Staff View: Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds

Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds

Tissue-engineered polymeric implants are preferable because they do not cause a significant inflammatory reaction in the surrounding tissue. Three-dimensional (3D) technology can be used to fabricate a customised scaffold, which is critical for implantation. This study aimed to investigate the bioco...

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Main Authors:	Abdul Samat, Asmak, Abdul Hamid, Zuratul Ain, Jaafar, Mariatti, Ong, Chern Chung, Yahaya, Badrul Hisham
Format:	Article
Language:	English English
Published:	MDPI 2023
Subjects:	QP Physiology R Medicine (General) T Technology (General) T201 Patents. Trademarks
Online Access:	http://irep.iium.edu.my/104637/2/104637_Investigation%20of%20the%20In%20Vitro%20and%20In%20Vivo%20Biocompatibility.pdf http://irep.iium.edu.my/104637/3/104637_Investigation%20of%20the%20In%20Vitro%20and%20In%20Vivo%20Biocompatibility%20of%20a%20Three-Dimensional%20Printed%20Thermoplastic%20Polyurethane_Scopus.pdf http://irep.iium.edu.my/104637/ https://doi.org/10.3390/bioengineering10040394
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spelling	my.iium.irep.1046372023-05-17T13:29:50Z http://irep.iium.edu.my/104637/ Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds Abdul Samat, Asmak Abdul Hamid, Zuratul Ain Jaafar, Mariatti Ong, Chern Chung Yahaya, Badrul Hisham QP Physiology R Medicine (General) T Technology (General) T201 Patents. Trademarks Tissue-engineered polymeric implants are preferable because they do not cause a significant inflammatory reaction in the surrounding tissue. Three-dimensional (3D) technology can be used to fabricate a customised scaffold, which is critical for implantation. This study aimed to investigate the biocompatibility of a mixture of thermoplastic polyurethane (TPU) and polylactic acid (PLA) and the effects of their extract in cell cultures and in animal models as potential tracheal replacement materials. The morphology of the 3D-printed scaffolds was investigated using scanning electron microscopy (SEM), while the degradability, pH, and effects of the 3D-printed TPU/PLA scaffolds and their extracts were investigated in cell culture studies. In addition, subcutaneous implantation of 3D-printed scaffold was performed to evaluate the biocompatibility of the scaffold in a rat model at different time points. A histopathological examination was performed to investigate the local inflammatory response and angiogenesis. The in vitro results showed that the composite and its extract were not toxic. Similarly, the pH of the extracts did not inhibit cell proliferation and migration. The analysis of biocompatibility of the scaffolds from the in vivo results suggests that porous TPU/PLA scaffolds may facilitate cell adhesion, migration, and proliferation and promote angiogenesis in host cells. The current results suggest that with 3D printing technology, TPU and PLA could be used as materials to construct scaffolds with suitable properties and provide a solution to the challenges of tracheal transplantation. MDPI 2023-03-23 Article PeerReviewed application/pdf en http://irep.iium.edu.my/104637/2/104637_Investigation%20of%20the%20In%20Vitro%20and%20In%20Vivo%20Biocompatibility.pdf application/pdf en http://irep.iium.edu.my/104637/3/104637_Investigation%20of%20the%20In%20Vitro%20and%20In%20Vivo%20Biocompatibility%20of%20a%20Three-Dimensional%20Printed%20Thermoplastic%20Polyurethane_Scopus.pdf Abdul Samat, Asmak and Abdul Hamid, Zuratul Ain and Jaafar, Mariatti and Ong, Chern Chung and Yahaya, Badrul Hisham (2023) Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds. Bioengineering 2023, 10 (394). E-ISSN 2306-5354 https://doi.org/10.3390/bioengineering10040394
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English
topic	QP Physiology R Medicine (General) T Technology (General) T201 Patents. Trademarks
spellingShingle	QP Physiology R Medicine (General) T Technology (General) T201 Patents. Trademarks Abdul Samat, Asmak Abdul Hamid, Zuratul Ain Jaafar, Mariatti Ong, Chern Chung Yahaya, Badrul Hisham Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds
description	Tissue-engineered polymeric implants are preferable because they do not cause a significant inflammatory reaction in the surrounding tissue. Three-dimensional (3D) technology can be used to fabricate a customised scaffold, which is critical for implantation. This study aimed to investigate the biocompatibility of a mixture of thermoplastic polyurethane (TPU) and polylactic acid (PLA) and the effects of their extract in cell cultures and in animal models as potential tracheal replacement materials. The morphology of the 3D-printed scaffolds was investigated using scanning electron microscopy (SEM), while the degradability, pH, and effects of the 3D-printed TPU/PLA scaffolds and their extracts were investigated in cell culture studies. In addition, subcutaneous implantation of 3D-printed scaffold was performed to evaluate the biocompatibility of the scaffold in a rat model at different time points. A histopathological examination was performed to investigate the local inflammatory response and angiogenesis. The in vitro results showed that the composite and its extract were not toxic. Similarly, the pH of the extracts did not inhibit cell proliferation and migration. The analysis of biocompatibility of the scaffolds from the in vivo results suggests that porous TPU/PLA scaffolds may facilitate cell adhesion, migration, and proliferation and promote angiogenesis in host cells. The current results suggest that with 3D printing technology, TPU and PLA could be used as materials to construct scaffolds with suitable properties and provide a solution to the challenges of tracheal transplantation.
format	Article
author	Abdul Samat, Asmak Abdul Hamid, Zuratul Ain Jaafar, Mariatti Ong, Chern Chung Yahaya, Badrul Hisham
author_facet	Abdul Samat, Asmak Abdul Hamid, Zuratul Ain Jaafar, Mariatti Ong, Chern Chung Yahaya, Badrul Hisham
author_sort	Abdul Samat, Asmak
title	Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds
title_short	Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds
title_full	Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds
title_fullStr	Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds
title_full_unstemmed	Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds
title_sort	investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed thermoplastic polyurethane/polylactic acid blend for the development of tracheal scaffolds
publisher	MDPI
publishDate	2023
url	http://irep.iium.edu.my/104637/2/104637_Investigation%20of%20the%20In%20Vitro%20and%20In%20Vivo%20Biocompatibility.pdf http://irep.iium.edu.my/104637/3/104637_Investigation%20of%20the%20In%20Vitro%20and%20In%20Vivo%20Biocompatibility%20of%20a%20Three-Dimensional%20Printed%20Thermoplastic%20Polyurethane_Scopus.pdf http://irep.iium.edu.my/104637/ https://doi.org/10.3390/bioengineering10040394
_version_	1768006374170558464
score	13.211869

Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds

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