Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia
Background Breast cancer is an ongoing issue that is plaguing many young women especially within the regions of Asia. Many factors have been studied thoroughly and have been ruled out over the years. Nevertheless, emergent studies towards the gut microbiome suggest its potential as another cause or...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| 格式: | Article |
| 語言: | English English |
| 出版: |
British Society of Gastroenterology and BMJ
2022
|
| 主題: | |
| 在線閱讀: | http://irep.iium.edu.my/100177/1/HFA%20IDDF2022_abs_eposter.pdf http://irep.iium.edu.my/100177/7/100177_Gut%20microbiome%20of%20women%20diagnosed.pdf http://irep.iium.edu.my/100177/ http://dx.doi.org/10.1136/gutjnl-2022-IDDF.244 |
| 標簽: |
添加標簽
沒有標簽, 成為第一個標記此記錄!
|
| id |
my.iium.irep.100177 |
|---|---|
| record_format |
dspace |
| spelling |
my.iium.irep.1001772022-09-26T02:22:43Z http://irep.iium.edu.my/100177/ Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia Abd Khalid, Nurul Syazwani Mahno, Noor Ezmas Dean Tay, Darren Ahmad, Hajar Fauzan Kasian, Junaini Othman, Ahmad Faidzal Alias, Norsyuhada Termizi, Sahrol Azmi Che Mohamed, Siti Kamariah QR Microbiology RD Surgery Background Breast cancer is an ongoing issue that is plaguing many young women especially within the regions of Asia. Many factors have been studied thoroughly and have been ruled out over the years. Nevertheless, emergent studies towards the gut microbiome suggest its potential as another cause or treatment to the current predicament. This study aims to elucidate the role of the microbiome in relation to breast cancer by comparing the observed gut flora within healthy individuals to those afflicted with breast cancer in Pahang, Malaysia. Methods Stool samples were collected from all 69 participants. 36 subjects were of newly diagnosed breast cancer patients, pre-treatment while 33 subjects were control group. Samples were homogenized with water prior to performing DNA extraction using standard protocols of QIAamp® PowerFecal® Pro Kit. Metagenome sequencing of V3 variable region of 16S rRNA gene was conducted on a NovaSEQ6000 (Illumina, San Diego, CA) using standard protocols. Data analysis was conducted on the MicrobiomeAnalyst platform using default settings. Results We report a total of 114 695 read counts generated which represents 704 operational taxonomic units (OTUs) from the raw data. IDDF2022-ABS-0263 Figure 1 shows the top 30 features observed among the groups at genus level where it was shown that Holdemanella and Akkermansia were more abundant within the cancer group. Significant beta-diversity results from IDDF2022-ABS-0263 Figure 2 (p-value ≤ 0.001) suggest that the composition between breast cancer patients and healthy individuals are distinct from each other. Furthermore, IDDF2022-ABS-0263 Figure 3A indicates that Holdemanella is significant to the study whereas IDDF2022-ABS-0263 Figure 3B shows that Akkermansia has positive correlations towards breast cancer patients.Conclusions This study found that the microbiome observed within breast cancer patients is indeed different from healthy individuals at least within Pahang, Malaysia. Genera of interest are Holdemanella and Akkermansia which are found to be more abundant within the cancer group. Further studies are warranted to better understand the roles of these features towards breast cancer development or treatment. British Society of Gastroenterology and BMJ 2022-09-24 Article PeerReviewed application/pdf en http://irep.iium.edu.my/100177/1/HFA%20IDDF2022_abs_eposter.pdf application/pdf en http://irep.iium.edu.my/100177/7/100177_Gut%20microbiome%20of%20women%20diagnosed.pdf Abd Khalid, Nurul Syazwani and Mahno, Noor Ezmas and Dean Tay, Darren and Ahmad, Hajar Fauzan and Kasian, Junaini and Othman, Ahmad Faidzal and Alias, Norsyuhada and Termizi, Sahrol Azmi and Che Mohamed, Siti Kamariah (2022) Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia. GUT, 71 (supplementary 2). A175-A177. ISSN 0017-5749 E-ISSN 1468-3288 http://dx.doi.org/10.1136/gutjnl-2022-IDDF.244 10.1136/gutjnl-2022-IDDF.244 |
| institution |
Universiti Islam Antarabangsa Malaysia |
| building |
IIUM Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
International Islamic University Malaysia |
| content_source |
IIUM Repository (IREP) |
| url_provider |
http://irep.iium.edu.my/ |
| language |
English English |
| topic |
QR Microbiology RD Surgery |
| spellingShingle |
QR Microbiology RD Surgery Abd Khalid, Nurul Syazwani Mahno, Noor Ezmas Dean Tay, Darren Ahmad, Hajar Fauzan Kasian, Junaini Othman, Ahmad Faidzal Alias, Norsyuhada Termizi, Sahrol Azmi Che Mohamed, Siti Kamariah Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia |
| description |
Background Breast cancer is an ongoing issue that is plaguing many young women especially within the regions of Asia. Many factors have been studied thoroughly and have been ruled out over the years. Nevertheless, emergent studies towards the gut microbiome suggest its potential as another cause or treatment to the current predicament. This study aims to elucidate the role of the microbiome in relation to breast cancer by comparing the observed gut flora within healthy individuals to those afflicted with breast cancer in Pahang, Malaysia.
Methods Stool samples were collected from all 69 participants. 36 subjects were of newly diagnosed breast cancer patients, pre-treatment while 33 subjects were control group. Samples were homogenized with water prior to performing DNA extraction using standard protocols of QIAamp® PowerFecal® Pro Kit. Metagenome sequencing of V3 variable region of 16S rRNA gene was conducted on a NovaSEQ6000 (Illumina, San Diego, CA) using standard protocols. Data analysis was conducted on the MicrobiomeAnalyst platform using default settings.
Results We report a total of 114 695 read counts generated which represents 704 operational taxonomic units (OTUs) from the raw data. IDDF2022-ABS-0263 Figure 1 shows the top 30 features observed among the groups at genus level where it was shown that Holdemanella and Akkermansia were more abundant within the cancer group. Significant beta-diversity results from IDDF2022-ABS-0263 Figure 2 (p-value ≤ 0.001) suggest that the composition between breast cancer patients and healthy individuals are distinct from each other. Furthermore, IDDF2022-ABS-0263 Figure 3A indicates that Holdemanella is significant to the study whereas IDDF2022-ABS-0263 Figure 3B shows that Akkermansia has positive correlations towards breast cancer patients.Conclusions This study found that the microbiome observed within breast cancer patients is indeed different from healthy individuals at least within Pahang, Malaysia. Genera of interest are Holdemanella and Akkermansia which are found to be more abundant within the cancer group. Further studies are warranted to better understand the roles of these features towards breast cancer development or treatment. |
| format |
Article |
| author |
Abd Khalid, Nurul Syazwani Mahno, Noor Ezmas Dean Tay, Darren Ahmad, Hajar Fauzan Kasian, Junaini Othman, Ahmad Faidzal Alias, Norsyuhada Termizi, Sahrol Azmi Che Mohamed, Siti Kamariah |
| author_facet |
Abd Khalid, Nurul Syazwani Mahno, Noor Ezmas Dean Tay, Darren Ahmad, Hajar Fauzan Kasian, Junaini Othman, Ahmad Faidzal Alias, Norsyuhada Termizi, Sahrol Azmi Che Mohamed, Siti Kamariah |
| author_sort |
Abd Khalid, Nurul Syazwani |
| title |
Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia |
| title_short |
Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia |
| title_full |
Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia |
| title_fullStr |
Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia |
| title_full_unstemmed |
Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia |
| title_sort |
gut microbiome of women diagnosed with breast cancer within pahang, malaysia |
| publisher |
British Society of Gastroenterology and BMJ |
| publishDate |
2022 |
| url |
http://irep.iium.edu.my/100177/1/HFA%20IDDF2022_abs_eposter.pdf http://irep.iium.edu.my/100177/7/100177_Gut%20microbiome%20of%20women%20diagnosed.pdf http://irep.iium.edu.my/100177/ http://dx.doi.org/10.1136/gutjnl-2022-IDDF.244 |
| _version_ |
1745562158838579200 |
| score |
13.251813 |