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Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM)

Mangosteen (Garcinia mangostana var. mangostana) is one of the common tropical fruits in South East Asia. The value and economic potential of mangosteen have been recognised by local and international markets due to its fleshy part of the pulp that can be eaten fresh as well as medicinal properties...

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Main Authors: Shaharudin, Mohamad Azimi, Emlee, Aiesyaa Majdiena, Midin, Mohd Razik
Format: Article
Language:English
Published: Persatuan Genetik Malaysia 2022
Subjects:
QH426 Genetics
Online Access:http://irep.iium.edu.my/105033/7/105033_Genome%20size%20determination%20of%20garcinia%20opaca.pdf
http://irep.iium.edu.my/105033/
https://drive.google.com/file/d/1h1LNOvvhR6d6xnGioLvlDx4fh7-ngfPt/view?usp=share_link
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spelling my.iium.irep.1050332023-06-08T02:46:00Z http://irep.iium.edu.my/105033/ Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM) Shaharudin, Mohamad Azimi Emlee, Aiesyaa Majdiena Midin, Mohd Razik QH426 Genetics Mangosteen (Garcinia mangostana var. mangostana) is one of the common tropical fruits in South East Asia. The value and economic potential of mangosteen have been recognised by local and international markets due to its fleshy part of the pulp that can be eaten fresh as well as medicinal properties including anti-inflammatory and anti-oxidative characteristics. Mangosteen grows best in fertile soil with slightly acidic conditions and can only be found in cultivated farms especially in few countries including Thailand, Malaysia, Philippines, and Indonesia. In order to improve both quality and quantity of mangosteen cultivation, several Garcinia species have been identified as possible ancestors of mangosteen which can be used in breeding program. Currently, the genome size on these possible ancestors are still unavailable. This study aims to determine the genome size of two Garcinia species of possible mangosteen ancestors; G. opaca and G. penangiana using flow cytometry (FCM) analysis. By using soybean or Glycine max cv. Polanka (2C=2.5pg) as an external reference standard, it was found that the genome size of G. opaca and G. penangiana to be 3.34±0.05 pg and 4.77±0.31* pg respectively. Recent study proved that the genome size of mangosteen to be 6.00±0.17 pg which was higher than the genome size of both G. opaca and G. penangiana. The findings indicate that interspecific variation which resulted from hybridization process between G. opaca and G. penangiana may occur in the genome of mangosteen. In conclusion, cytological data such as genome size for Garcinia species can be utilised for further study to determine the origin of mangosteen. Persatuan Genetik Malaysia 2022-12-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/105033/7/105033_Genome%20size%20determination%20of%20garcinia%20opaca.pdf Shaharudin, Mohamad Azimi and Emlee, Aiesyaa Majdiena and Midin, Mohd Razik (2022) Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM). Malaysian Journal of Genetics, 2 (1). pp. 29-36. E-ISSN 2811-3594 https://drive.google.com/file/d/1h1LNOvvhR6d6xnGioLvlDx4fh7-ngfPt/view?usp=share_link
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QH426 Genetics
spellingShingle QH426 Genetics
Shaharudin, Mohamad Azimi
Emlee, Aiesyaa Majdiena
Midin, Mohd Razik
Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM)
description Mangosteen (Garcinia mangostana var. mangostana) is one of the common tropical fruits in South East Asia. The value and economic potential of mangosteen have been recognised by local and international markets due to its fleshy part of the pulp that can be eaten fresh as well as medicinal properties including anti-inflammatory and anti-oxidative characteristics. Mangosteen grows best in fertile soil with slightly acidic conditions and can only be found in cultivated farms especially in few countries including Thailand, Malaysia, Philippines, and Indonesia. In order to improve both quality and quantity of mangosteen cultivation, several Garcinia species have been identified as possible ancestors of mangosteen which can be used in breeding program. Currently, the genome size on these possible ancestors are still unavailable. This study aims to determine the genome size of two Garcinia species of possible mangosteen ancestors; G. opaca and G. penangiana using flow cytometry (FCM) analysis. By using soybean or Glycine max cv. Polanka (2C=2.5pg) as an external reference standard, it was found that the genome size of G. opaca and G. penangiana to be 3.34±0.05 pg and 4.77±0.31* pg respectively. Recent study proved that the genome size of mangosteen to be 6.00±0.17 pg which was higher than the genome size of both G. opaca and G. penangiana. The findings indicate that interspecific variation which resulted from hybridization process between G. opaca and G. penangiana may occur in the genome of mangosteen. In conclusion, cytological data such as genome size for Garcinia species can be utilised for further study to determine the origin of mangosteen.
format Article
author Shaharudin, Mohamad Azimi
Emlee, Aiesyaa Majdiena
Midin, Mohd Razik
author_facet Shaharudin, Mohamad Azimi
Emlee, Aiesyaa Majdiena
Midin, Mohd Razik
author_sort Shaharudin, Mohamad Azimi
title Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM)
title_short Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM)
title_full Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM)
title_fullStr Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM)
title_full_unstemmed Genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (FCM)
title_sort genome size determination of garcinia opaca and garcinia penangiana using flow cytometry (fcm)
publisher Persatuan Genetik Malaysia
publishDate 2022
url http://irep.iium.edu.my/105033/7/105033_Genome%20size%20determination%20of%20garcinia%20opaca.pdf
http://irep.iium.edu.my/105033/
https://drive.google.com/file/d/1h1LNOvvhR6d6xnGioLvlDx4fh7-ngfPt/view?usp=share_link
_version_ 1769841825063895040
score 13.211869

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