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Influence of sugar and stevia to the cariogenicity of oral streptobacilli

Dental caries is one of the major health concerns caused by the acidogenic and cariogenic plaque bacteria which are frequently exposed to sugar. The colonization of rare oral streptobacilli could induce the occurrence of dental caries as they are biofilm-forming and acid producing oral bacteria. Thi...

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Main Author: Sai, Mei Ying
Format: Final Year Project / Dissertation / Thesis
Published: 2023
Subjects:
Q Science (General)
QH301 Biology
Online Access:http://eprints.utar.edu.my/5654/1/fyp_2023_MB_SMY.pdf
http://eprints.utar.edu.my/5654/
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spelling my-utar-eprints.56542023-09-11T14:07:38Z Influence of sugar and stevia to the cariogenicity of oral streptobacilli Sai, Mei Ying Q Science (General) QH301 Biology Dental caries is one of the major health concerns caused by the acidogenic and cariogenic plaque bacteria which are frequently exposed to sugar. The colonization of rare oral streptobacilli could induce the occurrence of dental caries as they are biofilm-forming and acid producing oral bacteria. This project aims to study three aspects that involve in the cariogenicity which are the biofilm-forming strength, acidogenicity, and thickness of biofilm formation. Hence, three different growth conditions, including sugar free condition, BHI broth supplemented with 1% (w/v) sugar (sugar-dependent, SD), and BHI broth supplemented with 0.15% (v/v) stevia (stevia-dependent, StD) were implemented. A total of three target isolates were isolated from the dental plaque in which two were Gram-positive, catalase-positive and one was Gram-negative, catalase negative. In the MTP assay, biofilm-forming strength of the isolates were assessed quantitatively based on the intensity of solubilized 0.1% (w/v) crystal violet stained in the biofilm. One target isolate showed significant outcome (p<0.05) of moderate biofilm-forming strength in SD condition and weak biofilm-forming strength in StD condition whereas another two isolates were excluded as they were unable to grow in broth medium and lead to pooroutcome in the MTP assay, respectively. The Gram-positive target isolate was identified as Bacillus sp. using 16S rRNA sequencing Besides, the scanning electron microscopic examination showed significant increased (p<0.05) in the thickness of biofilm formation of the Bacillus sp. under SD and StD conditions. Furthermore, the acidogenicity of the target isolate was determined via the acid detection test. The target isolate demonstrated a significant decreased (p<0.05) of pH in SD condition as compared to the StD condition. Overall, the outcomes demonstrated that Bacillus sp. may cause cariogenic effects under the presence of sugar. 2023-01 Final Year Project / Dissertation / Thesis NonPeerReviewed application/pdf http://eprints.utar.edu.my/5654/1/fyp_2023_MB_SMY.pdf Sai, Mei Ying (2023) Influence of sugar and stevia to the cariogenicity of oral streptobacilli. Final Year Project, UTAR. http://eprints.utar.edu.my/5654/
institution Universiti Tunku Abdul Rahman
building UTAR Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tunku Abdul Rahman
content_source UTAR Institutional Repository
url_provider http://eprints.utar.edu.my
topic Q Science (General)
QH301 Biology
spellingShingle Q Science (General)
QH301 Biology
Sai, Mei Ying
Influence of sugar and stevia to the cariogenicity of oral streptobacilli
description Dental caries is one of the major health concerns caused by the acidogenic and cariogenic plaque bacteria which are frequently exposed to sugar. The colonization of rare oral streptobacilli could induce the occurrence of dental caries as they are biofilm-forming and acid producing oral bacteria. This project aims to study three aspects that involve in the cariogenicity which are the biofilm-forming strength, acidogenicity, and thickness of biofilm formation. Hence, three different growth conditions, including sugar free condition, BHI broth supplemented with 1% (w/v) sugar (sugar-dependent, SD), and BHI broth supplemented with 0.15% (v/v) stevia (stevia-dependent, StD) were implemented. A total of three target isolates were isolated from the dental plaque in which two were Gram-positive, catalase-positive and one was Gram-negative, catalase negative. In the MTP assay, biofilm-forming strength of the isolates were assessed quantitatively based on the intensity of solubilized 0.1% (w/v) crystal violet stained in the biofilm. One target isolate showed significant outcome (p<0.05) of moderate biofilm-forming strength in SD condition and weak biofilm-forming strength in StD condition whereas another two isolates were excluded as they were unable to grow in broth medium and lead to pooroutcome in the MTP assay, respectively. The Gram-positive target isolate was identified as Bacillus sp. using 16S rRNA sequencing Besides, the scanning electron microscopic examination showed significant increased (p<0.05) in the thickness of biofilm formation of the Bacillus sp. under SD and StD conditions. Furthermore, the acidogenicity of the target isolate was determined via the acid detection test. The target isolate demonstrated a significant decreased (p<0.05) of pH in SD condition as compared to the StD condition. Overall, the outcomes demonstrated that Bacillus sp. may cause cariogenic effects under the presence of sugar.
format Final Year Project / Dissertation / Thesis
author Sai, Mei Ying
author_facet Sai, Mei Ying
author_sort Sai, Mei Ying
title Influence of sugar and stevia to the cariogenicity of oral streptobacilli
title_short Influence of sugar and stevia to the cariogenicity of oral streptobacilli
title_full Influence of sugar and stevia to the cariogenicity of oral streptobacilli
title_fullStr Influence of sugar and stevia to the cariogenicity of oral streptobacilli
title_full_unstemmed Influence of sugar and stevia to the cariogenicity of oral streptobacilli
title_sort influence of sugar and stevia to the cariogenicity of oral streptobacilli
publishDate 2023
url http://eprints.utar.edu.my/5654/1/fyp_2023_MB_SMY.pdf
http://eprints.utar.edu.my/5654/
_version_ 1778167132633169920
score 13.211869

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