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Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia

Background Breast cancer is an ongoing issue that is plaguing many young women especially within the regions of Asia. Many factors have been studied thoroughly and have been ruled out over the years. Nevertheless, emergent studies towards the gut microbiome suggest its potential as another cause or...

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Main Authors: Abd Khalid, Nurul Syazwani, Mahno, Noor Ezmas, Dean Tay, Darren, Ahmad, Hajar Fauzan, Kasian, Junaini, Othman, Ahmad Faidzal, Alias, Norsyuhada, Termizi, Sahrol Azmi, Che Mohamed, Siti Kamariah
Format: Article
Language:English
English
Published: British Society of Gastroenterology and BMJ 2022
Subjects:
QR Microbiology
RD Surgery
Online Access:http://irep.iium.edu.my/100177/1/HFA%20IDDF2022_abs_eposter.pdf
http://irep.iium.edu.my/100177/7/100177_Gut%20microbiome%20of%20women%20diagnosed.pdf
http://irep.iium.edu.my/100177/
http://dx.doi.org/10.1136/gutjnl-2022-IDDF.244
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spelling my.iium.irep.1001772022-09-26T02:22:43Z http://irep.iium.edu.my/100177/ Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia Abd Khalid, Nurul Syazwani Mahno, Noor Ezmas Dean Tay, Darren Ahmad, Hajar Fauzan Kasian, Junaini Othman, Ahmad Faidzal Alias, Norsyuhada Termizi, Sahrol Azmi Che Mohamed, Siti Kamariah QR Microbiology RD Surgery Background Breast cancer is an ongoing issue that is plaguing many young women especially within the regions of Asia. Many factors have been studied thoroughly and have been ruled out over the years. Nevertheless, emergent studies towards the gut microbiome suggest its potential as another cause or treatment to the current predicament. This study aims to elucidate the role of the microbiome in relation to breast cancer by comparing the observed gut flora within healthy individuals to those afflicted with breast cancer in Pahang, Malaysia. Methods Stool samples were collected from all 69 participants. 36 subjects were of newly diagnosed breast cancer patients, pre-treatment while 33 subjects were control group. Samples were homogenized with water prior to performing DNA extraction using standard protocols of QIAamp® PowerFecal® Pro Kit. Metagenome sequencing of V3 variable region of 16S rRNA gene was conducted on a NovaSEQ6000 (Illumina, San Diego, CA) using standard protocols. Data analysis was conducted on the MicrobiomeAnalyst platform using default settings. Results We report a total of 114 695 read counts generated which represents 704 operational taxonomic units (OTUs) from the raw data. IDDF2022-ABS-0263 Figure 1 shows the top 30 features observed among the groups at genus level where it was shown that Holdemanella and Akkermansia were more abundant within the cancer group. Significant beta-diversity results from IDDF2022-ABS-0263 Figure 2 (p-value ≤ 0.001) suggest that the composition between breast cancer patients and healthy individuals are distinct from each other. Furthermore, IDDF2022-ABS-0263 Figure 3A indicates that Holdemanella is significant to the study whereas IDDF2022-ABS-0263 Figure 3B shows that Akkermansia has positive correlations towards breast cancer patients.Conclusions This study found that the microbiome observed within breast cancer patients is indeed different from healthy individuals at least within Pahang, Malaysia. Genera of interest are Holdemanella and Akkermansia which are found to be more abundant within the cancer group. Further studies are warranted to better understand the roles of these features towards breast cancer development or treatment. British Society of Gastroenterology and BMJ 2022-09-24 Article PeerReviewed application/pdf en http://irep.iium.edu.my/100177/1/HFA%20IDDF2022_abs_eposter.pdf application/pdf en http://irep.iium.edu.my/100177/7/100177_Gut%20microbiome%20of%20women%20diagnosed.pdf Abd Khalid, Nurul Syazwani and Mahno, Noor Ezmas and Dean Tay, Darren and Ahmad, Hajar Fauzan and Kasian, Junaini and Othman, Ahmad Faidzal and Alias, Norsyuhada and Termizi, Sahrol Azmi and Che Mohamed, Siti Kamariah (2022) Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia. GUT, 71 (supplementary 2). A175-A177. ISSN 0017-5749 E-ISSN 1468-3288 http://dx.doi.org/10.1136/gutjnl-2022-IDDF.244 10.1136/gutjnl-2022-IDDF.244
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QR Microbiology
RD Surgery
spellingShingle QR Microbiology
RD Surgery
Abd Khalid, Nurul Syazwani
Mahno, Noor Ezmas
Dean Tay, Darren
Ahmad, Hajar Fauzan
Kasian, Junaini
Othman, Ahmad Faidzal
Alias, Norsyuhada
Termizi, Sahrol Azmi
Che Mohamed, Siti Kamariah
Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia
description Background Breast cancer is an ongoing issue that is plaguing many young women especially within the regions of Asia. Many factors have been studied thoroughly and have been ruled out over the years. Nevertheless, emergent studies towards the gut microbiome suggest its potential as another cause or treatment to the current predicament. This study aims to elucidate the role of the microbiome in relation to breast cancer by comparing the observed gut flora within healthy individuals to those afflicted with breast cancer in Pahang, Malaysia. Methods Stool samples were collected from all 69 participants. 36 subjects were of newly diagnosed breast cancer patients, pre-treatment while 33 subjects were control group. Samples were homogenized with water prior to performing DNA extraction using standard protocols of QIAamp® PowerFecal® Pro Kit. Metagenome sequencing of V3 variable region of 16S rRNA gene was conducted on a NovaSEQ6000 (Illumina, San Diego, CA) using standard protocols. Data analysis was conducted on the MicrobiomeAnalyst platform using default settings. Results We report a total of 114 695 read counts generated which represents 704 operational taxonomic units (OTUs) from the raw data. IDDF2022-ABS-0263 Figure 1 shows the top 30 features observed among the groups at genus level where it was shown that Holdemanella and Akkermansia were more abundant within the cancer group. Significant beta-diversity results from IDDF2022-ABS-0263 Figure 2 (p-value ≤ 0.001) suggest that the composition between breast cancer patients and healthy individuals are distinct from each other. Furthermore, IDDF2022-ABS-0263 Figure 3A indicates that Holdemanella is significant to the study whereas IDDF2022-ABS-0263 Figure 3B shows that Akkermansia has positive correlations towards breast cancer patients.Conclusions This study found that the microbiome observed within breast cancer patients is indeed different from healthy individuals at least within Pahang, Malaysia. Genera of interest are Holdemanella and Akkermansia which are found to be more abundant within the cancer group. Further studies are warranted to better understand the roles of these features towards breast cancer development or treatment.
format Article
author Abd Khalid, Nurul Syazwani
Mahno, Noor Ezmas
Dean Tay, Darren
Ahmad, Hajar Fauzan
Kasian, Junaini
Othman, Ahmad Faidzal
Alias, Norsyuhada
Termizi, Sahrol Azmi
Che Mohamed, Siti Kamariah
author_facet Abd Khalid, Nurul Syazwani
Mahno, Noor Ezmas
Dean Tay, Darren
Ahmad, Hajar Fauzan
Kasian, Junaini
Othman, Ahmad Faidzal
Alias, Norsyuhada
Termizi, Sahrol Azmi
Che Mohamed, Siti Kamariah
author_sort Abd Khalid, Nurul Syazwani
title Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia
title_short Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia
title_full Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia
title_fullStr Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia
title_full_unstemmed Gut microbiome of women diagnosed with breast cancer within Pahang, Malaysia
title_sort gut microbiome of women diagnosed with breast cancer within pahang, malaysia
publisher British Society of Gastroenterology and BMJ
publishDate 2022
url http://irep.iium.edu.my/100177/1/HFA%20IDDF2022_abs_eposter.pdf
http://irep.iium.edu.my/100177/7/100177_Gut%20microbiome%20of%20women%20diagnosed.pdf
http://irep.iium.edu.my/100177/
http://dx.doi.org/10.1136/gutjnl-2022-IDDF.244
_version_ 1745562158838579200
score 13.211869

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