml, there was no significant changes in TP53 gene expression in A431 cells, but higher p53 protein secretion was observed. Conclusion: Our findings suggest that CO EO was cytotoxic to skin cancer at 250 and 500 μg/ml without augmenting TP53 gene expression. Hence, further investigations into CO EO effect on other apoptotic or anti-proliferative regulatory genes are required to establish its underlying molecular mechanism leading to its cytotoxic effect in skin cancer. 2023 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/109648/18/109648_The%20cytotoxic%20effect%20of%20Canaga%20odorata%20%28CO%29.pdf application/pdf en http://irep.iium.edu.my/109648/19/109648_The%20cytotoxic%20effect%20of%20Canaga%20odorata%20%28CO%29_Certificate.pdf Anuar, Muhammad Ashraf Fithri (2023) The cytotoxic effect of Cananga odorata (CO) essential oil on skin cancer is not via TP53 gene expression modulation. Medicine & Health.
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QP Physiology
R Medicine (General)
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
RV Botanic, Thomsonian, and eclectic medicine
spellingShingle QP Physiology
R Medicine (General)
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
RV Botanic, Thomsonian, and eclectic medicine
Anuar, Muhammad Ashraf Fithri
The cytotoxic effect of Cananga odorata (CO) essential oil on skin cancer is not via TP53 gene expression modulation
description Introduction: Skin cancer is one of the most common cancers worldwide. Cananga odorata (CO) essential oil (EO) has been reported to have anticancer and antioxidant properties. Hence, it may have the potential to be used as a topical treatment for skin cancer. The aim of this study is to assess the cytotoxic effect of CO EO on skin cancer, the pro-apoptotic TP53 gene expression, and its protein (p53) secretion. Materials and Methods: A431 squamous skin cancer cells and HFF1 fibroblast cells were treated with CO EO at 125, 250, and 500 μg/ml for 24 h. Their morphology was observed at 20-40x magnification. TP53 gene expression in these cells was examined by real-time PCR. p53 protein secretion in the conditioned media was assessed using western blot. Results: CO EO treated A431 cells exhibited loss of cellular margin and higher non-viable cells at all concentrations. Meanwhile, HFF1 cells treated with 125 μg/ml CO EO showed no significant changes. At 250 and 500 μg/ml, there was no significant changes in TP53 gene expression in A431 cells, but higher p53 protein secretion was observed. Conclusion: Our findings suggest that CO EO was cytotoxic to skin cancer at 250 and 500 μg/ml without augmenting TP53 gene expression. Hence, further investigations into CO EO effect on other apoptotic or anti-proliferative regulatory genes are required to establish its underlying molecular mechanism leading to its cytotoxic effect in skin cancer.
format Article
author Anuar, Muhammad Ashraf Fithri
author_facet Anuar, Muhammad Ashraf Fithri
author_sort Anuar, Muhammad Ashraf Fithri
title The cytotoxic effect of Cananga odorata (CO) essential oil on skin cancer is not via TP53 gene expression modulation
title_short The cytotoxic effect of Cananga odorata (CO) essential oil on skin cancer is not via TP53 gene expression modulation
title_full The cytotoxic effect of Cananga odorata (CO) essential oil on skin cancer is not via TP53 gene expression modulation
title_fullStr The cytotoxic effect of Cananga odorata (CO) essential oil on skin cancer is not via TP53 gene expression modulation
title_full_unstemmed The cytotoxic effect of Cananga odorata (CO) essential oil on skin cancer is not via TP53 gene expression modulation
title_sort cytotoxic effect of cananga odorata (co) essential oil on skin cancer is not via tp53 gene expression modulation
publishDate 2023
url http://irep.iium.edu.my/109648/18/109648_The%20cytotoxic%20effect%20of%20Canaga%20odorata%20%28CO%29.pdf
http://irep.iium.edu.my/109648/19/109648_The%20cytotoxic%20effect%20of%20Canaga%20odorata%20%28CO%29_Certificate.pdf
http://irep.iium.edu.my/109648/
_version_ 1789424034949103616
score 13.211869

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