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Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity

Background and aims: Peroxisome proliferator-activated receptor Ɣ2 (PPARƔ2) gene Pro12Ala polymorphism has been extensively studied in relation to obesity and its associated metabolic complications but the results were inconclusive. This study aimed to identify the genotypic and allelic frequencies...

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Main Authors: Mohd Khairi, Zahri@Johari, Rohayu Izanwati, Mohd Rawi, Nur Emiliana, Roslan
Format: Article
Language:English
Published: 2016
Subjects:
QH301 Biology
Online Access:http://eprints.unisza.edu.my/7583/1/FH02-FSK-16-06890.jpg
http://eprints.unisza.edu.my/7583/
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spelling my-unisza-ir.75832022-09-13T04:40:25Z http://eprints.unisza.edu.my/7583/ Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity Mohd Khairi, Zahri@Johari Rohayu Izanwati, Mohd Rawi Nur Emiliana, Roslan QH301 Biology Background and aims: Peroxisome proliferator-activated receptor Ɣ2 (PPARƔ2) gene Pro12Ala polymorphism has been extensively studied in relation to obesity and its associated metabolic complications but the results were inconclusive. This study aimed to identify the genotypic and allelic frequencies of PPARƔ2 gene and its association with anthropometric measurements, lipid profiles and the susceptibility for obesity in Malay subjects. Methods and results: This cross-sectional, comparative study involved 217 subjects (94 obese and 123 non-obese as controls). Anthropometric and lipid profiles were measured. Genotyping was performed by allele-specific PCR. Comparisons were made between the genotypes and the association of PPARƔ2 Pro12Ala polymorphism with obesity was evaluated. The Pro12Pro, Pro12Ala and Ala12Ala genotypic frequencies were significantly different between groups (88.3%/11.7%/0.0% vs. 97.6%/2.4%/0.0% respectively, P = 0.006).The Ala12 allele was more frequent in obese than the non-obese (5.9% vs. 1.1%, P = 0.007). Ala12 carriers were associated with higher BMI (P = 0.016), BF% (P = 0.019) and a trend towards higher BAI (P = 0.055) than the non-carriers. Besides age, high level of triglycerides and LDL-cholesterol, Ala12 allele (Adjusted OR = 5.46, 95% CI = 1.27–23.40; P = 0.022) were regarded as independent risk factors for obesity. Conclusions: This study indicates that the PPARƔ2 gene Pro12Ala polymorphism predisposes to obesity in Malay subjects and Ala12 allele could predict alterations in adipocyte and lipid metabolism among them. Age, triglycerides, LDL-cholesterol and Ala12 allele conferred to increased risk for obesity in this ethnicity. 2016-12 Article PeerReviewed image en http://eprints.unisza.edu.my/7583/1/FH02-FSK-16-06890.jpg Mohd Khairi, Zahri@Johari and Rohayu Izanwati, Mohd Rawi and Nur Emiliana, Roslan (2016) Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity. Meta Gene, 10. pp. 39-44. ISSN 22145400
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Mohd Khairi, Zahri@Johari
Rohayu Izanwati, Mohd Rawi
Nur Emiliana, Roslan
Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity
description Background and aims: Peroxisome proliferator-activated receptor Ɣ2 (PPARƔ2) gene Pro12Ala polymorphism has been extensively studied in relation to obesity and its associated metabolic complications but the results were inconclusive. This study aimed to identify the genotypic and allelic frequencies of PPARƔ2 gene and its association with anthropometric measurements, lipid profiles and the susceptibility for obesity in Malay subjects. Methods and results: This cross-sectional, comparative study involved 217 subjects (94 obese and 123 non-obese as controls). Anthropometric and lipid profiles were measured. Genotyping was performed by allele-specific PCR. Comparisons were made between the genotypes and the association of PPARƔ2 Pro12Ala polymorphism with obesity was evaluated. The Pro12Pro, Pro12Ala and Ala12Ala genotypic frequencies were significantly different between groups (88.3%/11.7%/0.0% vs. 97.6%/2.4%/0.0% respectively, P = 0.006).The Ala12 allele was more frequent in obese than the non-obese (5.9% vs. 1.1%, P = 0.007). Ala12 carriers were associated with higher BMI (P = 0.016), BF% (P = 0.019) and a trend towards higher BAI (P = 0.055) than the non-carriers. Besides age, high level of triglycerides and LDL-cholesterol, Ala12 allele (Adjusted OR = 5.46, 95% CI = 1.27–23.40; P = 0.022) were regarded as independent risk factors for obesity. Conclusions: This study indicates that the PPARƔ2 gene Pro12Ala polymorphism predisposes to obesity in Malay subjects and Ala12 allele could predict alterations in adipocyte and lipid metabolism among them. Age, triglycerides, LDL-cholesterol and Ala12 allele conferred to increased risk for obesity in this ethnicity.
format Article
author Mohd Khairi, Zahri@Johari
Rohayu Izanwati, Mohd Rawi
Nur Emiliana, Roslan
author_facet Mohd Khairi, Zahri@Johari
Rohayu Izanwati, Mohd Rawi
Nur Emiliana, Roslan
author_sort Mohd Khairi, Zahri@Johari
title Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity
title_short Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity
title_full Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity
title_fullStr Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity
title_full_unstemmed Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity
title_sort contribution of the pro12ala polymorphism of peroxisome proliferator-activated receptor ɣ2 gene in relation to obesity
publishDate 2016
url http://eprints.unisza.edu.my/7583/1/FH02-FSK-16-06890.jpg
http://eprints.unisza.edu.my/7583/
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score 13.211869

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