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Pathogenicity of different betanodavirus strains in Asian Seabass (Lates Calcarifer) under temperature fluctuation stress

Viral Nervous Necrosis (VNN) disease caused by betanodavirus infection is responsible for mass mortality in many marine cultured fish species. . Fish can acquire the disease by either horizontal or vertical transmission. Malaysian local betanodavirus strains were obtained from National Fish Health R...

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Main Authors: Khairul Asri, Muhammad Safwan, Abdullah, Azila, Nawi, Mohd Firdaus, Mohd Idris, Shaharah, Ramly, Rimatulhana, Mansor, Nur Nazifah
Format: Conference or Workshop Item
Language:English
Published: 2022
Subjects:
Q Science (General)
QR Microbiology
QR355 Virology
SH Aquaculture. Fisheries. Angling
SH151 Aquaculture - Fish Culture
Online Access:http://irep.iium.edu.my/100852/1/100852_Pathogenicity%20of%20different%20betanodavirus%20strains%20in%20Asian.pdf
http://irep.iium.edu.my/100852/
http://www.fhs-afs.net/pdf/pub/2.DAA11-HANDBOOK.pdf
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spelling my.iium.irep.1008522022-10-31T00:47:06Z http://irep.iium.edu.my/100852/ Pathogenicity of different betanodavirus strains in Asian Seabass (Lates Calcarifer) under temperature fluctuation stress Khairul Asri, Muhammad Safwan Abdullah, Azila Nawi, Mohd Firdaus Mohd Idris, Shaharah Ramly, Rimatulhana Mansor, Nur Nazifah Q Science (General) QR Microbiology QR355 Virology SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture Viral Nervous Necrosis (VNN) disease caused by betanodavirus infection is responsible for mass mortality in many marine cultured fish species. . Fish can acquire the disease by either horizontal or vertical transmission. Malaysian local betanodavirus strains were obtained from National Fish Health Research Centre, Penang and cultured in E-11 cell line. The isolates are Bawal Emas (BE), AVA and KL 22. Fifty-percent tissue culture infective dose (TCID50) calculations for all the isolates were conducted to evaluate the concentration of the virus. In this study, three hundred and sixty healthy seabass juveniles (5 ± 2g) with size two to three inches were obtained from Marine Fish Aquaculture Research Division, Terengganu was divided equally in eight tanks that represent four treatment groups with duplicates; Group 1 (AVA strains), Group 2 (BE strains), Group 3 (KL22 strains) and Group 4 (control). All the fish were observed daily for any clinical changes and mortality that occurred. Upon clinical signs appearing and mortality, the fish were slaughtered immediately and subjected to a polymerase chain reaction (PCR), cell culture and histology. The experiment was terminated after thirty days period. TCID 50 for all the tested strains are as follow; KL 22 = 104 TCID50/mL, BE = 107 TCID50/mL and AVA = 106 TCID50/mL. KL22 strain was found to have the highest TCID 50 followed by AVA and BE. Similarly, the highest mortality was also recorded in the group challenged with the KL22 strain which also has the highest TCLD 50. All the dead and sick fishes tested positive for PCR. However, the histology finding indicated no vacuolation found in the cell suggesting an acute infection has occurred. 2022-08-22 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/100852/1/100852_Pathogenicity%20of%20different%20betanodavirus%20strains%20in%20Asian.pdf Khairul Asri, Muhammad Safwan and Abdullah, Azila and Nawi, Mohd Firdaus and Mohd Idris, Shaharah and Ramly, Rimatulhana and Mansor, Nur Nazifah (2022) Pathogenicity of different betanodavirus strains in Asian Seabass (Lates Calcarifer) under temperature fluctuation stress. In: 11th Symposium on Diseases in Asian Aquaculture (DAA11), 23-26 August 2022, Kuching, Sarawak. (Unpublished) http://www.fhs-afs.net/pdf/pub/2.DAA11-HANDBOOK.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
QR Microbiology
QR355 Virology
SH Aquaculture. Fisheries. Angling
SH151 Aquaculture - Fish Culture
spellingShingle Q Science (General)
QR Microbiology
QR355 Virology
SH Aquaculture. Fisheries. Angling
SH151 Aquaculture - Fish Culture
Khairul Asri, Muhammad Safwan
Abdullah, Azila
Nawi, Mohd Firdaus
Mohd Idris, Shaharah
Ramly, Rimatulhana
Mansor, Nur Nazifah
Pathogenicity of different betanodavirus strains in Asian Seabass (Lates Calcarifer) under temperature fluctuation stress
description Viral Nervous Necrosis (VNN) disease caused by betanodavirus infection is responsible for mass mortality in many marine cultured fish species. . Fish can acquire the disease by either horizontal or vertical transmission. Malaysian local betanodavirus strains were obtained from National Fish Health Research Centre, Penang and cultured in E-11 cell line. The isolates are Bawal Emas (BE), AVA and KL 22. Fifty-percent tissue culture infective dose (TCID50) calculations for all the isolates were conducted to evaluate the concentration of the virus. In this study, three hundred and sixty healthy seabass juveniles (5 ± 2g) with size two to three inches were obtained from Marine Fish Aquaculture Research Division, Terengganu was divided equally in eight tanks that represent four treatment groups with duplicates; Group 1 (AVA strains), Group 2 (BE strains), Group 3 (KL22 strains) and Group 4 (control). All the fish were observed daily for any clinical changes and mortality that occurred. Upon clinical signs appearing and mortality, the fish were slaughtered immediately and subjected to a polymerase chain reaction (PCR), cell culture and histology. The experiment was terminated after thirty days period. TCID 50 for all the tested strains are as follow; KL 22 = 104 TCID50/mL, BE = 107 TCID50/mL and AVA = 106 TCID50/mL. KL22 strain was found to have the highest TCID 50 followed by AVA and BE. Similarly, the highest mortality was also recorded in the group challenged with the KL22 strain which also has the highest TCLD 50. All the dead and sick fishes tested positive for PCR. However, the histology finding indicated no vacuolation found in the cell suggesting an acute infection has occurred.
format Conference or Workshop Item
author Khairul Asri, Muhammad Safwan
Abdullah, Azila
Nawi, Mohd Firdaus
Mohd Idris, Shaharah
Ramly, Rimatulhana
Mansor, Nur Nazifah
author_facet Khairul Asri, Muhammad Safwan
Abdullah, Azila
Nawi, Mohd Firdaus
Mohd Idris, Shaharah
Ramly, Rimatulhana
Mansor, Nur Nazifah
author_sort Khairul Asri, Muhammad Safwan
title Pathogenicity of different betanodavirus strains in Asian Seabass (Lates Calcarifer) under temperature fluctuation stress
title_short Pathogenicity of different betanodavirus strains in Asian Seabass (Lates Calcarifer) under temperature fluctuation stress
title_full Pathogenicity of different betanodavirus strains in Asian Seabass (Lates Calcarifer) under temperature fluctuation stress
title_fullStr Pathogenicity of different betanodavirus strains in Asian Seabass (Lates Calcarifer) under temperature fluctuation stress
title_full_unstemmed Pathogenicity of different betanodavirus strains in Asian Seabass (Lates Calcarifer) under temperature fluctuation stress
title_sort pathogenicity of different betanodavirus strains in asian seabass (lates calcarifer) under temperature fluctuation stress
publishDate 2022
url http://irep.iium.edu.my/100852/1/100852_Pathogenicity%20of%20different%20betanodavirus%20strains%20in%20Asian.pdf
http://irep.iium.edu.my/100852/
http://www.fhs-afs.net/pdf/pub/2.DAA11-HANDBOOK.pdf
_version_ 1748180284664184832
score 13.211869

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