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ISSR markers assisted genetic diversity analysis Dioscorea hispida and selection of best variety for sustainable production

Plant breeding is a way of selection of a particular individual for the production of progeny by separating or combining desired characteristics. The objective of this study is to justify different characteristics of Dioscorea hispida (Ubi gadong) varieties and to select the best variety using molec...

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Main Authors:	Md Amirul, A., Nadiawati, Alias, Sarwar, Jahan, Abd Jamil, Z.
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	H Social Sciences (General) HD28 Management. Industrial Management
Online Access:	http://eprints.unisza.edu.my/769/1/FH03-FBIM-17-09116.pdf http://eprints.unisza.edu.my/769/
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id	my-unisza-ir.769
record_format	eprints
spelling	my-unisza-ir.7692020-10-27T03:58:43Z http://eprints.unisza.edu.my/769/ ISSR markers assisted genetic diversity analysis Dioscorea hispida and selection of best variety for sustainable production Md Amirul, A. Nadiawati, Alias Sarwar, Jahan Abd Jamil, Z. H Social Sciences (General) HD28 Management. Industrial Management Plant breeding is a way of selection of a particular individual for the production of progeny by separating or combining desired characteristics. The objective of this study is to justify different characteristics of Dioscorea hispida (Ubi gadong) varieties and to select the best variety using molecular techniques for sustainable commercial production at farmer's level. A total o( 160 germplasms of Ubi gadong were collected from different locations at the Terengganu and Kelantan states of Malaysia. Forty eight (48) out of 160 germplasms were selected as 'primary' selection phenotypically based on yield and other qualitative characters. Selected collections were then grown and maintained for ISSR markers assisted genetic diversity analysis. Overall plant growth and yield of tubers were also determined. A total of 12 ISSR markers were tested to justify the characteristics of Ubi gadong varieties among which 3 markers showed polymorphic bands and on an average 57.3% polymorphism were observed representing highest variation among those germplasms. The JSSR marker based on UPGMA cluster analysis grouped an 48 D. hispida into 10 vital groups that proved a vast genetic variation among germplasm collections. Therefore, hybridization should be made between hvo distant populations. The D. hispida is already 1>roved as the highest starch content tuber crops and very rich in vitamins with both micro and macro minerals. Considering all these criteria and results from marker assisted diversity analysis accessions that are far apart based on their genetic coefficient (like DH27 and DH71; DH30 and DH70; DH43 and DH62; DH45 and DH61; DH77 and DH61;DH78 and DH57) could be selected as parents for further breeding programs. This will bring about greater diversity which will lead to high productive index in terms of increase in yield and overall quality and for the ultimate target of sustainable Ubi gadong production. 2016 Conference or Workshop Item NonPeerReviewed text en http://eprints.unisza.edu.my/769/1/FH03-FBIM-17-09116.pdf Md Amirul, A. and Nadiawati, Alias and Sarwar, Jahan and Abd Jamil, Z. (2016) ISSR markers assisted genetic diversity analysis Dioscorea hispida and selection of best variety for sustainable production. In: Seminar Sustainable Dioscorea hispida Yam Production in Terengganu, Malaysia, 29-30 March 2016.
institution	Universiti Sultan Zainal Abidin
building	UNISZA Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sultan Zainal Abidin
content_source	UNISZA Institutional Repository
url_provider	https://eprints.unisza.edu.my/
language	English
topic	H Social Sciences (General) HD28 Management. Industrial Management
spellingShingle	H Social Sciences (General) HD28 Management. Industrial Management Md Amirul, A. Nadiawati, Alias Sarwar, Jahan Abd Jamil, Z. ISSR markers assisted genetic diversity analysis Dioscorea hispida and selection of best variety for sustainable production
description	Plant breeding is a way of selection of a particular individual for the production of progeny by separating or combining desired characteristics. The objective of this study is to justify different characteristics of Dioscorea hispida (Ubi gadong) varieties and to select the best variety using molecular techniques for sustainable commercial production at farmer's level. A total o( 160 germplasms of Ubi gadong were collected from different locations at the Terengganu and Kelantan states of Malaysia. Forty eight (48) out of 160 germplasms were selected as 'primary' selection phenotypically based on yield and other qualitative characters. Selected collections were then grown and maintained for ISSR markers assisted genetic diversity analysis. Overall plant growth and yield of tubers were also determined. A total of 12 ISSR markers were tested to justify the characteristics of Ubi gadong varieties among which 3 markers showed polymorphic bands and on an average 57.3% polymorphism were observed representing highest variation among those germplasms. The JSSR marker based on UPGMA cluster analysis grouped an 48 D. hispida into 10 vital groups that proved a vast genetic variation among germplasm collections. Therefore, hybridization should be made between hvo distant populations. The D. hispida is already 1>roved as the highest starch content tuber crops and very rich in vitamins with both micro and macro minerals. Considering all these criteria and results from marker assisted diversity analysis accessions that are far apart based on their genetic coefficient (like DH27 and DH71; DH30 and DH70; DH43 and DH62; DH45 and DH61; DH77 and DH61;DH78 and DH57) could be selected as parents for further breeding programs. This will bring about greater diversity which will lead to high productive index in terms of increase in yield and overall quality and for the ultimate target of sustainable Ubi gadong production.
format	Conference or Workshop Item
author	Md Amirul, A. Nadiawati, Alias Sarwar, Jahan Abd Jamil, Z.
author_facet	Md Amirul, A. Nadiawati, Alias Sarwar, Jahan Abd Jamil, Z.
author_sort	Md Amirul, A.
title	ISSR markers assisted genetic diversity analysis Dioscorea hispida and selection of best variety for sustainable production
title_short	ISSR markers assisted genetic diversity analysis Dioscorea hispida and selection of best variety for sustainable production
title_full	ISSR markers assisted genetic diversity analysis Dioscorea hispida and selection of best variety for sustainable production
title_fullStr	ISSR markers assisted genetic diversity analysis Dioscorea hispida and selection of best variety for sustainable production
title_full_unstemmed	ISSR markers assisted genetic diversity analysis Dioscorea hispida and selection of best variety for sustainable production
title_sort	issr markers assisted genetic diversity analysis dioscorea hispida and selection of best variety for sustainable production
publishDate	2016
url	http://eprints.unisza.edu.my/769/1/FH03-FBIM-17-09116.pdf http://eprints.unisza.edu.my/769/
_version_	1683234927300050944
score	13.211869

ISSR markers assisted genetic diversity analysis Dioscorea hispida and selection of best variety for sustainable production

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