Staff View: A panel of three microRNA signatures as a potential biomarker for CRC screening based on stages and functional prediction using bioinformatic analysis

A panel of three microRNA signatures as a potential biomarker for CRC screening based on stages and functional prediction using bioinformatic analysis

Background: MicroRNA (miRNA) has been linked to colorectal cancer (CRC) tumorigenesis due to its post-transcriptional mechanism in targeting cancer-associated genes. Although miRNAs appear to be promising screening biomarkers, functional prediction analysis is required to shed light on their role in...

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Main Authors:	Mohamed, Syarah Syamimi, Ahmad, Azmir, Ab Mutalib, Nurul Syakima, Tengku Din, Tengku Ahmad Damitri Al-Astani, Md Salleh, Md Salzihan, Zakaria, Andee Dzulkarnaen, Zakaria, Zaidi
Format:	Article
Language:	English English English
Published:	HH Publisher 2023
Subjects:	RC254 Neoplasms. Tumors. Oncology (including Cancer) RC270 Neoplasms. Tumors. Oncology-Cancer and Other Malignant Neoplasms-Therapeutics
Online Access:	http://irep.iium.edu.my/106607/13/106607_A%20panel%20of%20three%20microRNA%20signatures%20as%20a%20potential%20biomarker.pdf http://irep.iium.edu.my/106607/14/106607_A%20panel%20of%20three%20microRNA%20signatures%20as%20a%20potential%20biomarker_Scopus.pdf http://irep.iium.edu.my/106607/25/106607_A%20panel%20of%20three%20microRNA%20signatures%20as%20a%20potential%20biomarker_Research%20materials.pdf http://irep.iium.edu.my/106607/ https://hh-publisher.com/ojs321/index.php/pmmb/article/view/850/443
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spelling	my.iium.irep.1066072023-09-14T08:54:22Z http://irep.iium.edu.my/106607/ A panel of three microRNA signatures as a potential biomarker for CRC screening based on stages and functional prediction using bioinformatic analysis Mohamed, Syarah Syamimi Ahmad, Azmir Ab Mutalib, Nurul Syakima Tengku Din, Tengku Ahmad Damitri Al-Astani Md Salleh, Md Salzihan Zakaria, Andee Dzulkarnaen Zakaria, Zaidi RC254 Neoplasms. Tumors. Oncology (including Cancer) RC270 Neoplasms. Tumors. Oncology-Cancer and Other Malignant Neoplasms-Therapeutics Background: MicroRNA (miRNA) has been linked to colorectal cancer (CRC) tumorigenesis due to its post-transcriptional mechanism in targeting cancer-associated genes. Although miRNAs appear to be promising screening biomarkers, functional prediction analysis is required to shed light on their role in CRC tumorigenesis. Therefore, this study aims to identify the significantly deregulated miRNAs in CRC tumorigenesis. (2) Methods: Three upregulated miRNAs (hsa-miR-20a-5p, hsa-miR-21-5p, and hsa-miR210-3p) from 14 significant differentially expressed miRNAs (DEMs) were chosen from microarray profiling to be validated in plasma. Bioinformatics analyses showed that these miRNAs generally contributed to tumorigenesis, but only hsa-miR-20a-5p and hsa-miR-21-5p were specifically linked to CRC. Only two miRNAs showed a positive correlation when compared to their expression in plasma. However, further analysis showed that all three miRNAs in plasma were significantly difference between the early and advanced stages of CRC. ROC curve analysis was used to evaluate miRNAs’ diagnostic performance in the early and advanced stages. (3) Results: Collectively, hsa-miR-20a-5p showed the highest discriminative value (AUC= 0.82, sensitivity = 86%, and specificity= 88%) in discriminating early CRC, while both hsa-miR-21-5p and hsa-miR-210-3p give a perfect performance for advance CRC. In addition, the performance of all miRNAs’ combinations also gives a perfect performance for diagnosis in both early and advanced CRC, except the combination of hsa-miR-20a-5p and hsa-miR-210-3p. (4) Conclusions: A few potential miRNA panels as CRC biomarker is needed for better prediction of disease. The reflective circulating miRNAs can be contributed to by minimal invasive screening tools. HH Publisher 2023-08-31 Article PeerReviewed application/pdf en http://irep.iium.edu.my/106607/13/106607_A%20panel%20of%20three%20microRNA%20signatures%20as%20a%20potential%20biomarker.pdf application/pdf en http://irep.iium.edu.my/106607/14/106607_A%20panel%20of%20three%20microRNA%20signatures%20as%20a%20potential%20biomarker_Scopus.pdf application/pdf en http://irep.iium.edu.my/106607/25/106607_A%20panel%20of%20three%20microRNA%20signatures%20as%20a%20potential%20biomarker_Research%20materials.pdf Mohamed, Syarah Syamimi and Ahmad, Azmir and Ab Mutalib, Nurul Syakima and Tengku Din, Tengku Ahmad Damitri Al-Astani and Md Salleh, Md Salzihan and Zakaria, Andee Dzulkarnaen and Zakaria, Zaidi (2023) A panel of three microRNA signatures as a potential biomarker for CRC screening based on stages and functional prediction using bioinformatic analysis. Progress in Microbes and Molecular Biology, 6 (1). pp. 1-17. E-ISSN 2637-1049 https://hh-publisher.com/ojs321/index.php/pmmb/article/view/850/443 10.36877/pmmb.a0000343
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English English
topic	RC254 Neoplasms. Tumors. Oncology (including Cancer) RC270 Neoplasms. Tumors. Oncology-Cancer and Other Malignant Neoplasms-Therapeutics
spellingShingle	RC254 Neoplasms. Tumors. Oncology (including Cancer) RC270 Neoplasms. Tumors. Oncology-Cancer and Other Malignant Neoplasms-Therapeutics Mohamed, Syarah Syamimi Ahmad, Azmir Ab Mutalib, Nurul Syakima Tengku Din, Tengku Ahmad Damitri Al-Astani Md Salleh, Md Salzihan Zakaria, Andee Dzulkarnaen Zakaria, Zaidi A panel of three microRNA signatures as a potential biomarker for CRC screening based on stages and functional prediction using bioinformatic analysis
description	Background: MicroRNA (miRNA) has been linked to colorectal cancer (CRC) tumorigenesis due to its post-transcriptional mechanism in targeting cancer-associated genes. Although miRNAs appear to be promising screening biomarkers, functional prediction analysis is required to shed light on their role in CRC tumorigenesis. Therefore, this study aims to identify the significantly deregulated miRNAs in CRC tumorigenesis. (2) Methods: Three upregulated miRNAs (hsa-miR-20a-5p, hsa-miR-21-5p, and hsa-miR210-3p) from 14 significant differentially expressed miRNAs (DEMs) were chosen from microarray profiling to be validated in plasma. Bioinformatics analyses showed that these miRNAs generally contributed to tumorigenesis, but only hsa-miR-20a-5p and hsa-miR-21-5p were specifically linked to CRC. Only two miRNAs showed a positive correlation when compared to their expression in plasma. However, further analysis showed that all three miRNAs in plasma were significantly difference between the early and advanced stages of CRC. ROC curve analysis was used to evaluate miRNAs’ diagnostic performance in the early and advanced stages. (3) Results: Collectively, hsa-miR-20a-5p showed the highest discriminative value (AUC= 0.82, sensitivity = 86%, and specificity= 88%) in discriminating early CRC, while both hsa-miR-21-5p and hsa-miR-210-3p give a perfect performance for advance CRC. In addition, the performance of all miRNAs’ combinations also gives a perfect performance for diagnosis in both early and advanced CRC, except the combination of hsa-miR-20a-5p and hsa-miR-210-3p. (4) Conclusions: A few potential miRNA panels as CRC biomarker is needed for better prediction of disease. The reflective circulating miRNAs can be contributed to by minimal invasive screening tools.
format	Article
author	Mohamed, Syarah Syamimi Ahmad, Azmir Ab Mutalib, Nurul Syakima Tengku Din, Tengku Ahmad Damitri Al-Astani Md Salleh, Md Salzihan Zakaria, Andee Dzulkarnaen Zakaria, Zaidi
author_facet	Mohamed, Syarah Syamimi Ahmad, Azmir Ab Mutalib, Nurul Syakima Tengku Din, Tengku Ahmad Damitri Al-Astani Md Salleh, Md Salzihan Zakaria, Andee Dzulkarnaen Zakaria, Zaidi
author_sort	Mohamed, Syarah Syamimi
title	A panel of three microRNA signatures as a potential biomarker for CRC screening based on stages and functional prediction using bioinformatic analysis
title_short	A panel of three microRNA signatures as a potential biomarker for CRC screening based on stages and functional prediction using bioinformatic analysis
title_full	A panel of three microRNA signatures as a potential biomarker for CRC screening based on stages and functional prediction using bioinformatic analysis
title_fullStr	A panel of three microRNA signatures as a potential biomarker for CRC screening based on stages and functional prediction using bioinformatic analysis
title_full_unstemmed	A panel of three microRNA signatures as a potential biomarker for CRC screening based on stages and functional prediction using bioinformatic analysis
title_sort	panel of three microrna signatures as a potential biomarker for crc screening based on stages and functional prediction using bioinformatic analysis
publisher	HH Publisher
publishDate	2023
url	http://irep.iium.edu.my/106607/13/106607_A%20panel%20of%20three%20microRNA%20signatures%20as%20a%20potential%20biomarker.pdf http://irep.iium.edu.my/106607/14/106607_A%20panel%20of%20three%20microRNA%20signatures%20as%20a%20potential%20biomarker_Scopus.pdf http://irep.iium.edu.my/106607/25/106607_A%20panel%20of%20three%20microRNA%20signatures%20as%20a%20potential%20biomarker_Research%20materials.pdf http://irep.iium.edu.my/106607/ https://hh-publisher.com/ojs321/index.php/pmmb/article/view/850/443
_version_	1778160479995166720
score	13.211869

A panel of three microRNA signatures as a potential biomarker for CRC screening based on stages and functional prediction using bioinformatic analysis

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