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The co-isolation of Lactic Acid Bacteria (LAB) and a related pathogenic strain from Pangasius nasutus

Catfish species Pangasius nasutus, or locally known as Patin Buah is one of a popular aquaculture product in Malaysia. Probiotic strain is an attractive alternative to conventional antibiotics in managing aquaculture diseases. Pangasius can be a source of bacterial strain in developing suitable prob...

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Main Authors: Haja Mohideen, Nur Hannah Rashidah, Alias, Nurul Sakinah, Mansor, Nur Nazifah, Tengku Abdul Hamid, Tengku Haziyamin
Format: Article
Language:English
Published: International Society of Academicians 2023
Subjects:
QR Microbiology
S Agriculture (General)
SH Aquaculture. Fisheries. Angling
Online Access:http://irep.iium.edu.my/105875/1/105875_The%20co-Isolation%20of%20Lactic%20Acid%20Bacteria.pdf
http://irep.iium.edu.my/105875/
https://dergipark.org.tr/en/pub/ijlsb/issue/79203/1268388
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spelling my.iium.irep.1058752023-08-08T02:41:20Z http://irep.iium.edu.my/105875/ The co-isolation of Lactic Acid Bacteria (LAB) and a related pathogenic strain from Pangasius nasutus Haja Mohideen, Nur Hannah Rashidah Alias, Nurul Sakinah Mansor, Nur Nazifah Tengku Abdul Hamid, Tengku Haziyamin QR Microbiology S Agriculture (General) SH Aquaculture. Fisheries. Angling Catfish species Pangasius nasutus, or locally known as Patin Buah is one of a popular aquaculture product in Malaysia. Probiotic strain is an attractive alternative to conventional antibiotics in managing aquaculture diseases. Pangasius can be a source of bacterial strain in developing suitable probiotic useful in Pangasius rearing. This study highlights on the isolation of Lactic Acid Bacteria (LAB) strains from the selected tissues from P. nasutus which are the heart, stomach, and intestines. These strains were subjected to morphological, biochemical, and genotypic characterisations. Three different strains were isolated from fish tissues, H-Hn from heart; S-Hn from stomach; and I-Sk from intestine. Biochemical characterisation were consistent with ribosomal rRNA sequencing, in which strain H-Hn (acc. No. MW504962) is highly similar (100%) to Lactococcus lactis, and both I-Sk (MW504964) and S-Hn (MW504963) are similar (99%) to Lactococcus garvieae. All strains appeared to be non-spore forming, non-motile, Gram-positive coccus, catalase and citrate negative, and fermenting sugar. Unlike H-Hn, both H-Sk and I-Sk strains were able to grow at 6.5% NaCl and at higher temperature (45°C). The antimicrobial properties were assessed using agar disk diffusion assay against several indicator organisms (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus and Vibrio parahaemolyticus). These strains exhibited varying degree of inhibitory properties against the indicator organisms. Strain H-Hn inhibited E. coli and V. parahaemolyticus. Meanwhile, strains I-Sk and S-Hn showed a rather broad-spectrum inhibition. From P. nasutus, other L. lactis strain, a pathogenic species from L garvieae could also be found especially in their gut tissues. The presence of L. garvieae in commercial P. nasutus should raise some concern to those who like to consume this fish. Meanwhile, the LAB strains isolated has probiotics potential that can be commercially used in the managing fish diseases in aquaculture. International Society of Academicians 2023-07-30 Article PeerReviewed application/pdf en http://irep.iium.edu.my/105875/1/105875_The%20co-Isolation%20of%20Lactic%20Acid%20Bacteria.pdf Haja Mohideen, Nur Hannah Rashidah and Alias, Nurul Sakinah and Mansor, Nur Nazifah and Tengku Abdul Hamid, Tengku Haziyamin (2023) The co-isolation of Lactic Acid Bacteria (LAB) and a related pathogenic strain from Pangasius nasutus. International Journal of Life Sciences and Biotechnology, 6 (2). pp. 143-154. E-ISSN 2651-4621 https://dergipark.org.tr/en/pub/ijlsb/issue/79203/1268388 10.38001/ijlsb.1268388
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QR Microbiology
S Agriculture (General)
SH Aquaculture. Fisheries. Angling
spellingShingle QR Microbiology
S Agriculture (General)
SH Aquaculture. Fisheries. Angling
Haja Mohideen, Nur Hannah Rashidah
Alias, Nurul Sakinah
Mansor, Nur Nazifah
Tengku Abdul Hamid, Tengku Haziyamin
The co-isolation of Lactic Acid Bacteria (LAB) and a related pathogenic strain from Pangasius nasutus
description Catfish species Pangasius nasutus, or locally known as Patin Buah is one of a popular aquaculture product in Malaysia. Probiotic strain is an attractive alternative to conventional antibiotics in managing aquaculture diseases. Pangasius can be a source of bacterial strain in developing suitable probiotic useful in Pangasius rearing. This study highlights on the isolation of Lactic Acid Bacteria (LAB) strains from the selected tissues from P. nasutus which are the heart, stomach, and intestines. These strains were subjected to morphological, biochemical, and genotypic characterisations. Three different strains were isolated from fish tissues, H-Hn from heart; S-Hn from stomach; and I-Sk from intestine. Biochemical characterisation were consistent with ribosomal rRNA sequencing, in which strain H-Hn (acc. No. MW504962) is highly similar (100%) to Lactococcus lactis, and both I-Sk (MW504964) and S-Hn (MW504963) are similar (99%) to Lactococcus garvieae. All strains appeared to be non-spore forming, non-motile, Gram-positive coccus, catalase and citrate negative, and fermenting sugar. Unlike H-Hn, both H-Sk and I-Sk strains were able to grow at 6.5% NaCl and at higher temperature (45°C). The antimicrobial properties were assessed using agar disk diffusion assay against several indicator organisms (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus and Vibrio parahaemolyticus). These strains exhibited varying degree of inhibitory properties against the indicator organisms. Strain H-Hn inhibited E. coli and V. parahaemolyticus. Meanwhile, strains I-Sk and S-Hn showed a rather broad-spectrum inhibition. From P. nasutus, other L. lactis strain, a pathogenic species from L garvieae could also be found especially in their gut tissues. The presence of L. garvieae in commercial P. nasutus should raise some concern to those who like to consume this fish. Meanwhile, the LAB strains isolated has probiotics potential that can be commercially used in the managing fish diseases in aquaculture.
format Article
author Haja Mohideen, Nur Hannah Rashidah
Alias, Nurul Sakinah
Mansor, Nur Nazifah
Tengku Abdul Hamid, Tengku Haziyamin
author_facet Haja Mohideen, Nur Hannah Rashidah
Alias, Nurul Sakinah
Mansor, Nur Nazifah
Tengku Abdul Hamid, Tengku Haziyamin
author_sort Haja Mohideen, Nur Hannah Rashidah
title The co-isolation of Lactic Acid Bacteria (LAB) and a related pathogenic strain from Pangasius nasutus
title_short The co-isolation of Lactic Acid Bacteria (LAB) and a related pathogenic strain from Pangasius nasutus
title_full The co-isolation of Lactic Acid Bacteria (LAB) and a related pathogenic strain from Pangasius nasutus
title_fullStr The co-isolation of Lactic Acid Bacteria (LAB) and a related pathogenic strain from Pangasius nasutus
title_full_unstemmed The co-isolation of Lactic Acid Bacteria (LAB) and a related pathogenic strain from Pangasius nasutus
title_sort co-isolation of lactic acid bacteria (lab) and a related pathogenic strain from pangasius nasutus
publisher International Society of Academicians
publishDate 2023
url http://irep.iium.edu.my/105875/1/105875_The%20co-Isolation%20of%20Lactic%20Acid%20Bacteria.pdf
http://irep.iium.edu.my/105875/
https://dergipark.org.tr/en/pub/ijlsb/issue/79203/1268388
_version_ 1775621711699378176
score 13.211869

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