Cover Image

Callus Induction from Leaf Explants of Mas Cotek (Ficus Deltoidea var. bilobata) Treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)

The objective of this study was to observe the effect of 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-trichloropicolinic acid (Picloram) on callus induction of Mas Cotek (Ficus deltoidea var bilobata) explant. Murashige and Skoog (MS) nutrient media supplemented with 6-Benzylaminopurine (BAP) and 4...

Full description

Saved in:
Bibliographic Details
Main Authors: Norshazila, Shahidan, Nashriyah, Mat, Azrina, Ismail, Rashidi, Othman
Format: Article
Language:English
Published: 2018
Subjects:
Q Science (General)
SB Plant culture
Online Access:http://eprints.unisza.edu.my/5737/1/FH02-FBIM-19-24118.pdf
http://eprints.unisza.edu.my/5737/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-unisza-ir.5737
record_format eprints
spelling my-unisza-ir.57372022-02-23T07:28:48Z http://eprints.unisza.edu.my/5737/ Callus Induction from Leaf Explants of Mas Cotek (Ficus Deltoidea var. bilobata) Treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram) Norshazila, Shahidan Nashriyah, Mat Azrina, Ismail Rashidi, Othman Q Science (General) SB Plant culture The objective of this study was to observe the effect of 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-trichloropicolinic acid (Picloram) on callus induction of Mas Cotek (Ficus deltoidea var bilobata) explant. Murashige and Skoog (MS) nutrient media supplemented with 6-Benzylaminopurine (BAP) and 4-amino-3,5,6-trichloro picolinic acid (Picloram) each at different concentrations of 0 mg/l, 0.5 mg/l , 1.5 mg/l and 3.0 mg/l, respectively. The control and other six treatments gave negative responses to the MS media supplemented with various concentrations of BAP and Picloram. Most of the callus began to form at day 16 up to day 30 with treatment. Hard and yellowish callus with moderate growth rate was obtained from explants cultured on MS medium supplemented with 0.5 mg/ l BAP and 1.5 mg/l Picloram, wherein the callus formation was recorded at 47 %. Hard and greenish callus was obtained from medium treated with 0.5 mg/l BAP and 0.5 mg/l Picloram after 30 days of treatment with callus formation of 26.67 %. Medium supplemented with 0.5 mg/l of BAP hormone and 1.5 mg/l of Picloram hormone displayed the best growth rate of callus and was the most suitable medium for callus induction 2018-12 Article PeerReviewed text en http://eprints.unisza.edu.my/5737/1/FH02-FBIM-19-24118.pdf Norshazila, Shahidan and Nashriyah, Mat and Azrina, Ismail and Rashidi, Othman (2018) Callus Induction from Leaf Explants of Mas Cotek (Ficus Deltoidea var. bilobata) Treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram). International Journal of Engineering & Technology, 7 (4). pp. 164-167. ISSN 2227-524X
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic Q Science (General)
SB Plant culture
spellingShingle Q Science (General)
SB Plant culture
Norshazila, Shahidan
Nashriyah, Mat
Azrina, Ismail
Rashidi, Othman
Callus Induction from Leaf Explants of Mas Cotek (Ficus Deltoidea var. bilobata) Treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
description The objective of this study was to observe the effect of 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-trichloropicolinic acid (Picloram) on callus induction of Mas Cotek (Ficus deltoidea var bilobata) explant. Murashige and Skoog (MS) nutrient media supplemented with 6-Benzylaminopurine (BAP) and 4-amino-3,5,6-trichloro picolinic acid (Picloram) each at different concentrations of 0 mg/l, 0.5 mg/l , 1.5 mg/l and 3.0 mg/l, respectively. The control and other six treatments gave negative responses to the MS media supplemented with various concentrations of BAP and Picloram. Most of the callus began to form at day 16 up to day 30 with treatment. Hard and yellowish callus with moderate growth rate was obtained from explants cultured on MS medium supplemented with 0.5 mg/ l BAP and 1.5 mg/l Picloram, wherein the callus formation was recorded at 47 %. Hard and greenish callus was obtained from medium treated with 0.5 mg/l BAP and 0.5 mg/l Picloram after 30 days of treatment with callus formation of 26.67 %. Medium supplemented with 0.5 mg/l of BAP hormone and 1.5 mg/l of Picloram hormone displayed the best growth rate of callus and was the most suitable medium for callus induction
format Article
author Norshazila, Shahidan
Nashriyah, Mat
Azrina, Ismail
Rashidi, Othman
author_facet Norshazila, Shahidan
Nashriyah, Mat
Azrina, Ismail
Rashidi, Othman
author_sort Norshazila, Shahidan
title Callus Induction from Leaf Explants of Mas Cotek (Ficus Deltoidea var. bilobata) Treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
title_short Callus Induction from Leaf Explants of Mas Cotek (Ficus Deltoidea var. bilobata) Treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
title_full Callus Induction from Leaf Explants of Mas Cotek (Ficus Deltoidea var. bilobata) Treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
title_fullStr Callus Induction from Leaf Explants of Mas Cotek (Ficus Deltoidea var. bilobata) Treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
title_full_unstemmed Callus Induction from Leaf Explants of Mas Cotek (Ficus Deltoidea var. bilobata) Treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
title_sort callus induction from leaf explants of mas cotek (ficus deltoidea var. bilobata) treated with 6-benzylaminopurine (bap) and 4-amino-3, 5, 6-trichloropicolinic acid (picloram)
publishDate 2018
url http://eprints.unisza.edu.my/5737/1/FH02-FBIM-19-24118.pdf
http://eprints.unisza.edu.my/5737/
_version_ 1725976988771942400
score 13.211869

Similar Items