Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM)
Mangosteen (Garcinia mangostana var. mangostana) is one of the common tropical fruits in South East Asia. The value and economic potential of mangosteen have been recognised by local and international markets due to its fleshy part of the pulp that can be eaten fresh as well as medicinal properties...
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Persatuan Genetik Malaysia
2022
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my.iium.irep.1050332023-06-08T02:46:00Z http://irep.iium.edu.my/105033/ Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM) Shaharudin, Mohamad Azimi Emlee, Aiesyaa Majdiena Midin, Mohd Razik QH426 Genetics Mangosteen (Garcinia mangostana var. mangostana) is one of the common tropical fruits in South East Asia. The value and economic potential of mangosteen have been recognised by local and international markets due to its fleshy part of the pulp that can be eaten fresh as well as medicinal properties including anti-inflammatory and anti-oxidative characteristics. Mangosteen grows best in fertile soil with slightly acidic conditions and can only be found in cultivated farms especially in few countries including Thailand, Malaysia, Philippines, and Indonesia. In order to improve both quality and quantity of mangosteen cultivation, several Garcinia species have been identified as possible ancestors of mangosteen which can be used in breeding program. Currently, the genome size on these possible ancestors are still unavailable. This study aims to determine the genome size of two Garcinia species of possible mangosteen ancestors; G. opaca and G. penangiana using flow cytometry (FCM) analysis. By using soybean or Glycine max cv. Polanka (2C=2.5pg) as an external reference standard, it was found that the genome size of G. opaca and G. penangiana to be 3.34±0.05 pg and 4.77±0.31* pg respectively. Recent study proved that the genome size of mangosteen to be 6.00±0.17 pg which was higher than the genome size of both G. opaca and G. penangiana. The findings indicate that interspecific variation which resulted from hybridization process between G. opaca and G. penangiana may occur in the genome of mangosteen. In conclusion, cytological data such as genome size for Garcinia species can be utilised for further study to determine the origin of mangosteen. Persatuan Genetik Malaysia 2022-12-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/105033/7/105033_Genome%20size%20determination%20of%20garcinia%20opaca.pdf Shaharudin, Mohamad Azimi and Emlee, Aiesyaa Majdiena and Midin, Mohd Razik (2022) Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM). Malaysian Journal of Genetics, 2 (1). pp. 29-36. E-ISSN 2811-3594 https://drive.google.com/file/d/1h1LNOvvhR6d6xnGioLvlDx4fh7-ngfPt/view?usp=share_link |
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QH426 Genetics Shaharudin, Mohamad Azimi Emlee, Aiesyaa Majdiena Midin, Mohd Razik Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM) |
| description |
Mangosteen (Garcinia mangostana var. mangostana) is one of the common tropical fruits in South East Asia. The value and economic potential of mangosteen have been recognised by local and international markets due to its fleshy part of the pulp that can be eaten fresh as well as medicinal properties including anti-inflammatory and anti-oxidative characteristics. Mangosteen grows best in fertile soil with slightly acidic conditions and can only be found in cultivated farms especially in few countries including Thailand, Malaysia, Philippines, and Indonesia. In order to improve both quality and quantity of mangosteen cultivation, several Garcinia species have been identified as possible ancestors of mangosteen which can be used in breeding program. Currently, the genome size on
these possible ancestors are still unavailable. This study aims to determine the genome size of two Garcinia species of possible mangosteen ancestors; G. opaca and G. penangiana using flow
cytometry (FCM) analysis. By using soybean or Glycine max cv. Polanka (2C=2.5pg) as an external reference standard, it was found that the genome size of G. opaca and G. penangiana to be
3.34±0.05 pg and 4.77±0.31* pg respectively. Recent study proved that the genome size of mangosteen to be 6.00±0.17 pg which was higher than the genome size of both G. opaca and G.
penangiana. The findings indicate that interspecific variation which resulted from hybridization process between G. opaca and G. penangiana may occur in the genome of mangosteen. In
conclusion, cytological data such as genome size for Garcinia species can be utilised for further study to determine the origin of mangosteen. |
| format |
Article |
| author |
Shaharudin, Mohamad Azimi Emlee, Aiesyaa Majdiena Midin, Mohd Razik |
| author_facet |
Shaharudin, Mohamad Azimi Emlee, Aiesyaa Majdiena Midin, Mohd Razik |
| author_sort |
Shaharudin, Mohamad Azimi |
| title |
Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM) |
| title_short |
Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM) |
| title_full |
Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM) |
| title_fullStr |
Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM) |
| title_full_unstemmed |
Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM) |
| title_sort |
genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (fcm) |
| publisher |
Persatuan Genetik Malaysia |
| publishDate |
2022 |
| url |
http://irep.iium.edu.my/105033/7/105033_Genome%20size%20determination%20of%20garcinia%20opaca.pdf http://irep.iium.edu.my/105033/ https://drive.google.com/file/d/1h1LNOvvhR6d6xnGioLvlDx4fh7-ngfPt/view?usp=share_link |
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1769841825063895040 |
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13.250246 |