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Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications

Background: Plasmid-mediated quinolone-resistant (PMQR) genes and mutations within the quinolone resistance determining regions (QRDRs) resulted in the advent of quinolone-resistant pathogenic microbes. This research was designed to assess the roles of three PMQR genes, qnrA, qnrB, and qnrS, and a...

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Main Authors: Haque, Tanjum Ara, Urmi, Umme Laila, Md Khademul Islam, Abul Bashar Mir, Ara, Bayasrin, Nahar, Shamsun, Md Mosaddek, Abu Syed, Lugova, Halyna, Kumar, Santosh, Jahan, Dilshad, A. Rahman, Nor Azlina, Haque, Mainul, Islam, Salequl, Godman, Brian
Format: Article
Language:English
English
Published: Open Science Publishers 2022
Subjects:
R Medicine (General)
RZ Other systems of medicine
Online Access:http://irep.iium.edu.my/106030/7/106030_Detection%20of%20qnr%20genes%20and%20gyrA%20mutation%20to%20quinolone.pdf
http://irep.iium.edu.my/106030/13/106030_Detection%20of%20qnr%20genes%20and%20gyrA%20mutation%20to%20quinolone_SCOPUS.pdf
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https://japsonline.com/abstract.php?article_id=3614&sts=2
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spelling my.iium.irep.1060302023-08-17T08:59:42Z http://irep.iium.edu.my/106030/ Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications Haque, Tanjum Ara Urmi, Umme Laila Md Khademul Islam, Abul Bashar Mir Ara, Bayasrin Nahar, Shamsun Md Mosaddek, Abu Syed Lugova, Halyna Kumar, Santosh Jahan, Dilshad A. Rahman, Nor Azlina Haque, Mainul Islam, Salequl Godman, Brian R Medicine (General) RZ Other systems of medicine Background: Plasmid-mediated quinolone-resistant (PMQR) genes and mutations within the quinolone resistance determining regions (QRDRs) resulted in the advent of quinolone-resistant pathogenic microbes. This research was designed to assess the roles of three PMQR genes, qnrA, qnrB, and qnrS, and any mutation in the gyrA gene in the QRDR as a process of quinolone/fluoroquinolone resistance to urinary tract infection (UTI) bacteria in Bangladesh to guide future management of UTIs. Methods: Pathogens from UTIs were isolated and identified, and their phenotype antibiotic susceptibilities were tested for lomefloxacin, ofloxacin, ciprofloxacin, and nalidixic acid. Polymerase chain reaction (PCR) detected the qnrA, qnrB, and qnrS genes. PCR and sequencing were performed to evaluate any mutation within the QRDRs of the gyrA gene. Results: Of 100 UTI bacteria, phenotypic resistance was observed in 95.0%, 89.0%, 83.0%, and 71.0% against lomefloxacin, nalidixic acid, ofloxacin, and ciprofloxacin, respectively. PMQR genes qnrS, qnrA, and qnrB genes were found in 54.0%, 1.0%, and 4.0% of isolates, respectively. Sequencing the gyrA gene revealed single mutation (Ser-83 to Leu) and double mutations (Ser-83 to Leu and Asp-87 to Asn). PMQR genes showed a statistically nonsignificant association with phenotypic resistance. Conclusions: This study confirms the presence of QRDR mutations that were independent of PMQR genes. Consequently, high resistance against quinolones among uropathogens is evident, and their future use needs to be moderated. Open Science Publishers 2022-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/106030/7/106030_Detection%20of%20qnr%20genes%20and%20gyrA%20mutation%20to%20quinolone.pdf application/pdf en http://irep.iium.edu.my/106030/13/106030_Detection%20of%20qnr%20genes%20and%20gyrA%20mutation%20to%20quinolone_SCOPUS.pdf Haque, Tanjum Ara and Urmi, Umme Laila and Md Khademul Islam, Abul Bashar Mir and Ara, Bayasrin and Nahar, Shamsun and Md Mosaddek, Abu Syed and Lugova, Halyna and Kumar, Santosh and Jahan, Dilshad and A. Rahman, Nor Azlina and Haque, Mainul and Islam, Salequl and Godman, Brian (2022) Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications. Journal of Applied Pharmaceutical Science, 12 (4). pp. 185-198. ISSN 2231-3354 https://japsonline.com/abstract.php?article_id=3614&sts=2 10.7324/JAPS.2022.120422
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic R Medicine (General)
RZ Other systems of medicine
spellingShingle R Medicine (General)
RZ Other systems of medicine
Haque, Tanjum Ara
Urmi, Umme Laila
Md Khademul Islam, Abul Bashar Mir
Ara, Bayasrin
Nahar, Shamsun
Md Mosaddek, Abu Syed
Lugova, Halyna
Kumar, Santosh
Jahan, Dilshad
A. Rahman, Nor Azlina
Haque, Mainul
Islam, Salequl
Godman, Brian
Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications
description Background: Plasmid-mediated quinolone-resistant (PMQR) genes and mutations within the quinolone resistance determining regions (QRDRs) resulted in the advent of quinolone-resistant pathogenic microbes. This research was designed to assess the roles of three PMQR genes, qnrA, qnrB, and qnrS, and any mutation in the gyrA gene in the QRDR as a process of quinolone/fluoroquinolone resistance to urinary tract infection (UTI) bacteria in Bangladesh to guide future management of UTIs. Methods: Pathogens from UTIs were isolated and identified, and their phenotype antibiotic susceptibilities were tested for lomefloxacin, ofloxacin, ciprofloxacin, and nalidixic acid. Polymerase chain reaction (PCR) detected the qnrA, qnrB, and qnrS genes. PCR and sequencing were performed to evaluate any mutation within the QRDRs of the gyrA gene. Results: Of 100 UTI bacteria, phenotypic resistance was observed in 95.0%, 89.0%, 83.0%, and 71.0% against lomefloxacin, nalidixic acid, ofloxacin, and ciprofloxacin, respectively. PMQR genes qnrS, qnrA, and qnrB genes were found in 54.0%, 1.0%, and 4.0% of isolates, respectively. Sequencing the gyrA gene revealed single mutation (Ser-83 to Leu) and double mutations (Ser-83 to Leu and Asp-87 to Asn). PMQR genes showed a statistically nonsignificant association with phenotypic resistance. Conclusions: This study confirms the presence of QRDR mutations that were independent of PMQR genes. Consequently, high resistance against quinolones among uropathogens is evident, and their future use needs to be moderated.
format Article
author Haque, Tanjum Ara
Urmi, Umme Laila
Md Khademul Islam, Abul Bashar Mir
Ara, Bayasrin
Nahar, Shamsun
Md Mosaddek, Abu Syed
Lugova, Halyna
Kumar, Santosh
Jahan, Dilshad
A. Rahman, Nor Azlina
Haque, Mainul
Islam, Salequl
Godman, Brian
author_facet Haque, Tanjum Ara
Urmi, Umme Laila
Md Khademul Islam, Abul Bashar Mir
Ara, Bayasrin
Nahar, Shamsun
Md Mosaddek, Abu Syed
Lugova, Halyna
Kumar, Santosh
Jahan, Dilshad
A. Rahman, Nor Azlina
Haque, Mainul
Islam, Salequl
Godman, Brian
author_sort Haque, Tanjum Ara
title Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications
title_short Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications
title_full Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications
title_fullStr Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications
title_full_unstemmed Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications
title_sort detection of qnr genes and gyra mutation to quinolone phenotypic resistance of uti pathogens in bangladesh and the implications
publisher Open Science Publishers
publishDate 2022
url http://irep.iium.edu.my/106030/7/106030_Detection%20of%20qnr%20genes%20and%20gyrA%20mutation%20to%20quinolone.pdf
http://irep.iium.edu.my/106030/13/106030_Detection%20of%20qnr%20genes%20and%20gyrA%20mutation%20to%20quinolone_SCOPUS.pdf
http://irep.iium.edu.my/106030/
https://japsonline.com/abstract.php?article_id=3614&sts=2
_version_ 1775621723011416064
score 13.211869

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