IMAGING SYSTEM FOR PAPER MICROFLUIDS

The background of the project is from a medical research where the detection of EBV(Epstein Barr Virus) delegates the early stages of possible cause for Nasopharyngeal Carcinoma. The technique used to detect the activity level of the virus in the human tissue is called Loop Mediated Isothermal Am...

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Bibliographic Details
Main Author: SANDHU, SANJEET SINGH
Format: Final Year Project
Language:English
Published: 2018
Online Access:http://utpedia.utp.edu.my/19204/1/FYP%202%20Sanjeet%20Final%20Report.pdf
http://utpedia.utp.edu.my/19204/
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Summary:The background of the project is from a medical research where the detection of EBV(Epstein Barr Virus) delegates the early stages of possible cause for Nasopharyngeal Carcinoma. The technique used to detect the activity level of the virus in the human tissue is called Loop Mediated Isothermal Amplification. The technique basically consists of heating up the tissue marked with dye and added with calcein which will then be heated to a temperature of 60 degree celcius. Under blue light, the sample will glow green in colour indicating the activity level of the virus. The brighter the intensity of the glow, the higher the activity of the virus in a particular sample. The project proposes to build a prototype that aims to reduce human involvement and run the tests autonomously to show the activity level of the virus. In this project, the sample is replaced with a green LED and the heating pad will be replaced with a thermoelectric peltier. The prototype is programmed to move and stop over the LED, and start reading the light intesity of the LED, only after the sample is at a certain temperature preferably 60 degrees celcius. The data read will then be saved and plotted in a graph and the pattern of the graph will be observed.