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Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry

Problem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a generic factor rather than environmental or intrinsic factor...

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Main Authors: Mahdi, Harith Jameel, Budi Muljono, Retno Andayani, -, Ishak
Format: Article
Language:English
Published: Science Publications 2010
Subjects:
Q Science (General)
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/10627/1/PUB2.PDF
http://irep.iium.edu.my/10627/
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spelling my.iium.irep.106272012-01-13T09:21:20Z http://irep.iium.edu.my/10627/ Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry Mahdi, Harith Jameel Budi Muljono, Retno Andayani -, Ishak Q Science (General) RS Pharmacy and materia medica Problem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a generic factor rather than environmental or intrinsic factors. Approach: A comprehensive metabolic fingerprinting from the leaves of three micro-propagated ginger cultivars Bukit Tinggi, Tanjung Sepat and Sabah was performed using a Gas Chromatography-Mass Spectrometry (GC-MS). Constituents of the ginger leaves were first extracted and then fractionated into methanolic and chloroform. The samples were subsequently methoximated and silylated prior to GC-MS analysis. Results: By applying this technique, we detected more than 300 compounds (polar and non-polar) in total originated from each ginger cultivars. Based on the GC-MS fragmentation, three different classes of metabolites were detected from the ginger cultivars, namely amino acids, carbohydrates and organic acids. A qualitative variation on the type of ginger metabolites was observed, albeit no marked different found in the level of the metabolites. Conclusion: Apparently the chemical variations among the three ginger cultivars were due to genetic effects since almost all other environmental and intrinsic factors were eliminated. Science Publications 2010 Article REM application/pdf en http://irep.iium.edu.my/10627/1/PUB2.PDF Mahdi, Harith Jameel and Budi Muljono, Retno Andayani and -, Ishak (2010) Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry. American Journal of Applied Science, 7 (1). pp. 17-23. ISSN 1546-9239
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
RS Pharmacy and materia medica
spellingShingle Q Science (General)
RS Pharmacy and materia medica
Mahdi, Harith Jameel
Budi Muljono, Retno Andayani
-, Ishak
Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
description Problem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a generic factor rather than environmental or intrinsic factors. Approach: A comprehensive metabolic fingerprinting from the leaves of three micro-propagated ginger cultivars Bukit Tinggi, Tanjung Sepat and Sabah was performed using a Gas Chromatography-Mass Spectrometry (GC-MS). Constituents of the ginger leaves were first extracted and then fractionated into methanolic and chloroform. The samples were subsequently methoximated and silylated prior to GC-MS analysis. Results: By applying this technique, we detected more than 300 compounds (polar and non-polar) in total originated from each ginger cultivars. Based on the GC-MS fragmentation, three different classes of metabolites were detected from the ginger cultivars, namely amino acids, carbohydrates and organic acids. A qualitative variation on the type of ginger metabolites was observed, albeit no marked different found in the level of the metabolites. Conclusion: Apparently the chemical variations among the three ginger cultivars were due to genetic effects since almost all other environmental and intrinsic factors were eliminated.
format Article
author Mahdi, Harith Jameel
Budi Muljono, Retno Andayani
-, Ishak
author_facet Mahdi, Harith Jameel
Budi Muljono, Retno Andayani
-, Ishak
author_sort Mahdi, Harith Jameel
title Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
title_short Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
title_full Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
title_fullStr Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
title_full_unstemmed Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
title_sort metabolic fingerprinting of three malaysian ginger (zingiber officinale roscoe) using gas chromatography-mass spectromertry
publisher Science Publications
publishDate 2010
url http://irep.iium.edu.my/10627/1/PUB2.PDF
http://irep.iium.edu.my/10627/
_version_ 1643606394099728384
score 13.211869

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