Staff View: Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)

Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)

E. longifolia is attracting interest due to its pharmacological properties and pro-vitality effects. In this study, an online SPE-LC approach using polystyrene divinyl benzene (PSDVB) and C18 columns was developed in obtaining chromatographic fingerprints of E. longifolia. E. longifolia root samples...

Full description

Saved in:

Bibliographic Details
Main Authors:	Hafizan, Juahir, Nor Nasriah, Zaini, Rozita, Osman
Format:	Article
Language:	English English English
Published:	MDPI AG 2016
Subjects:	QD Chemistry R Medicine (General)
Online Access:	http://eprints.unisza.edu.my/7368/1/FH02-ESERI-16-05986.pdf http://eprints.unisza.edu.my/7368/2/FH02-ESERI-16-06112.jpg http://eprints.unisza.edu.my/7368/3/FH02-ESERI-16-06936.jpg http://eprints.unisza.edu.my/7368/
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unisza-ir.7368
record_format	eprints
spelling	my-unisza-ir.73682022-09-13T04:43:40Z http://eprints.unisza.edu.my/7368/ Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC) Hafizan, Juahir Nor Nasriah, Zaini Rozita, Osman QD Chemistry R Medicine (General) E. longifolia is attracting interest due to its pharmacological properties and pro-vitality effects. In this study, an online SPE-LC approach using polystyrene divinyl benzene (PSDVB) and C18 columns was developed in obtaining chromatographic fingerprints of E. longifolia. E. longifolia root samples were extracted using pressurized liquid extraction (PLE) technique prior to online SPE-LC. The effects of mobile phase compositions and column switching time on the chromatographic fingerprint were optimized. Validation of the developed method was studied based on eurycomanone. Linearity was in the range of 5 to 50 µg¨mL´1 (r2 = 0.997) with 3.2% relative standard deviation of peak area. The developed method was used to analyze 14 E. longifolia root samples and 10 products (capsules). Selected chemometric techniques: cluster analysis (CA), discriminant analysis (DA), and principal component analysis (PCA) were applied to the fingerprint datasets of 37 selected peaks to evaluate the ability of the chromatographic fingerprint in classifying quality of E. longifolia. Three groups were obtained using CA. DA yielded 100% correlation coefficient with 19 discriminant compounds. Using PCA, E. longifolia root samples were clearly discriminated from the products. This study showed that the developed online SPE-LC method was able to provide comprehensive evaluation of E. longifolia samples for quality control purposes. MDPI AG 2016-05 Article PeerReviewed text en http://eprints.unisza.edu.my/7368/1/FH02-ESERI-16-05986.pdf image en http://eprints.unisza.edu.my/7368/2/FH02-ESERI-16-06112.jpg image en http://eprints.unisza.edu.my/7368/3/FH02-ESERI-16-06936.jpg Hafizan, Juahir and Nor Nasriah, Zaini and Rozita, Osman (2016) Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC). Molecules, 21 (5). pp. 1-10. ISSN 1420-3049
institution	Universiti Sultan Zainal Abidin
building	UNISZA Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sultan Zainal Abidin
content_source	UNISZA Institutional Repository
url_provider	https://eprints.unisza.edu.my/
language	English English English
topic	QD Chemistry R Medicine (General)
spellingShingle	QD Chemistry R Medicine (General) Hafizan, Juahir Nor Nasriah, Zaini Rozita, Osman Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)
description	E. longifolia is attracting interest due to its pharmacological properties and pro-vitality effects. In this study, an online SPE-LC approach using polystyrene divinyl benzene (PSDVB) and C18 columns was developed in obtaining chromatographic fingerprints of E. longifolia. E. longifolia root samples were extracted using pressurized liquid extraction (PLE) technique prior to online SPE-LC. The effects of mobile phase compositions and column switching time on the chromatographic fingerprint were optimized. Validation of the developed method was studied based on eurycomanone. Linearity was in the range of 5 to 50 µg¨mL´1 (r2 = 0.997) with 3.2% relative standard deviation of peak area. The developed method was used to analyze 14 E. longifolia root samples and 10 products (capsules). Selected chemometric techniques: cluster analysis (CA), discriminant analysis (DA), and principal component analysis (PCA) were applied to the fingerprint datasets of 37 selected peaks to evaluate the ability of the chromatographic fingerprint in classifying quality of E. longifolia. Three groups were obtained using CA. DA yielded 100% correlation coefficient with 19 discriminant compounds. Using PCA, E. longifolia root samples were clearly discriminated from the products. This study showed that the developed online SPE-LC method was able to provide comprehensive evaluation of E. longifolia samples for quality control purposes.
format	Article
author	Hafizan, Juahir Nor Nasriah, Zaini Rozita, Osman
author_facet	Hafizan, Juahir Nor Nasriah, Zaini Rozita, Osman
author_sort	Hafizan, Juahir
title	Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)
title_short	Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)
title_full	Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)
title_fullStr	Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)
title_full_unstemmed	Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)
title_sort	development of chromatographic fingerprints of eurycoma longifolia (tongkat ali) roots using online solid phase extraction-liquid chromatography (spe-lc)
publisher	MDPI AG
publishDate	2016
url	http://eprints.unisza.edu.my/7368/1/FH02-ESERI-16-05986.pdf http://eprints.unisza.edu.my/7368/2/FH02-ESERI-16-06112.jpg http://eprints.unisza.edu.my/7368/3/FH02-ESERI-16-06936.jpg http://eprints.unisza.edu.my/7368/
_version_	1744358599110426624
score	13.211869

Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)

Similar Items