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The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region

The growth conditions of bacteria producing cold-active protease isolated from the Antarctic sample were screened using one-factor-at-time (OFAT). Then, crude protease of the strain was extracted during late logarithmic phase for enzymatic assay. A strain that showed the highest enzyme activity was...

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Main Author: Yusof, Faridah
Format: Article
Language:English
Published: 2022
Subjects:
T Technology (General)
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/102082/1/Asyraf%20%5BIIUMEJ%5D%202447_%20Editor%20Decision%20-%20Accept%201818.12.2022.pdf
http://irep.iium.edu.my/102082/
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spelling my.iium.irep.1020822023-02-01T09:33:00Z http://irep.iium.edu.my/102082/ The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region Yusof, Faridah T Technology (General) TP248.13 Biotechnology The growth conditions of bacteria producing cold-active protease isolated from the Antarctic sample were screened using one-factor-at-time (OFAT). Then, crude protease of the strain was extracted during late logarithmic phase for enzymatic assay. A strain that showed the highest enzyme activity was selected for optimization via Response Surface Method (RSM). The parameters studied were incubation temperature (4 - 36 °C), pH media (4 – 10) and NaCl concentration (0 - 8 %). Based on the OFAT results, all eight strains showed the highest growth rate at 20 °C, pH 7 and 4% (w/v) NaCl. The assay showed that the crude enzyme extracted from strain SC8 exhibited significantly higher activity (0.20 U and 0.37 U) than the positive control (0.11 U and 0.31 U) at -20 °C and 20 °C. RSM suggested that the optimized setting for growth of SC8 were at 20.5 °C, pH 6.83 and 2.05% (w/v) of NaCl with the results of the bacterial growth rate value was 3.70 ± 0.06 x 106 cells/hr. Optimal growth conditions of SC8 from this study are useful for the large-scale production of cold-active protease in future. 2022 Article PeerReviewed application/pdf en http://irep.iium.edu.my/102082/1/Asyraf%20%5BIIUMEJ%5D%202447_%20Editor%20Decision%20-%20Accept%201818.12.2022.pdf Yusof, Faridah (2022) The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region. iium engineering journal. (In Press)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic T Technology (General)
TP248.13 Biotechnology
spellingShingle T Technology (General)
TP248.13 Biotechnology
Yusof, Faridah
The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region
description The growth conditions of bacteria producing cold-active protease isolated from the Antarctic sample were screened using one-factor-at-time (OFAT). Then, crude protease of the strain was extracted during late logarithmic phase for enzymatic assay. A strain that showed the highest enzyme activity was selected for optimization via Response Surface Method (RSM). The parameters studied were incubation temperature (4 - 36 °C), pH media (4 – 10) and NaCl concentration (0 - 8 %). Based on the OFAT results, all eight strains showed the highest growth rate at 20 °C, pH 7 and 4% (w/v) NaCl. The assay showed that the crude enzyme extracted from strain SC8 exhibited significantly higher activity (0.20 U and 0.37 U) than the positive control (0.11 U and 0.31 U) at -20 °C and 20 °C. RSM suggested that the optimized setting for growth of SC8 were at 20.5 °C, pH 6.83 and 2.05% (w/v) of NaCl with the results of the bacterial growth rate value was 3.70 ± 0.06 x 106 cells/hr. Optimal growth conditions of SC8 from this study are useful for the large-scale production of cold-active protease in future.
format Article
author Yusof, Faridah
author_facet Yusof, Faridah
author_sort Yusof, Faridah
title The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region
title_short The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region
title_full The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region
title_fullStr The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region
title_full_unstemmed The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region
title_sort optimization of growth condition of the bacteria producing cold-active proteolytic enzyme from the antarctic region
publishDate 2022
url http://irep.iium.edu.my/102082/1/Asyraf%20%5BIIUMEJ%5D%202447_%20Editor%20Decision%20-%20Accept%201818.12.2022.pdf
http://irep.iium.edu.my/102082/
_version_ 1758577819587182592
score 13.211869

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