Cover Image

Antioxidant activity and cytotoxic effect of frankincense essential oil

One of the most rapidly expanding fields of research is the investigation of natural products particularly medicinal plants as a potential source of anticancer drugs. Frankincense essential oil, from the Burseraceae family has been used during religious ceremonies as a ritual incense. The oil is als...

Full description

Saved in:
Bibliographic Details
Main Author: Koh, San Aseana
Format: Final Year Project / Dissertation / Thesis
Published: 2023
Subjects:
Q Science (General)
R Medicine (General)
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Online Access:http://eprints.utar.edu.my/5763/1/fyp_BM_2023_SAK.pdf
http://eprints.utar.edu.my/5763/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-utar-eprints.5763
record_format eprints
spelling my-utar-eprints.57632023-09-11T13:13:03Z Antioxidant activity and cytotoxic effect of frankincense essential oil Koh, San Aseana Q Science (General) R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica One of the most rapidly expanding fields of research is the investigation of natural products particularly medicinal plants as a potential source of anticancer drugs. Frankincense essential oil, from the Burseraceae family has been used during religious ceremonies as a ritual incense. The oil is also used to cure wounds, treatment of allergies, snake and bug bites, colds, coughs, diarrhoea, headaches, syphilis, stomach aches and backache. In this study, purchased frankincense essential oil was used and evaluated for its polarity using thin layer chromatography (TLC), detection of classes of secondary metabolites via phytochemical analysis, antioxidant activity using DPPH assay and cytotoxicity on Vero and A549 cell lines via MTT assay. Numerous spots and a broader range of retention factor values were observed in hexane : ethyl acetate (10:3) indicating the presence of polar and non-polar bioactive compounds. The results correlate with the presence of phenols, tannin, coumarin, flavonoids, saponin, alkaloids and glycosides. In DPPH assay, both frankincense essential oil (v/v) and ascorbic acid presented an EC50 value of of 4.73 ± 0.01% and 4.60 ± 0.02 mg/mL, respectively. In MTT assay, the frankincense essential oil did not exhibit cytotoxicity on Vero cells. The essential oil and doxorubicin showed higher cytotoxicity towards the A549 cells with IC50 values of 76.04 ± 0.09 μg/mL and 0.19 ± 0.10 μg/mL for 24- and 72-hours, respectively. This result iii indicates Frankincense essential oil could be a potential antioxidant agent with low toxicity to normal cells. Thus, further investigations should be conducted using pure isolates from the resin to further elucidate its capability as a pharmaceutical drug. 2023-01 Final Year Project / Dissertation / Thesis NonPeerReviewed application/pdf http://eprints.utar.edu.my/5763/1/fyp_BM_2023_SAK.pdf Koh, San Aseana (2023) Antioxidant activity and cytotoxic effect of frankincense essential oil. Final Year Project, UTAR. http://eprints.utar.edu.my/5763/
institution Universiti Tunku Abdul Rahman
building UTAR Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tunku Abdul Rahman
content_source UTAR Institutional Repository
url_provider http://eprints.utar.edu.my
topic Q Science (General)
R Medicine (General)
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
spellingShingle Q Science (General)
R Medicine (General)
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Koh, San Aseana
Antioxidant activity and cytotoxic effect of frankincense essential oil
description One of the most rapidly expanding fields of research is the investigation of natural products particularly medicinal plants as a potential source of anticancer drugs. Frankincense essential oil, from the Burseraceae family has been used during religious ceremonies as a ritual incense. The oil is also used to cure wounds, treatment of allergies, snake and bug bites, colds, coughs, diarrhoea, headaches, syphilis, stomach aches and backache. In this study, purchased frankincense essential oil was used and evaluated for its polarity using thin layer chromatography (TLC), detection of classes of secondary metabolites via phytochemical analysis, antioxidant activity using DPPH assay and cytotoxicity on Vero and A549 cell lines via MTT assay. Numerous spots and a broader range of retention factor values were observed in hexane : ethyl acetate (10:3) indicating the presence of polar and non-polar bioactive compounds. The results correlate with the presence of phenols, tannin, coumarin, flavonoids, saponin, alkaloids and glycosides. In DPPH assay, both frankincense essential oil (v/v) and ascorbic acid presented an EC50 value of of 4.73 ± 0.01% and 4.60 ± 0.02 mg/mL, respectively. In MTT assay, the frankincense essential oil did not exhibit cytotoxicity on Vero cells. The essential oil and doxorubicin showed higher cytotoxicity towards the A549 cells with IC50 values of 76.04 ± 0.09 μg/mL and 0.19 ± 0.10 μg/mL for 24- and 72-hours, respectively. This result iii indicates Frankincense essential oil could be a potential antioxidant agent with low toxicity to normal cells. Thus, further investigations should be conducted using pure isolates from the resin to further elucidate its capability as a pharmaceutical drug.
format Final Year Project / Dissertation / Thesis
author Koh, San Aseana
author_facet Koh, San Aseana
author_sort Koh, San Aseana
title Antioxidant activity and cytotoxic effect of frankincense essential oil
title_short Antioxidant activity and cytotoxic effect of frankincense essential oil
title_full Antioxidant activity and cytotoxic effect of frankincense essential oil
title_fullStr Antioxidant activity and cytotoxic effect of frankincense essential oil
title_full_unstemmed Antioxidant activity and cytotoxic effect of frankincense essential oil
title_sort antioxidant activity and cytotoxic effect of frankincense essential oil
publishDate 2023
url http://eprints.utar.edu.my/5763/1/fyp_BM_2023_SAK.pdf
http://eprints.utar.edu.my/5763/
_version_ 1778167135600640000
score 13.211869

Similar Items