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Heterologous expression of the Streptococcus pneumoniae yoeB and pezT toxin genes is lethal in Chlorella vulgaris

Chlorella vulgaris is a eukaryotic microalga with potential for the production of biofuels. However, its thick and rigid cell wall is an impediment to cost-effective, large-scale harvesting of biofuels from these cells. Bacterial toxin-antitoxin (TA) systems, comprising of a stable proteic toxin and...

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Main Authors: Yeo, Chew Chieng, Ng, Shet Lee, Jennifer Ann, Harikrishna
Format: Article
Language:English
English
Published: Elsevier B.V. 2016
Subjects:
QH301 Biology
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spelling my-unisza-ir.75272022-09-13T05:04:46Z http://eprints.unisza.edu.my/7527/ Heterologous expression of the Streptococcus pneumoniae yoeB and pezT toxin genes is lethal in Chlorella vulgaris Yeo, Chew Chieng Ng, Shet Lee Jennifer Ann, Harikrishna QH301 Biology Chlorella vulgaris is a eukaryotic microalga with potential for the production of biofuels. However, its thick and rigid cell wall is an impediment to cost-effective, large-scale harvesting of biofuels from these cells. Bacterial toxin-antitoxin (TA) systems, comprising of a stable proteic toxin and its labile cognate antitoxin, have no known homologs in eukaryotic cells. Several bacterial TA toxins have been found to be lethal when expressed in eukaryotes such as yeasts, animal and human cell lines. In this study, the functionality of the yoeBSpn and pezT toxin genes from the Gram-positive bacterium Streptococcus pneumoniae in C. vulgaris was investigated using a two-component inducible expression system. The yoeBSpn and pezT toxin genes were each cloned as green fluorescent protein (GFP) fusion constructs and introduced into C. vulgaris by Agrobacterium tumefaciens-mediated co-transformation with recombinant activator and responder vectors. Following induction for the expression of the toxin-GFP fusion transgenes, GFP fluorescence was observed in the transformed C. vulgaris cells which also showed signs of cellular damage and lysis. This is the first report of the lethal expression of bacterial TA toxins in eukaryotic microalgae, which can form the basis of a novel method for harvesting of microalgal cellular contents. Elsevier B.V. 2016-11 Article PeerReviewed image en http://eprints.unisza.edu.my/7527/1/FH02-FP-16-06304.jpg image en http://eprints.unisza.edu.my/7527/2/FH02-FP-17-08124.jpg Yeo, Chew Chieng and Ng, Shet Lee and Jennifer Ann, Harikrishna (2016) Heterologous expression of the Streptococcus pneumoniae yoeB and pezT toxin genes is lethal in Chlorella vulgaris. Algal Research, 19. pp. 21-29. ISSN 22119264
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
English
topic QH301 Biology
spellingShingle QH301 Biology
Yeo, Chew Chieng
Ng, Shet Lee
Jennifer Ann, Harikrishna
Heterologous expression of the Streptococcus pneumoniae yoeB and pezT toxin genes is lethal in Chlorella vulgaris
description Chlorella vulgaris is a eukaryotic microalga with potential for the production of biofuels. However, its thick and rigid cell wall is an impediment to cost-effective, large-scale harvesting of biofuels from these cells. Bacterial toxin-antitoxin (TA) systems, comprising of a stable proteic toxin and its labile cognate antitoxin, have no known homologs in eukaryotic cells. Several bacterial TA toxins have been found to be lethal when expressed in eukaryotes such as yeasts, animal and human cell lines. In this study, the functionality of the yoeBSpn and pezT toxin genes from the Gram-positive bacterium Streptococcus pneumoniae in C. vulgaris was investigated using a two-component inducible expression system. The yoeBSpn and pezT toxin genes were each cloned as green fluorescent protein (GFP) fusion constructs and introduced into C. vulgaris by Agrobacterium tumefaciens-mediated co-transformation with recombinant activator and responder vectors. Following induction for the expression of the toxin-GFP fusion transgenes, GFP fluorescence was observed in the transformed C. vulgaris cells which also showed signs of cellular damage and lysis. This is the first report of the lethal expression of bacterial TA toxins in eukaryotic microalgae, which can form the basis of a novel method for harvesting of microalgal cellular contents.
format Article
author Yeo, Chew Chieng
Ng, Shet Lee
Jennifer Ann, Harikrishna
author_facet Yeo, Chew Chieng
Ng, Shet Lee
Jennifer Ann, Harikrishna
author_sort Yeo, Chew Chieng
title Heterologous expression of the Streptococcus pneumoniae yoeB and pezT toxin genes is lethal in Chlorella vulgaris
title_short Heterologous expression of the Streptococcus pneumoniae yoeB and pezT toxin genes is lethal in Chlorella vulgaris
title_full Heterologous expression of the Streptococcus pneumoniae yoeB and pezT toxin genes is lethal in Chlorella vulgaris
title_fullStr Heterologous expression of the Streptococcus pneumoniae yoeB and pezT toxin genes is lethal in Chlorella vulgaris
title_full_unstemmed Heterologous expression of the Streptococcus pneumoniae yoeB and pezT toxin genes is lethal in Chlorella vulgaris
title_sort heterologous expression of the streptococcus pneumoniae yoeb and pezt toxin genes is lethal in chlorella vulgaris
publisher Elsevier B.V.
publishDate 2016
url http://eprints.unisza.edu.my/7527/1/FH02-FP-16-06304.jpg
http://eprints.unisza.edu.my/7527/2/FH02-FP-17-08124.jpg
http://eprints.unisza.edu.my/7527/
_version_ 1744358604381618176
score 13.211869

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