Somatic Embryos Multiplication of Eurycoma longifolia Jack in vitro by using Temporary Immersion System RITA
Temporary immersion system, Recipient for Automated Temporary Immersion® (RITA® ), is one of the innovative systems that allow the production of large number of somatic embryos or plantlets in in vitro plant propagation. It has been used widely to avoid associated problems with in vitro propagat...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/597/1/FH03-FBIM-16-04857.pdf http://eprints.unisza.edu.my/597/ |
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| Summary: | Temporary immersion system, Recipient for Automated Temporary Immersion®
(RITA®
), is one of
the innovative systems that allow the production of large number of somatic embryos or plantlets in in vitro
plant propagation. It has been used widely to avoid associated problems with in vitro propagation such as
low multiplication rate of somatic embryos and hyperhydricity. Thus, an attempt was made to investigate the
optimal parameters; immersion time and immersion frequency, for the multiplication of direct somatic
embryogenesis from cotyledon culture of E. longifolia Jack by using RITA®
. The basal media used for the
multiplication of somatic embryogenesis was Modified Murashige and Skoog (MS) media supplemented with
0.2 mg/L indole-3-butyric acid (IBA) + 0.1 mg/L Zeatin + 0.12 mg/L thidiazuron (TDZ) + 0.1 g/L activated
charcoal. Four periods of immersion time (1, 5, 10 and 15 minutes every 4 hours) were evaluated for the
efficiency in somatic embryos multiplication. In order to optimize repetitive somatic embryogenesis, three
different immersion frequencies (5 min immersion every 2, 4 and 8 hours) were applied. The semi solid
media was also studied to be compared as control. One Way Analysis of Variance (ANOVA) showed that the
highest number of secondary embryos (69.67 ± 9.73) was found significant when immersing the globular,
primary embryos for 5 minutes every 4 hours as compared to other treatments tested. Apart from that, there
is no significant difference for all the immersion frequencies investigated. The secondary somatic embryos
obtained in this study could be further used for development of plantlet regeneration system of E. longifolia. |
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