Cover Image

Isolation and characterisation of free-living nitrogen fixing bacteria from soil environment

Biological nitrogen fixation is a vital process in the nitrogen cycle which is performed by diazotrophs. Diazotrophs use nitrogenase enzymes to catalyse the conversion of dinitrogen gas into ammonia, which can be absorbed by plants. This present study aimed to isolate and characterise the free-livin...

Full description

Saved in:
Bibliographic Details
Main Author: Kong, Chia Huoi
Format: Final Year Project / Dissertation / Thesis
Published: 2024
Subjects:
Q Science (General)
QR Microbiology
QR180 Immunology
QR355 Virology
Online Access:http://eprints.utar.edu.my/6625/1/2104964_Dissertation.pdf
http://eprints.utar.edu.my/6625/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-utar-eprints.6625
record_format eprints
spelling my-utar-eprints.66252024-10-25T00:46:22Z Isolation and characterisation of free-living nitrogen fixing bacteria from soil environment Kong, Chia Huoi Q Science (General) QR Microbiology QR180 Immunology QR355 Virology Biological nitrogen fixation is a vital process in the nitrogen cycle which is performed by diazotrophs. Diazotrophs use nitrogenase enzymes to catalyse the conversion of dinitrogen gas into ammonia, which can be absorbed by plants. This present study aimed to isolate and characterise the free-living nitrogen fixing bacteria from the soil samples collected in Kampar. The locations involved were Zone F and Zone P in the UTAR Kampar campus as well as Eastlake areas. The nitrogen fixing bacteria from the collected soil samples were isolated using Burk’s medium. The bacterial isolates were characterised through Gram staining and the Gram-negative bacterial isolates were subjected to selected biochemical tests for the identification of Enterobacteriaceae. All the twenty bacterial isolates were screened for the nifH gene, but only three of them showed an approximate 360 bp amplicon after the PCR amplification using nifH primers. As such, the three bacterial isolates, N-6, E-I, and E-VI were selected for 16S rRNA sequencing, and the BLASTN results showed that they were identified as Bacillus luciferensis, Dickeya zeae, and Kosakonia oryzendophytica, respectively. However, the BLASTX results showed that the nifH gene was only present in E-I and E-VI. 2024-01 Final Year Project / Dissertation / Thesis NonPeerReviewed application/pdf http://eprints.utar.edu.my/6625/1/2104964_Dissertation.pdf Kong, Chia Huoi (2024) Isolation and characterisation of free-living nitrogen fixing bacteria from soil environment. Final Year Project, UTAR. http://eprints.utar.edu.my/6625/
institution Universiti Tunku Abdul Rahman
building UTAR Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tunku Abdul Rahman
content_source UTAR Institutional Repository
url_provider http://eprints.utar.edu.my
topic Q Science (General)
QR Microbiology
QR180 Immunology
QR355 Virology
spellingShingle Q Science (General)
QR Microbiology
QR180 Immunology
QR355 Virology
Kong, Chia Huoi
Isolation and characterisation of free-living nitrogen fixing bacteria from soil environment
description Biological nitrogen fixation is a vital process in the nitrogen cycle which is performed by diazotrophs. Diazotrophs use nitrogenase enzymes to catalyse the conversion of dinitrogen gas into ammonia, which can be absorbed by plants. This present study aimed to isolate and characterise the free-living nitrogen fixing bacteria from the soil samples collected in Kampar. The locations involved were Zone F and Zone P in the UTAR Kampar campus as well as Eastlake areas. The nitrogen fixing bacteria from the collected soil samples were isolated using Burk’s medium. The bacterial isolates were characterised through Gram staining and the Gram-negative bacterial isolates were subjected to selected biochemical tests for the identification of Enterobacteriaceae. All the twenty bacterial isolates were screened for the nifH gene, but only three of them showed an approximate 360 bp amplicon after the PCR amplification using nifH primers. As such, the three bacterial isolates, N-6, E-I, and E-VI were selected for 16S rRNA sequencing, and the BLASTN results showed that they were identified as Bacillus luciferensis, Dickeya zeae, and Kosakonia oryzendophytica, respectively. However, the BLASTX results showed that the nifH gene was only present in E-I and E-VI.
format Final Year Project / Dissertation / Thesis
author Kong, Chia Huoi
author_facet Kong, Chia Huoi
author_sort Kong, Chia Huoi
title Isolation and characterisation of free-living nitrogen fixing bacteria from soil environment
title_short Isolation and characterisation of free-living nitrogen fixing bacteria from soil environment
title_full Isolation and characterisation of free-living nitrogen fixing bacteria from soil environment
title_fullStr Isolation and characterisation of free-living nitrogen fixing bacteria from soil environment
title_full_unstemmed Isolation and characterisation of free-living nitrogen fixing bacteria from soil environment
title_sort isolation and characterisation of free-living nitrogen fixing bacteria from soil environment
publishDate 2024
url http://eprints.utar.edu.my/6625/1/2104964_Dissertation.pdf
http://eprints.utar.edu.my/6625/
_version_ 1814943243384651776
score 13.211869

Similar Items