Staff View: Bioactive strawberry fruit (Arbutus unedo L.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats

Bioactive strawberry fruit (Arbutus unedo L.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats

Background: Paraquat (1,1′-dimethyl-4-4′-bipyridinium dichloride) exposure is well-established as a neurotoxic agent capable of causing neurological deficits in offspring. This study aimed to investigate therapeutic effects of Arbutus unedo L. aqueous extract (AU) against paraquat (PQ) exposure....

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Main Authors:	Lhaj, Zakaria Ait, Ibork, Hind, El Idrissi, Sara, Lhaj, Farida Ait, Sobeh, Mansour, Mohamed, Wael Mohamed Yousef, Alamy, Meryem, Taghzouti, Khalid, Abboussi, Oualid
Format:	Article
Language:	English English
Published:	Frontiers Media S.A. 2023
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/107435/2/107435_Bioactive%20strawberry%20fruit%20%28Arbutus%20unedo%20L.%29.pdf http://irep.iium.edu.my/107435/8/107435_Bioactive%20strawberry%20fruit%20%28Arbutus%20unedo%20L.%29_Scopus.pdf http://irep.iium.edu.my/107435/ https://www.frontiersin.org/articles/10.3389/fnins.2023.1244603/full https://doi.org/10.3389/fnins.2023.1244603
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spelling	my.iium.irep.1074352023-12-05T07:38:39Z http://irep.iium.edu.my/107435/ Bioactive strawberry fruit (Arbutus unedo L.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats Lhaj, Zakaria Ait Ibork, Hind El Idrissi, Sara Lhaj, Farida Ait Sobeh, Mansour Mohamed, Wael Mohamed Yousef Alamy, Meryem Taghzouti, Khalid Abboussi, Oualid R Medicine (General) Background: Paraquat (1,1′-dimethyl-4-4′-bipyridinium dichloride) exposure is well-established as a neurotoxic agent capable of causing neurological deficits in offspring. This study aimed to investigate therapeutic effects of Arbutus unedo L. aqueous extract (AU) against paraquat (PQ) exposure. Methods: For that the phytoconstituents of AU was determined by LC/MS, and then its antioxidant potential was assessed by DPPH and ABTS assays. The assessment included its impact on cell viability and mitochondrial metabolism using N27 dopaminergic cells. Additionally, we evaluated the effects of prenatal PQ exposure on motor coordination, dopamine levels, trace element levels, and total antioxidant capacity (TAC) in rat progeny. Results: The phytochemical profile of AU extract revealed the presence of 35 compounds, primarily phenolic and organic acids, and flavonoids. This accounted for its strong in vitro antioxidant activities against DPPH and ABTS radicals, surpassing the activities of vitamin C. Our findings demonstrated that AU effectively inhibited PQ-induced loss of N27 rat dopaminergic neural cells and significantly enhanced their mitochondrial respiration. Furthermore, daily post-treatment with AU during the 21 days of the rat's pregnancy alleviated PQ-induced motor deficits and akinesia in rat progeny. These effects inhibited dopamine depletion and reduced iron levels in the striatal tissues. The observed outcomes appeared to be mediated by the robust antioxidant activity of AU, effectively counteracting the PQ-induced decrease in TAC in the blood plasma of rat progeny. These effects could be attributed to the bioactive compounds present in AU, including phenolic acids such as gallic acid and flavonoids such as quercetin, rutin, apigenin, glucuronide, and kaempferol, all known for their potent antioxidant capacity. Discussion: In conclusion, this preclinical study provided the first evidence of the therapeutic potential of AU extract against PQ-induced neurotoxicity. These findings emphasize the need for further exploration of the clinical applicability of AU in mitigating neurotoxin-induced brain damage. Frontiers Media S.A. 2023-10-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/107435/2/107435_Bioactive%20strawberry%20fruit%20%28Arbutus%20unedo%20L.%29.pdf application/pdf en http://irep.iium.edu.my/107435/8/107435_Bioactive%20strawberry%20fruit%20%28Arbutus%20unedo%20L.%29_Scopus.pdf Lhaj, Zakaria Ait and Ibork, Hind and El Idrissi, Sara and Lhaj, Farida Ait and Sobeh, Mansour and Mohamed, Wael Mohamed Yousef and Alamy, Meryem and Taghzouti, Khalid and Abboussi, Oualid (2023) Bioactive strawberry fruit (Arbutus unedo L.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats. Frontiers in Neuroscience, 17. pp. 1-15. ISSN 1662-4548 E-ISSN 1662-453X https://www.frontiersin.org/articles/10.3389/fnins.2023.1244603/full https://doi.org/10.3389/fnins.2023.1244603
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Lhaj, Zakaria Ait Ibork, Hind El Idrissi, Sara Lhaj, Farida Ait Sobeh, Mansour Mohamed, Wael Mohamed Yousef Alamy, Meryem Taghzouti, Khalid Abboussi, Oualid Bioactive strawberry fruit (Arbutus unedo L.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats
description	Background: Paraquat (1,1′-dimethyl-4-4′-bipyridinium dichloride) exposure is well-established as a neurotoxic agent capable of causing neurological deficits in offspring. This study aimed to investigate therapeutic effects of Arbutus unedo L. aqueous extract (AU) against paraquat (PQ) exposure. Methods: For that the phytoconstituents of AU was determined by LC/MS, and then its antioxidant potential was assessed by DPPH and ABTS assays. The assessment included its impact on cell viability and mitochondrial metabolism using N27 dopaminergic cells. Additionally, we evaluated the effects of prenatal PQ exposure on motor coordination, dopamine levels, trace element levels, and total antioxidant capacity (TAC) in rat progeny. Results: The phytochemical profile of AU extract revealed the presence of 35 compounds, primarily phenolic and organic acids, and flavonoids. This accounted for its strong in vitro antioxidant activities against DPPH and ABTS radicals, surpassing the activities of vitamin C. Our findings demonstrated that AU effectively inhibited PQ-induced loss of N27 rat dopaminergic neural cells and significantly enhanced their mitochondrial respiration. Furthermore, daily post-treatment with AU during the 21 days of the rat's pregnancy alleviated PQ-induced motor deficits and akinesia in rat progeny. These effects inhibited dopamine depletion and reduced iron levels in the striatal tissues. The observed outcomes appeared to be mediated by the robust antioxidant activity of AU, effectively counteracting the PQ-induced decrease in TAC in the blood plasma of rat progeny. These effects could be attributed to the bioactive compounds present in AU, including phenolic acids such as gallic acid and flavonoids such as quercetin, rutin, apigenin, glucuronide, and kaempferol, all known for their potent antioxidant capacity. Discussion: In conclusion, this preclinical study provided the first evidence of the therapeutic potential of AU extract against PQ-induced neurotoxicity. These findings emphasize the need for further exploration of the clinical applicability of AU in mitigating neurotoxin-induced brain damage.
format	Article
author	Lhaj, Zakaria Ait Ibork, Hind El Idrissi, Sara Lhaj, Farida Ait Sobeh, Mansour Mohamed, Wael Mohamed Yousef Alamy, Meryem Taghzouti, Khalid Abboussi, Oualid
author_facet	Lhaj, Zakaria Ait Ibork, Hind El Idrissi, Sara Lhaj, Farida Ait Sobeh, Mansour Mohamed, Wael Mohamed Yousef Alamy, Meryem Taghzouti, Khalid Abboussi, Oualid
author_sort	Lhaj, Zakaria Ait
title	Bioactive strawberry fruit (Arbutus unedo L.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats
title_short	Bioactive strawberry fruit (Arbutus unedo L.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats
title_full	Bioactive strawberry fruit (Arbutus unedo L.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats
title_fullStr	Bioactive strawberry fruit (Arbutus unedo L.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats
title_full_unstemmed	Bioactive strawberry fruit (Arbutus unedo L.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats
title_sort	bioactive strawberry fruit (arbutus unedo l.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats
publisher	Frontiers Media S.A.
publishDate	2023
url	http://irep.iium.edu.my/107435/2/107435_Bioactive%20strawberry%20fruit%20%28Arbutus%20unedo%20L.%29.pdf http://irep.iium.edu.my/107435/8/107435_Bioactive%20strawberry%20fruit%20%28Arbutus%20unedo%20L.%29_Scopus.pdf http://irep.iium.edu.my/107435/ https://www.frontiersin.org/articles/10.3389/fnins.2023.1244603/full https://doi.org/10.3389/fnins.2023.1244603
_version_	1784511210682580992
score	13.211869

Bioactive strawberry fruit (Arbutus unedo L.) extract remedies paraquat-induced neurotoxicity in the offspring prenatally exposed rats

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