Cover Image

Comparative Study of Three Different Extenders on Fresh, Chilled and Cryopreserved Mafriwal Bull’s Sperm

The aim of this study is to study the effects of different types of extender and to determine the effects of cryopreservation process (before chilling, after chilling, after cryopreservation) on sperm characteristics and sperm morphology of Mafriwal bulls. Three natural based extender (Aloe vera bas...

Full description

Saved in:
Bibliographic Details
Main Authors: Asmad, Kari, Nur Amalina, N, Zawawi, I
Format: Article
Language:English
Published: 2018
Subjects:
SF Animal culture
Online Access:http://eprints.unisza.edu.my/5749/1/FH02-FBIM-19-24111.pdf
http://eprints.unisza.edu.my/5749/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-unisza-ir.5749
record_format eprints
spelling my-unisza-ir.57492022-02-23T08:01:35Z http://eprints.unisza.edu.my/5749/ Comparative Study of Three Different Extenders on Fresh, Chilled and Cryopreserved Mafriwal Bull’s Sperm Asmad, Kari Nur Amalina, N Zawawi, I SF Animal culture The aim of this study is to study the effects of different types of extender and to determine the effects of cryopreservation process (before chilling, after chilling, after cryopreservation) on sperm characteristics and sperm morphology of Mafriwal bulls. Three natural based extender (Aloe vera based extender (AVBX), honey based extender (HBX), egg yolk based extender (EYBX)) and a control (Bioxcell®) were used as extender and compared for different semen quality parameters that can be assessed under routine laboratory condition. Se-men samples were collected from three sexually matured Mafriwal bulls by using artificial vagina (AV). Samples collected were subject-ed to four different treatments and was evaluated after dilution with extenders. After diluted, the samples were chilled for 2 hours in 4°C. The semen samples were then packed into 0.25 ml straw for further cryopreservation process. After 24 hours, the cryopreserved semen samples were evaluated. Eosin nigrosin smearing were done to evaluate sperm morphology. The EYBX resulted in the highest percent-age of almost all parameters tested in the study followed by Bioxcell®, AVBX and HBX. Semen treated with EYBX and Bioxcell® shows no significant different (P>0.05). All treatment shows decrease of sperm characteristics after chilling and frozen-thawed but AVBX and HBX shows rapid depletion of sperm characteristics (P<0.05) after chilling process. This study suggests that EYBX is the most suitable extender for cryopreservation Mafriwal bull’s semen. However, further study is warranted to investigate the optimum con-centration of other raw materials (i.e. Aloe vera and honey) in extender for semen cryopreservation. 2018-12 Article PeerReviewed text en http://eprints.unisza.edu.my/5749/1/FH02-FBIM-19-24111.pdf Asmad, Kari and Nur Amalina, N and Zawawi, I (2018) Comparative Study of Three Different Extenders on Fresh, Chilled and Cryopreserved Mafriwal Bull’s Sperm. International Journal of Engineering & Technology, 7 (4). pp. 127-134. ISSN 2227-524X
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic SF Animal culture
spellingShingle SF Animal culture
Asmad, Kari
Nur Amalina, N
Zawawi, I
Comparative Study of Three Different Extenders on Fresh, Chilled and Cryopreserved Mafriwal Bull’s Sperm
description The aim of this study is to study the effects of different types of extender and to determine the effects of cryopreservation process (before chilling, after chilling, after cryopreservation) on sperm characteristics and sperm morphology of Mafriwal bulls. Three natural based extender (Aloe vera based extender (AVBX), honey based extender (HBX), egg yolk based extender (EYBX)) and a control (Bioxcell®) were used as extender and compared for different semen quality parameters that can be assessed under routine laboratory condition. Se-men samples were collected from three sexually matured Mafriwal bulls by using artificial vagina (AV). Samples collected were subject-ed to four different treatments and was evaluated after dilution with extenders. After diluted, the samples were chilled for 2 hours in 4°C. The semen samples were then packed into 0.25 ml straw for further cryopreservation process. After 24 hours, the cryopreserved semen samples were evaluated. Eosin nigrosin smearing were done to evaluate sperm morphology. The EYBX resulted in the highest percent-age of almost all parameters tested in the study followed by Bioxcell®, AVBX and HBX. Semen treated with EYBX and Bioxcell® shows no significant different (P>0.05). All treatment shows decrease of sperm characteristics after chilling and frozen-thawed but AVBX and HBX shows rapid depletion of sperm characteristics (P<0.05) after chilling process. This study suggests that EYBX is the most suitable extender for cryopreservation Mafriwal bull’s semen. However, further study is warranted to investigate the optimum con-centration of other raw materials (i.e. Aloe vera and honey) in extender for semen cryopreservation.
format Article
author Asmad, Kari
Nur Amalina, N
Zawawi, I
author_facet Asmad, Kari
Nur Amalina, N
Zawawi, I
author_sort Asmad, Kari
title Comparative Study of Three Different Extenders on Fresh, Chilled and Cryopreserved Mafriwal Bull’s Sperm
title_short Comparative Study of Three Different Extenders on Fresh, Chilled and Cryopreserved Mafriwal Bull’s Sperm
title_full Comparative Study of Three Different Extenders on Fresh, Chilled and Cryopreserved Mafriwal Bull’s Sperm
title_fullStr Comparative Study of Three Different Extenders on Fresh, Chilled and Cryopreserved Mafriwal Bull’s Sperm
title_full_unstemmed Comparative Study of Three Different Extenders on Fresh, Chilled and Cryopreserved Mafriwal Bull’s Sperm
title_sort comparative study of three different extenders on fresh, chilled and cryopreserved mafriwal bull’s sperm
publishDate 2018
url http://eprints.unisza.edu.my/5749/1/FH02-FBIM-19-24111.pdf
http://eprints.unisza.edu.my/5749/
_version_ 1725976990315446272
score 13.211869

Similar Items