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Light-harvesting complexes in photosystem II regulate glutathione-induced sensitivity of Arabidopsis guard cells to abscisic acid

Light-harvesting complexes (LHCs) in photosystem II (PSII) regulate glutathione (GSH) functions in plants. To investigate whether LHCs control GSH biosynthesis that modifies guard cell abscisic acid (ABA) sensitivity, we evaluated GSH content, stomatal aperture, reactive oxygen species (ROS), weight...

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Main Authors: Md. Sarwar, Jahan, Nashriyah, Mat, Mohd Nozulaidi, Nordin
Format: Article
Language:English
English
Published: Elsevier GmbH 2016
Subjects:
GE Environmental Sciences
Online Access:http://eprints.unisza.edu.my/7198/1/FH02-FBIM-16-05536.jpg
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spelling my-unisza-ir.71982022-09-13T05:13:56Z http://eprints.unisza.edu.my/7198/ Light-harvesting complexes in photosystem II regulate glutathione-induced sensitivity of Arabidopsis guard cells to abscisic acid Md. Sarwar, Jahan Nashriyah, Mat Mohd Nozulaidi, Nordin GE Environmental Sciences Light-harvesting complexes (LHCs) in photosystem II (PSII) regulate glutathione (GSH) functions in plants. To investigate whether LHCs control GSH biosynthesis that modifies guard cell abscisic acid (ABA) sensitivity, we evaluated GSH content, stomatal aperture, reactive oxygen species (ROS), weight loss and plant growth using a ch1-1 mutant that was defective of LHCs and compared this with wild-type (WT) Arabidopsis thaliana plants. Glutathione monoethyl ester (GSHmee) increased but 1-chloro-2,4 dinitrobenzene (CDNB) decreased the GSH content in the guard cells. The guard cells of the ch1-1 mutants accumulated significantly less GSH than the WT plants. The guard cells of the ch1-1 mutants also showed higher sensitivity to ABA than the WT plants. The CDNB treatment increased but the GSHmee treatment decreased the ABA sensitivity of the guard cells without affecting ABA-induced ROS production. Dark and light treatments altered the GSH content and stomatal aperture of the guard cells of ch1-1 and WT plants, irrespective of CDNB and GSHmee. The ch1-1 mutant contained fewer guard cells and displayed poor growth, late flowering and stumpy weight loss compared with the WT plants. This study suggests that defective LHCs reduced the GSH content in the guard cells and increased sensitivity to ABA, resulting in stomatal closure. Elsevier GmbH 2016-05 Article PeerReviewed image en http://eprints.unisza.edu.my/7198/1/FH02-FBIM-16-05536.jpg image en http://eprints.unisza.edu.my/7198/2/FH02-FBIM-16-06935.jpg Md. Sarwar, Jahan and Nashriyah, Mat and Mohd Nozulaidi, Nordin (2016) Light-harvesting complexes in photosystem II regulate glutathione-induced sensitivity of Arabidopsis guard cells to abscisic acid. Journal of Plant Physiology, 195. pp. 1-8. ISSN 01761617
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
English
topic GE Environmental Sciences
spellingShingle GE Environmental Sciences
Md. Sarwar, Jahan
Nashriyah, Mat
Mohd Nozulaidi, Nordin
Light-harvesting complexes in photosystem II regulate glutathione-induced sensitivity of Arabidopsis guard cells to abscisic acid
description Light-harvesting complexes (LHCs) in photosystem II (PSII) regulate glutathione (GSH) functions in plants. To investigate whether LHCs control GSH biosynthesis that modifies guard cell abscisic acid (ABA) sensitivity, we evaluated GSH content, stomatal aperture, reactive oxygen species (ROS), weight loss and plant growth using a ch1-1 mutant that was defective of LHCs and compared this with wild-type (WT) Arabidopsis thaliana plants. Glutathione monoethyl ester (GSHmee) increased but 1-chloro-2,4 dinitrobenzene (CDNB) decreased the GSH content in the guard cells. The guard cells of the ch1-1 mutants accumulated significantly less GSH than the WT plants. The guard cells of the ch1-1 mutants also showed higher sensitivity to ABA than the WT plants. The CDNB treatment increased but the GSHmee treatment decreased the ABA sensitivity of the guard cells without affecting ABA-induced ROS production. Dark and light treatments altered the GSH content and stomatal aperture of the guard cells of ch1-1 and WT plants, irrespective of CDNB and GSHmee. The ch1-1 mutant contained fewer guard cells and displayed poor growth, late flowering and stumpy weight loss compared with the WT plants. This study suggests that defective LHCs reduced the GSH content in the guard cells and increased sensitivity to ABA, resulting in stomatal closure.
format Article
author Md. Sarwar, Jahan
Nashriyah, Mat
Mohd Nozulaidi, Nordin
author_facet Md. Sarwar, Jahan
Nashriyah, Mat
Mohd Nozulaidi, Nordin
author_sort Md. Sarwar, Jahan
title Light-harvesting complexes in photosystem II regulate glutathione-induced sensitivity of Arabidopsis guard cells to abscisic acid
title_short Light-harvesting complexes in photosystem II regulate glutathione-induced sensitivity of Arabidopsis guard cells to abscisic acid
title_full Light-harvesting complexes in photosystem II regulate glutathione-induced sensitivity of Arabidopsis guard cells to abscisic acid
title_fullStr Light-harvesting complexes in photosystem II regulate glutathione-induced sensitivity of Arabidopsis guard cells to abscisic acid
title_full_unstemmed Light-harvesting complexes in photosystem II regulate glutathione-induced sensitivity of Arabidopsis guard cells to abscisic acid
title_sort light-harvesting complexes in photosystem ii regulate glutathione-induced sensitivity of arabidopsis guard cells to abscisic acid
publisher Elsevier GmbH
publishDate 2016
url http://eprints.unisza.edu.my/7198/1/FH02-FBIM-16-05536.jpg
http://eprints.unisza.edu.my/7198/2/FH02-FBIM-16-06935.jpg
http://eprints.unisza.edu.my/7198/
_version_ 1744358590211162112
score 13.211869

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