Staff View: Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana

Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana

Background Bacterial toxin-antitoxin systems usually comprise of a pair of genes encoding a stable toxin and its cognate labile antitoxin and are located in the chromosome or in plasmids of several bacterial species. Chromosomally-encoded toxin-antitoxin systems are involved in bacterial stress res...

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Main Authors:	Chew Chieng, Yeo, Fauziah, Abu Bakar, Jennifer Ann, Harikrishna
Format:	Article
Language:	English English English English
Published:	BioMed Central Ltd. 2015
Subjects:	QH301 Biology
Online Access:	http://eprints.unisza.edu.my/6100/1/FH02-FP-15-03325.pdf http://eprints.unisza.edu.my/6100/2/FH02-FP-15-03441.jpg http://eprints.unisza.edu.my/6100/3/FH02-FP-15-04178.jpg http://eprints.unisza.edu.my/6100/4/FH02-FP-15-04188.jpg http://eprints.unisza.edu.my/6100/
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spelling	my-unisza-ir.61002022-09-13T04:50:54Z http://eprints.unisza.edu.my/6100/ Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana Chew Chieng, Yeo Fauziah, Abu Bakar Jennifer Ann, Harikrishna QH301 Biology Background Bacterial toxin-antitoxin systems usually comprise of a pair of genes encoding a stable toxin and its cognate labile antitoxin and are located in the chromosome or in plasmids of several bacterial species. Chromosomally-encoded toxin-antitoxin systems are involved in bacterial stress responses and activation of the toxins usually leads to cell death or dormancy. Overexpression of the chromosomally-encoded YoeB toxin from the yefM-yoeB toxin-antitoxin locus of the Gram-positive bacterium Streptococcus pneumoniae has been shown to cause cell death in S. pneumoniae as well as E. coli. Results Induction of a YoeB-GFP fusion protein using a 17-β-estradiol-inducible plant expression system in Arabidopsis thaliana Col 0, was lethal in all T2 progeny. Examination of plants by fluorescent confocal microscopy showed GFP fluorescence in all parts of the leaves at 24 hours after 17-β-estradiol induction, continuing up to plant death. Quantitative RT-PCR analysis revealed that the expression of the yoeB toxin gene peaked at 3 days after induction with 17-β-estradiol, coinciding with the onset of visible effects on the plants. Moreover, we detected DNA laddering in the transgenic plants at 24 hours after yoeB induction, indicative of apoptosis. Conclusions Expression of the YoeB toxin from Streptococcus pneumoniae is lethal in Arabidopsis. We believe this is the first report of a toxin from a bacterial toxin-antitoxin system functioning in plants. The results presented here mark an important milestone towards the development of a cell ablation based bio-containment strategy, which may be useful for functional studies and for the control of spread of transgenic plants. BioMed Central Ltd. 2015-04 Article PeerReviewed text en http://eprints.unisza.edu.my/6100/1/FH02-FP-15-03325.pdf image en http://eprints.unisza.edu.my/6100/2/FH02-FP-15-03441.jpg image en http://eprints.unisza.edu.my/6100/3/FH02-FP-15-04178.jpg image en http://eprints.unisza.edu.my/6100/4/FH02-FP-15-04188.jpg Chew Chieng, Yeo and Fauziah, Abu Bakar and Jennifer Ann, Harikrishna (2015) Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana. BMC Biotechnology, 15 (1). ISSN 1472-6750
institution	Universiti Sultan Zainal Abidin
building	UNISZA Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sultan Zainal Abidin
content_source	UNISZA Institutional Repository
url_provider	https://eprints.unisza.edu.my/
language	English English English English
topic	QH301 Biology
spellingShingle	QH301 Biology Chew Chieng, Yeo Fauziah, Abu Bakar Jennifer Ann, Harikrishna Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana
description	Background Bacterial toxin-antitoxin systems usually comprise of a pair of genes encoding a stable toxin and its cognate labile antitoxin and are located in the chromosome or in plasmids of several bacterial species. Chromosomally-encoded toxin-antitoxin systems are involved in bacterial stress responses and activation of the toxins usually leads to cell death or dormancy. Overexpression of the chromosomally-encoded YoeB toxin from the yefM-yoeB toxin-antitoxin locus of the Gram-positive bacterium Streptococcus pneumoniae has been shown to cause cell death in S. pneumoniae as well as E. coli. Results Induction of a YoeB-GFP fusion protein using a 17-β-estradiol-inducible plant expression system in Arabidopsis thaliana Col 0, was lethal in all T2 progeny. Examination of plants by fluorescent confocal microscopy showed GFP fluorescence in all parts of the leaves at 24 hours after 17-β-estradiol induction, continuing up to plant death. Quantitative RT-PCR analysis revealed that the expression of the yoeB toxin gene peaked at 3 days after induction with 17-β-estradiol, coinciding with the onset of visible effects on the plants. Moreover, we detected DNA laddering in the transgenic plants at 24 hours after yoeB induction, indicative of apoptosis. Conclusions Expression of the YoeB toxin from Streptococcus pneumoniae is lethal in Arabidopsis. We believe this is the first report of a toxin from a bacterial toxin-antitoxin system functioning in plants. The results presented here mark an important milestone towards the development of a cell ablation based bio-containment strategy, which may be useful for functional studies and for the control of spread of transgenic plants.
format	Article
author	Chew Chieng, Yeo Fauziah, Abu Bakar Jennifer Ann, Harikrishna
author_facet	Chew Chieng, Yeo Fauziah, Abu Bakar Jennifer Ann, Harikrishna
author_sort	Chew Chieng, Yeo
title	Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana
title_short	Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana
title_full	Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana
title_fullStr	Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana
title_full_unstemmed	Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana
title_sort	expression of the streptococcus pneumoniae yoeb chromosomal toxin gene causes cell death in the model plant arabidopsis thaliana
publisher	BioMed Central Ltd.
publishDate	2015
url	http://eprints.unisza.edu.my/6100/1/FH02-FP-15-03325.pdf http://eprints.unisza.edu.my/6100/2/FH02-FP-15-03441.jpg http://eprints.unisza.edu.my/6100/3/FH02-FP-15-04178.jpg http://eprints.unisza.edu.my/6100/4/FH02-FP-15-04188.jpg http://eprints.unisza.edu.my/6100/
_version_	1744358548084621312
score	13.211869

Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana

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