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Paracetamol Induced Liver Morphological Changes after Acute Dosing

Paracetamol, N-acetyl-p-aminophenol (APAP) belongs to a class of drugs called analgesics and antipyretics. It is considered a safe drug and available Over-The-Counter in most countries. This study was conducted to observe the effect of paracetamol on the liver of male Sprague Dawley rats. 30 rats we...

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Main Authors: Hudaa Zulfaa, Mohd Zainuddin, Laila Ruwaida, Mohd Zainuddin
Format: Article
Language:English
English
Published: 2015
Subjects:
QH301 Biology
R Medicine (General)
Online Access:http://eprints.unisza.edu.my/6860/1/FH02-FSK-15-04244.pdf
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spelling my-unisza-ir.68602022-09-13T05:21:38Z http://eprints.unisza.edu.my/6860/ Paracetamol Induced Liver Morphological Changes after Acute Dosing Hudaa Zulfaa, Mohd Zainuddin Laila Ruwaida, Mohd Zainuddin QH301 Biology R Medicine (General) Paracetamol, N-acetyl-p-aminophenol (APAP) belongs to a class of drugs called analgesics and antipyretics. It is considered a safe drug and available Over-The-Counter in most countries. This study was conducted to observe the effect of paracetamol on the liver of male Sprague Dawley rats. 30 rats were randomly divided into groups of 100mg/kg and 200mg/kg paracetamol and the control group was given saline. Treatment was given at a single dose of 1.0 ml/rat by intraperitoneal route. 3 rats were treated as normal as no treatment was given. 3 rats from each group were sacrificed after 24, 48 and 72 hours post-treatment. The liver section was stained with H and E staining and viewed under light microscope for lesion scoring. The statistical test shows that there were no significant decrease (p<0.05) in the mean weight of rats treated with paracetamol compared to saline-treated rats. The mean ratio of liver/100g body weight from rats treated with 100mg/kg and 200mg/kg paracetamol showed significant increase compared to saline-treated rats. Histological observation showed mild to moderate portal inflammation with infiltration and severe hyperemia in the 100mg/kg of paracetamol group. Liver section from rats administered with 200mg/kg paracetamol showed severe portal infiltration with hepatocytes undergoing necrosis and severe hyperemia. 200mg/kg paracetamol induced greater degree of necrosis compared to saline-treated group. The finding of this study suggested that even though paracetamol is considered a safe drug with low reported adverse effect, at higher dose (100-200mg/kg), there were significance morphological changes to the liver. 2015-04 Article PeerReviewed text en http://eprints.unisza.edu.my/6860/1/FH02-FSK-15-04244.pdf image en http://eprints.unisza.edu.my/6860/2/FH02-FSK-16-06312.jpg Hudaa Zulfaa, Mohd Zainuddin and Laila Ruwaida, Mohd Zainuddin (2015) Paracetamol Induced Liver Morphological Changes after Acute Dosing. Research Journal of Pharmacy and Technology, 8 (4). pp. 382-388. ISSN 0974-3618
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
English
topic QH301 Biology
R Medicine (General)
spellingShingle QH301 Biology
R Medicine (General)
Hudaa Zulfaa, Mohd Zainuddin
Laila Ruwaida, Mohd Zainuddin
Paracetamol Induced Liver Morphological Changes after Acute Dosing
description Paracetamol, N-acetyl-p-aminophenol (APAP) belongs to a class of drugs called analgesics and antipyretics. It is considered a safe drug and available Over-The-Counter in most countries. This study was conducted to observe the effect of paracetamol on the liver of male Sprague Dawley rats. 30 rats were randomly divided into groups of 100mg/kg and 200mg/kg paracetamol and the control group was given saline. Treatment was given at a single dose of 1.0 ml/rat by intraperitoneal route. 3 rats were treated as normal as no treatment was given. 3 rats from each group were sacrificed after 24, 48 and 72 hours post-treatment. The liver section was stained with H and E staining and viewed under light microscope for lesion scoring. The statistical test shows that there were no significant decrease (p<0.05) in the mean weight of rats treated with paracetamol compared to saline-treated rats. The mean ratio of liver/100g body weight from rats treated with 100mg/kg and 200mg/kg paracetamol showed significant increase compared to saline-treated rats. Histological observation showed mild to moderate portal inflammation with infiltration and severe hyperemia in the 100mg/kg of paracetamol group. Liver section from rats administered with 200mg/kg paracetamol showed severe portal infiltration with hepatocytes undergoing necrosis and severe hyperemia. 200mg/kg paracetamol induced greater degree of necrosis compared to saline-treated group. The finding of this study suggested that even though paracetamol is considered a safe drug with low reported adverse effect, at higher dose (100-200mg/kg), there were significance morphological changes to the liver.
format Article
author Hudaa Zulfaa, Mohd Zainuddin
Laila Ruwaida, Mohd Zainuddin
author_facet Hudaa Zulfaa, Mohd Zainuddin
Laila Ruwaida, Mohd Zainuddin
author_sort Hudaa Zulfaa, Mohd Zainuddin
title Paracetamol Induced Liver Morphological Changes after Acute Dosing
title_short Paracetamol Induced Liver Morphological Changes after Acute Dosing
title_full Paracetamol Induced Liver Morphological Changes after Acute Dosing
title_fullStr Paracetamol Induced Liver Morphological Changes after Acute Dosing
title_full_unstemmed Paracetamol Induced Liver Morphological Changes after Acute Dosing
title_sort paracetamol induced liver morphological changes after acute dosing
publishDate 2015
url http://eprints.unisza.edu.my/6860/1/FH02-FSK-15-04244.pdf
http://eprints.unisza.edu.my/6860/2/FH02-FSK-16-06312.jpg
http://eprints.unisza.edu.my/6860/
_version_ 1744358570669899776
score 13.222552

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