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Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced model: a 96-hour behavioral study

Parkinson’s disease (PD) is the most common brain motor disorder, characterized by a substantial loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc). Motor impairments, such as dyskinesia, bradykinesia, and resting tremors, are the hallmarks of PD. Despite ongoing research, the...

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Main Authors: RazaliI, Khairiah, Mohd Nasir, Mohd Hamzah, Othman, Noratikah, Doolaanea, Abd Almonem, Kumar, Jaya, Ibrahim, Wisam Nabeel, Mohamed, Wael Mohamed Yousef
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Language:English
Published: Public Library of Science 2022
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R Medicine (General)
Online Access:http://irep.iium.edu.my/100428/7/100428_Characterization%20of%20neurobehavioral%20pattern%20in%20a%20zebrafish.pdf
http://irep.iium.edu.my/100428/
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0274844&type=printable
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spelling my.iium.irep.1004282022-10-05T07:31:21Z http://irep.iium.edu.my/100428/ Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced model: a 96-hour behavioral study RazaliI, Khairiah Mohd Nasir, Mohd Hamzah Othman, Noratikah Doolaanea, Abd Almonem Kumar, Jaya Ibrahim, Wisam Nabeel Mohamed, Wael Mohamed Yousef R Medicine (General) Parkinson’s disease (PD) is the most common brain motor disorder, characterized by a substantial loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc). Motor impairments, such as dyskinesia, bradykinesia, and resting tremors, are the hallmarks of PD. Despite ongoing research, the exact PD pathogenesis remains elusive due to the disease intricacy and difficulty in conducting human studies. Zebrafish (Danio rerio) has emerged as an ideal model for researching PD pathophysiology. Even though 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been used to induce PD in zebrafish, behavioural findings are frequently limited to a single time point (24 hours post-injection). In this sense, we aim to demonstrate the effects of MPTP on zebrafish swimming behaviour at multiple time points. We administered a single dosage of MPTP (200μg/g bw) via intraperitoneal injection (i/p) and assessed the locomotor activity and swimming pattern at 0h, 24h, and 96h post-injection through an open field test. Analysis of the behaviour revealed significant reductions in swimming velocity (cm/s) and distance travelled (cm), concurrent with an increase in freezing maintenance (duration and bouts) in zebrafish injected with MPTP. In addition, the MPTP-injected zebrafish exhibited complex swimming patterns, as measured by the turn angle, meander, and angular velocity, and showed abnormal swimming phenotypes, including freezing, looping, and erratic movement. To conclude, MPTP administration into adult zebrafish induced hypolocomotion and elicited motor incoordination. Plus, the effects of MPTP were observable 24 hours after the injection and still detectable 96 hours later. These findings contribute to the understanding of MPTP effects on adult zebrafish, particularly in terms of swimming behaviours, and may pave the way for a better understanding of the establishment of PD animal models in the future. Public Library of Science 2022-10-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/100428/7/100428_Characterization%20of%20neurobehavioral%20pattern%20in%20a%20zebrafish.pdf RazaliI, Khairiah and Mohd Nasir, Mohd Hamzah and Othman, Noratikah and Doolaanea, Abd Almonem and Kumar, Jaya and Ibrahim, Wisam Nabeel and Mohamed, Wael Mohamed Yousef (2022) Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced model: a 96-hour behavioral study. PLOS ONE. pp. 1-18. E-ISSN 1932-6203 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0274844&type=printable 10.1371/journal.pone.0274844
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
RazaliI, Khairiah
Mohd Nasir, Mohd Hamzah
Othman, Noratikah
Doolaanea, Abd Almonem
Kumar, Jaya
Ibrahim, Wisam Nabeel
Mohamed, Wael Mohamed Yousef
Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced model: a 96-hour behavioral study
description Parkinson’s disease (PD) is the most common brain motor disorder, characterized by a substantial loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc). Motor impairments, such as dyskinesia, bradykinesia, and resting tremors, are the hallmarks of PD. Despite ongoing research, the exact PD pathogenesis remains elusive due to the disease intricacy and difficulty in conducting human studies. Zebrafish (Danio rerio) has emerged as an ideal model for researching PD pathophysiology. Even though 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been used to induce PD in zebrafish, behavioural findings are frequently limited to a single time point (24 hours post-injection). In this sense, we aim to demonstrate the effects of MPTP on zebrafish swimming behaviour at multiple time points. We administered a single dosage of MPTP (200μg/g bw) via intraperitoneal injection (i/p) and assessed the locomotor activity and swimming pattern at 0h, 24h, and 96h post-injection through an open field test. Analysis of the behaviour revealed significant reductions in swimming velocity (cm/s) and distance travelled (cm), concurrent with an increase in freezing maintenance (duration and bouts) in zebrafish injected with MPTP. In addition, the MPTP-injected zebrafish exhibited complex swimming patterns, as measured by the turn angle, meander, and angular velocity, and showed abnormal swimming phenotypes, including freezing, looping, and erratic movement. To conclude, MPTP administration into adult zebrafish induced hypolocomotion and elicited motor incoordination. Plus, the effects of MPTP were observable 24 hours after the injection and still detectable 96 hours later. These findings contribute to the understanding of MPTP effects on adult zebrafish, particularly in terms of swimming behaviours, and may pave the way for a better understanding of the establishment of PD animal models in the future.
format Article
author RazaliI, Khairiah
Mohd Nasir, Mohd Hamzah
Othman, Noratikah
Doolaanea, Abd Almonem
Kumar, Jaya
Ibrahim, Wisam Nabeel
Mohamed, Wael Mohamed Yousef
author_facet RazaliI, Khairiah
Mohd Nasir, Mohd Hamzah
Othman, Noratikah
Doolaanea, Abd Almonem
Kumar, Jaya
Ibrahim, Wisam Nabeel
Mohamed, Wael Mohamed Yousef
author_sort RazaliI, Khairiah
title Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced model: a 96-hour behavioral study
title_short Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced model: a 96-hour behavioral study
title_full Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced model: a 96-hour behavioral study
title_fullStr Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced model: a 96-hour behavioral study
title_full_unstemmed Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced model: a 96-hour behavioral study
title_sort characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (mptp)-induced model: a 96-hour behavioral study
publisher Public Library of Science
publishDate 2022
url http://irep.iium.edu.my/100428/7/100428_Characterization%20of%20neurobehavioral%20pattern%20in%20a%20zebrafish.pdf
http://irep.iium.edu.my/100428/
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0274844&type=printable
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score 13.223943

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