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Characterization of Cervus timorensis velvet antler and its effect on biofilm formation of Candida species

Oral biofilms comprise extracellular polysaccharides and polymicrobial microorganisms. The objectives of the study were to characterize the deer velvet antler (DVA) compounds and their effect on Candida species biofilm formation with the hypothesis that DVA inhibits the biofilm of Candida spp. Liqui...

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Main Authors: Mohd Badri, Puteri Elysa Alia, Rismayuddin, Nurul Alia Risma, Mohd Kenali, Norzaiti, Darnis, Deny Susanti, Arzmi, Mohd Hafiz
Format: Article
Language:English
Published: Oxford University Press 2022
Subjects:
QR Microbiology
RK Dentistry
Online Access:http://irep.iium.edu.my/100533/7/100533_Characterization%20of%20Cervus%20timorensis%20velvet%20antler.pdf
http://irep.iium.edu.my/100533/
https://academic.oup.com/mmy/article-abstract/60/9/myac073/6696968?redirectedFrom=fulltext
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spelling my.iium.irep.1005332022-10-12T01:22:36Z http://irep.iium.edu.my/100533/ Characterization of Cervus timorensis velvet antler and its effect on biofilm formation of Candida species Mohd Badri, Puteri Elysa Alia Rismayuddin, Nurul Alia Risma Mohd Kenali, Norzaiti Darnis, Deny Susanti Arzmi, Mohd Hafiz QR Microbiology RK Dentistry Oral biofilms comprise extracellular polysaccharides and polymicrobial microorganisms. The objectives of the study were to characterize the deer velvet antler (DVA) compounds and their effect on Candida species biofilm formation with the hypothesis that DVA inhibits the biofilm of Candida spp. Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry (LC-QTOF-MS) was conducted to characterize the DVA compounds. To study the effect of DVA on biofilm, Candida albicans ATCC MYA-4901 (ALT5), AIDS isolate (ALC2), oral cancer isolate (ALC3), C. dubliniensis ATCCMYA-2975,C.glabrataATCC90030,C.krusei14 243,C.lusitaniaeATCC34449,C.parapsilosisATCC22019,andC.tropicalisATCC13803 were inoculated with DVA in separate wells of a 96-well plate containing RPMI-1640 followed by 72 h incubation. A total of 45 compounds were detected in the DVA extract. C. lusitaniae exhibited a higher percentage of biofilm biomass reduction when treated with DVA extract (66.10% ± 5.33), followed by ALC3 (44.12% ± 6.24). However, C. glabrata, C. krusei, and C. parapsilosis showed no reduction in biofilm biomass after being treated with DVA extract. Most Candida strains also exhibited decreased total cell count when treated with DVA extract, except for ALC3 and C. krusei. ALT5 had the lowest total cell count (0.17 × 105 cells/ml) when cultured with DVA extract. In conclusion, DVA extract inhibits Candida spp. biofilm formation except for C. glabrata, C. krusei, and C. parapsilosis. Oxford University Press 2022-09-13 Article PeerReviewed application/pdf en http://irep.iium.edu.my/100533/7/100533_Characterization%20of%20Cervus%20timorensis%20velvet%20antler.pdf Mohd Badri, Puteri Elysa Alia and Rismayuddin, Nurul Alia Risma and Mohd Kenali, Norzaiti and Darnis, Deny Susanti and Arzmi, Mohd Hafiz (2022) Characterization of Cervus timorensis velvet antler and its effect on biofilm formation of Candida species. Medical Mycology, 60 (9). pp. 1-13. ISSN 1369-3786 E-ISSN 1460-2709 https://academic.oup.com/mmy/article-abstract/60/9/myac073/6696968?redirectedFrom=fulltext 10.1093/mmy/myac073
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QR Microbiology
RK Dentistry
spellingShingle QR Microbiology
RK Dentistry
Mohd Badri, Puteri Elysa Alia
Rismayuddin, Nurul Alia Risma
Mohd Kenali, Norzaiti
Darnis, Deny Susanti
Arzmi, Mohd Hafiz
Characterization of Cervus timorensis velvet antler and its effect on biofilm formation of Candida species
description Oral biofilms comprise extracellular polysaccharides and polymicrobial microorganisms. The objectives of the study were to characterize the deer velvet antler (DVA) compounds and their effect on Candida species biofilm formation with the hypothesis that DVA inhibits the biofilm of Candida spp. Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry (LC-QTOF-MS) was conducted to characterize the DVA compounds. To study the effect of DVA on biofilm, Candida albicans ATCC MYA-4901 (ALT5), AIDS isolate (ALC2), oral cancer isolate (ALC3), C. dubliniensis ATCCMYA-2975,C.glabrataATCC90030,C.krusei14 243,C.lusitaniaeATCC34449,C.parapsilosisATCC22019,andC.tropicalisATCC13803 were inoculated with DVA in separate wells of a 96-well plate containing RPMI-1640 followed by 72 h incubation. A total of 45 compounds were detected in the DVA extract. C. lusitaniae exhibited a higher percentage of biofilm biomass reduction when treated with DVA extract (66.10% ± 5.33), followed by ALC3 (44.12% ± 6.24). However, C. glabrata, C. krusei, and C. parapsilosis showed no reduction in biofilm biomass after being treated with DVA extract. Most Candida strains also exhibited decreased total cell count when treated with DVA extract, except for ALC3 and C. krusei. ALT5 had the lowest total cell count (0.17 × 105 cells/ml) when cultured with DVA extract. In conclusion, DVA extract inhibits Candida spp. biofilm formation except for C. glabrata, C. krusei, and C. parapsilosis.
format Article
author Mohd Badri, Puteri Elysa Alia
Rismayuddin, Nurul Alia Risma
Mohd Kenali, Norzaiti
Darnis, Deny Susanti
Arzmi, Mohd Hafiz
author_facet Mohd Badri, Puteri Elysa Alia
Rismayuddin, Nurul Alia Risma
Mohd Kenali, Norzaiti
Darnis, Deny Susanti
Arzmi, Mohd Hafiz
author_sort Mohd Badri, Puteri Elysa Alia
title Characterization of Cervus timorensis velvet antler and its effect on biofilm formation of Candida species
title_short Characterization of Cervus timorensis velvet antler and its effect on biofilm formation of Candida species
title_full Characterization of Cervus timorensis velvet antler and its effect on biofilm formation of Candida species
title_fullStr Characterization of Cervus timorensis velvet antler and its effect on biofilm formation of Candida species
title_full_unstemmed Characterization of Cervus timorensis velvet antler and its effect on biofilm formation of Candida species
title_sort characterization of cervus timorensis velvet antler and its effect on biofilm formation of candida species
publisher Oxford University Press
publishDate 2022
url http://irep.iium.edu.my/100533/7/100533_Characterization%20of%20Cervus%20timorensis%20velvet%20antler.pdf
http://irep.iium.edu.my/100533/
https://academic.oup.com/mmy/article-abstract/60/9/myac073/6696968?redirectedFrom=fulltext
_version_ 1748180259359948800
score 13.211869

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